Results 21 to 30 of about 1,004,764 (341)

DIRECT RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWABS WITHOUT AN RNA EXTRACTION STEP

bioRxiv, 2020
The ongoing SARS-CoV-2 pandemic has caused an unprecedented need for rapid diagnostic screening [1]. The primary assay employed is a reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay that requires the use of an RNA extraction ...
E. Bruce, S. Tighe, Jessica J Hoffman, Pheobe Laaguiby, D. Gerrard, S. Diehl, D. Leonard, C. Huston, B. Kirkpatrick, Jessica W. Crothers, J. Dragon, J. Botten   +11 more
semanticscholar   +2 more sources

Comparison of species-specific qPCR and metabarcoding methods to detect small pelagic fish distribution from open ocean environmental DNA

PLoS ONE, 2022
Environmental DNA (eDNA) is increasingly used to noninvasively monitor aquatic animals in freshwater and coastal areas. However, the use of eDNA in the open ocean (hereafter referred to OceanDNA) is still limited because of the sparse distribution of ...
Zeshu Yu, S. Ito, M. Wong, Susumu Yoshizawa, Jun Inoue, S. Itoh, Ryuji Yukami, K. Ishikawa, Chenying Guo, M. Ijichi, S. Hyodo   +10 more
semanticscholar   +1 more source

Clinical Evaluation of Self-Collected Saliva by Quantitative Reverse Transcription-PCR (RT-qPCR), Direct RT-qPCR, Reverse Transcription–Loop-Mediated Isothermal Amplification, and a Rapid Antigen Test To Diagnose COVID-19

Journal of Clinical Microbiology, 2020
The clinical performances of six molecular diagnostic tests and a rapid antigen test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were clinically evaluated for the diagnosis of coronavirus disease 2019 (COVID-19) in self-collected ...
M. Nagura-Ikeda, K. Imai, S. Tabata, K. Miyoshi, Nami Murahara, Tsukasa Mizuno, M. Horiuchi, Kento Kato, Yoshitaka Imoto, Makiko Iwata, S. Mimura, Toshimitsu Ito, Kaku Tamura, Y. Kato   +13 more
semanticscholar   +1 more source

Improved Subtyping of Avian Influenza Viruses Using an RT-qPCR-Based Low Density Array: ‘Riems Influenza a Typing Array’, Version 2 (RITA-2)

Viruses, 2022
Avian influenza virus (AIV) variants emerge frequently, which challenges rapid diagnosis. Appropriate diagnosis reaching the sub- and pathotype level is the basis of combatting notifiable AIV infections.
Kareem E Hassan, Ann Kathrin Ahrens, Ahmed Ali, M. El-kady, H. Hafez, T. Mettenleiter, M. Beer, T. Harder   +7 more
semanticscholar   +1 more source

High-throughput qPCR and 16S rRNA gene amplicon sequencing as complementary methods for the investigation of the cheese microbiota

BMC Microbiology, 2022
Background Next-generation sequencing (NGS) methods and especially 16S rRNA gene amplicon sequencing have become indispensable tools in microbial ecology.
M. Dreier, M. Meola, H. Berthoud, N. Shani, D. Wechsler, P. Junier   +5 more
semanticscholar   +1 more source

Efficiency-correction is required for accurate qPCR analysis and reporting.

Clinical Chemistry, 2021
BACKGROUND Quantitative PCR (qPCR) aims to measure the DNA or RNA concentration in diagnostic and biological samples based on the Cq value observed in the amplification curves. Results of qPCR experiments are regularly calculated as if all assays are 100%
J. Ruijter, R. Barnewall, I. Marsh, Andrew N Szentirmay, J. Quinn, R. van Houdt, Q. Gunst, M. V. D. van den Hoff   +7 more
semanticscholar   +1 more source

PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions

Analytical and Bioanalytical Chemistry, 2020
DNA analysis has seen an incredible development in terms of instrumentation, assays and applications over the last years. Massively parallel sequencing (MPS) and digital PCR are now broadly applied in research and diagnostics, and quantitative PCR is ...
Maja Sidstedt, P. Rådström, J. Hedman   +2 more
semanticscholar   +1 more source

Use and Misuse of Cq in qPCR Data Analysis and Reporting

Life, 2021
In the analysis of quantitative PCR (qPCR) data, the quantification cycle (Cq) indicates the position of the amplification curve with respect to the cycle axis.
A. Ruiz-Villalba, J. Ruijter, M. V. D. van den Hoff   +2 more
semanticscholar   +1 more source

Quantitative SARS-CoV-2 Alpha Variant B.1.1.7 Tracking in Wastewater by Allele-Specific RT-qPCR

Environmental Science and Technology Letters, 2021
The critical need for surveillance of SARS-CoV-2 variants of concern has prompted the development of methods that can track variants in wastewater. Here, we develop and present an open-source method based on allele-specific RT-qPCR (AS RT-qPCR) that ...
W. Lee, Maxim Imakaev, F. Armas, K. A. McElroy, X. Gu, C. Duvallet, F. Chandra, Hongjie Chen, M. Leifels, S. Mendola, R. Floyd-O’Sullivan, M. Powell, Shane T. Wilson, Karl L. J. Berge, C. Y. Lim, Fuqing Wu, A. Xiao, K. Moniz, N. Ghaeli, M. Matus, Janelle Thompson, E. Alm   +21 more
semanticscholar   +1 more source

RT-qPCR Testing of SARS-CoV-2: A Primer

International Journal of Molecular Sciences, 2020
Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely ...
S. Bustin, T. Nolan
semanticscholar   +1 more source