Impaired G2/M cell cycle arrest induces apoptosis in pyruvate carboxylase knockdown MDA-MB-231 cells
Biochemistry and Biophysics Reports, 2021 Background: Previous studies showed that suppression of pyruvate carboxylase (PC) expression in highly invasive breast cancer cell line, MDA-MB-231 inhibits cell growth as a consequence of the impaired cellular biosynthesis. However, the precise cellular
Khanti Rattanapornsompong +6 more
doaj +1 more source
Serine biosynthesis with one carbon catabolism represents a novel pathway for ATP generation in cells using alternative glycolysis with zero net ATP production [PDF]
, 2011 Recent experimental evidence indicates that some cancer cells have an alternative glycolysis pathway with net zero ATP production, implying that upregulation of glycolysis in these cells may not be related to the generation of ATP.
Alexei Vazquez +2 more
core +5 more sources
The citrate cleavage pathway and lipogenesis in rat adipose tissue: replenishment of oxaloacetate
Journal of Lipid Research, 1967 Fatty acid synthesis via the citrate cleavage pathway requires the continual replenishment of oxaloacetate within the mitochondria, probably by carboxylation of pyruvate.Malic enzyme, although present in adipose tissue, is completely localized in the ...
F.J. Ballard, Richard W. Hanson
doaj +1 more source
Insights Into the Carboxyltransferase Reaction of Pyruvate Carboxylase From the Structures of Bound Product and Intermediate Analogs [PDF]
, 2013 Pyruvate carboxylase (PC) is a biotin-dependent enzyme that catalyzes the MgATP- and bicarbonate-dependent carboxylation of pyruvate to oxaloacetate, an important anaplerotic reaction in central metabolism.
Lietzan, Adam D., Maurice, Martin St.
core +2 more sources
Assessment of hepatic pyruvate carboxylase activity using hyperpolarized [1‐13C]‐l‐lactate
Magnetic Resonance in Medicine, 2020 To evaluate the utility of hyperpolarized [1‐13C]‐l‐lactate to detect hepatic pyruvate carboxylase activity in vivo under fed and fasted conditions.
Jun Chen +4 more
semanticscholar +1 more source
Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells
Nature Communications, 2023 Deregulated oxidative metabolism is a hallmark of leukaemia. While tyrosine kinase inhibitors (TKIs) such as imatinib have increased survival of chronic myeloid leukaemia (CML) patients, they fail to eradicate disease-initiating leukemic stem cells (LSCs)
Kevin M. Rattigan +18 more
doaj +1 more source
Minimizing acetate formation in E. coli fermentations [PDF]
, 2007 Escherichia coli remains the best established production organisms in industrial biotechnology. However, during aerobic fermentation runs at high growth rates, considerable amounts of acetate are accumulated as by-product.
De Maeseneire, Sofie +3 more
core +2 more sources
Pre-hybridisation: an efficient way of suppressing endogenous biotin-binding activity inherent to biotin–streptavidin detection system [PDF]
, 2014 Endogenous biotin or biotinylated protein binding activity is a major drawback to biotin-avidin/streptavidin detection system. The avidin/streptavidin conjugate used to detect the complex of the biotinylated secondary antibody and the primary antibody ...
Andrew Thompsett +22 more
core +1 more source
AIMS Bioengineering, 2016 Glycerol is a major by-product of the biodiesel industry and its transformation into value-added products is an ongoing technological challenge. Here we report on the ability of the nutritionally-versatile Pseudomonas fluorescens to synthesize copious ...
Azhar A. Alhasawi, Vasu D. Appanna
doaj +1 more source
Deletion of Pyruvate Carboxylase in Tubular Epithelial Cell Promotes Renal Fibrosis by Regulating SQOR/cGAS/STING-Mediated Glycolysis. [PDF]
Adv Sci (Weinh)Huang H +14 more
europepmc +3 more sources