Results 21 to 30 of about 596,924 (249)

Microbial water quality of pond Alleben (Gaziantep, Turkey) in winter and climatic changes in the region

open access: yesJournal of Water and Climate Change, 2023
This study assessed the microbial quality of water sources in winter by microbiological methods and molecular-based quantitative polymerase chain reaction (qPCR) and potential future bacterial loads in the Alleben pond based on the increase in water ...
Hatice Aysun Mercimek Takci   +3 more
doaj   +1 more source

Biochar Enhances Nitrous Oxide Reduction in Acidic but Not in Near-Neutral pH Soil

open access: yesSoil Systems, 2019
We quantified nitrous oxide (N2O) fluxes and total denitrification (N2O + N2) in an acidic (Ferralsol) and a near-neutral pH soil (Cambisol) to determine whether biochar’s alkalinization effect could be the mechanism inducing potential reductions ...
Engil Isadora Pujol Pereira   +4 more
doaj   +1 more source

qPCR v1 [PDF]

open access: yes, 2018

Lele Sun   +3 more
openaire   +1 more source

qPCR, dPCR, NGS – A journey [PDF]

open access: yesBiomolecular Detection and Quantification, 2015
Scientific conferences fulfil many roles, but one of the most important ones is that they help shape the direction in which a scientific discipline grows by promoting person-to-person exchanges of information, ideas and constructive criticisms between scientists from different backgrounds.
Huggett, Jim   +2 more
openaire   +3 more sources

Semi-Quantitative, Duplexed qPCR Assay for the Detection of Leishmania spp. Using Bisulphite Conversion Technology

open access: yesTropical Medicine and Infectious Disease, 2019
Leishmaniasis is caused by the flagellated protozoan Leishmania, and is a neglected tropical disease (NTD), as defined by the World Health Organisation (WHO).
Ineka Gow   +4 more
doaj   +1 more source

qPCR primer design revisited

open access: yesBiomolecular Detection and Quantification, 2017
Primers are arguably the single most critical components of any PCR assay, as their properties control the exquisite specificity and sensitivity that make this method uniquely powerful. Consequently, poor design combined with failure to optimise reaction conditions is likely to result in reduced technical precision and false positive or negative ...
Bustin, Stephen A., Huggett, Jim
openaire   +2 more sources

Comparing the stemness and morphobiometry of spermatogonial stem cells from Doom pig on different days of culture

open access: yesCzech Journal of Animal Science, 2020
The present study was conducted to compare the stemness and morphobiometry of spermatogonial stem cells (SSCs) from the Doom pig on different days of culture (9th, 30th and 65th day) for the development of long-term culture method.
Arpana Das   +8 more
doaj   +1 more source

Tuz Stresinin Hafifletilmesinde L-Argininin Zea mays’da Antioksidan Enzim Aktivitesi Üzerine Etkisi

open access: yesTurkish Journal of Agriculture: Food Science and Technology
Arjinin, bitkilerde stres metabolizmasında yer alan çeşitli metabolitlerin öncülüğünü yapan bir aminoasit olmasının yanı sıra, bitkilerin çevresel streslere yanıtını modüle edebilen bir sinyal molekülüdür.
Esra Arslan Yüksel
doaj   +1 more source

Quantification of Pneumocystis jirovecii: Cross-Platform Comparison of One qPCR Assay with Leading Platforms and Six Master Mixes

open access: yesJournal of Fungi, 2019
Diagnosis of Pneumocystis jirovecii pneumonia relies on nucleic acid quantification in respiratory samples. Lack of standardization among molecular assays results in significant differences among assays/centers.
Sarah Dellière   +5 more
doaj   +1 more source

Enhancing gene expression studies in bovine embryos fertilized in vitro: Identifying stable reference genes across blastocysts with different developmental speeds

open access: yesThe Journal of Reproduction and Development
In vitro fertilization (IVF) is crucial for livestock reproduction; however, pregnancy rates after embryo transfer vary depending on the developmental speeds of the embryos. Although quantitative PCR (qPCR) is used to predict developmental potential, its
Sung-Ho KIM   +9 more
doaj   +1 more source

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