Results 161 to 170 of about 498,441 (308)

Using real-time qPCR to evaluate RNAi-mediated gene silencing [PDF]

, 2009
De Clercq, S, De Paepe, Anne, Marine, J C, Mestdagh, Pieter, Pattyn, Filip, Speleman, Franki, Van Maerken, Tom, Vandesompele, Jo, Yigit, Nurten   +8 more
core   +2 more sources

A Modular Mechanistic In Silico Model for In Vitro Transcription Process Yield and Product Quality Prediction

Biotechnology and Bioengineering, EarlyView.
ABSTRACT In vitro transcription (IVT) plays a critical role in the manufacture of mRNA vaccines and therapeutics. Optimizing mRNA yield and ensuring product quality, such as capping efficiency and integrity, are essential but mechanistically complex. This study presents a modular mechanistic model of the IVT process to advance scientific understanding ...
Keqi Wang, Keilung Choy, Eli Reiser, Jinxiang Pei, Hua Zheng, Aparajita Dasgupta, Fuqiang Cheng, Guogang Dong, Bhanu Chandra Mulukutla, Joshua Mannheimer, Carolyn Huang, Hooman Farsani, Wei Xie   +12 more
wiley   +1 more source

Targeting Apolipoprotein E4 rescues photoreceptor degeneration by inhibiting the disease-associated microglia activation in experimental retinal detachment. [PDF]

J Neuroinflammation
Li X, Fan N, Yan Y, Ren M, Wu H, Dong K.
europepmc   +1 more source

Detergent Virus Inactivation in Chromatography Systems

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Detergent treatment is a widely utilized virus‐inactivation step in therapeutic protein manufacturing to safeguard products. Traditionally, this operation is performed in an incubation vessel in batch mode. In this investigation, a methodology was developed to enable virus inactivation via a post‐load, detergent‐containing wash within a bind ...
Kang Cai, Etienne Utiger, Chris Afdahl, Rayan Zamat, Jennifer Anderson, Yeldys De Armas, Gisela Ferreira   +6 more
wiley   +1 more source

Dried blood spot analysis in antidoping: Technical challenges, analytical advances, and future perspectives

Bulletin of the Korean Chemical Society, EarlyView.
Dried blood spot (DBS) analysis enables minimally invasive blood collection with strong analyte stability and simplified logistics, supporting anti‐doping applications beyond traditional matrices. Recent advances address hematocrit, volume variability, and matrix effects while expanding coverage to steroid esters, World Anti‐Doping Agency‐listed ...
Jihyun Yoon, Jiyeong Hong, Hanbin Oh, Chi Hwan Lee, Ki Hun Kim   +4 more
wiley   +1 more source

Chloronitramide Anion Quantitation in Tap Waters by Ion Chromatography with Electrical Conductivity and Ultraviolet Absorbance Detection. [PDF]

Environ Sci Technol Lett
Thornhill JA, Laszakovits JR, Johnson BE, Chimka JR, Fairey JL.   +4 more
europepmc   +1 more source

Homo‐/Heterodimeric Substrates Bias the Type and Multiplicity of Hydroxamic Acid Chelators Assembled by a NIS Synthetase DesD

Chemistry – A European Journal, EarlyView.
Chemoenzymatic reactions using the siderophore synthetase DesD from Salinispora tropica and hydroxamic acid substrates (native, non‐native) with different multiplicities (monomer, dimer) and components (homo‐/heterodimer) followed different reaction trajectories, which biased the architectures of the chelator major products and indicated DesD contains ...
Callum A. Rosser, Todd E. Markham, Rachel Codd   +2 more
wiley   +1 more source

HNF4A P2 isoform alleviates kidney fibrosis by inhibiting dedifferentiation of proximal tubular cells through JAG1/NOTCH signaling. [PDF]

Cell Mol Biol Lett
Jiang G, Lu X, Cao R, Zhang H, Gao Y, Lu K, Zhang L, Zhang G, Wu J, Xu B, Zhong J, Sun J, Chen M, Chen S.   +13 more
europepmc   +1 more source

The Influence of Drosophila Spire and Myosin V During Mid‐Oogenesis Is Independent of Their Direct Interaction

Cytoskeleton, EarlyView.
ABSTRACT Cooperativity between cytoskeletal proteins is crucial for spatiotemporal coordination in biological processes, like oogenesis. In mammalian and Drosophila oogenesis, proper assembly and function of actin networks require coordination between actin assembly factors Spire and formins, as well as actin‐associated proteins like myosins and Rab ...
Joseph Y. Ong, Hannah M. Bailey, Emma Carley, Michelle Panzica, Merin Rixen, Neil Billington, Rong Liu, Margot E. Quinlan   +7 more
wiley   +1 more source

Impact of Column Load and Signal-to-Noise Threshold on the Accuracy and Repeatability of Jet A Hydrocarbon Profiling via GC×GC/FID. [PDF]

J Sep Sci
Modereger BA, Caceres-Martinez LE, Peretich ME, Kenttämaa HI, Kilaz G.   +4 more
europepmc   +1 more source