Results 1 to 10 of about 1,061,192 (206)

Quantitative analysis of the dystrophin gene by real-time PCR [PDF]

Archives of Biological Sciences, 2012
Duchenne and Becker muscular dystrophy (DMD/BMD) are severe X-linked neuromuscular disorders caused by mutations in the dystrophin gene. Our aim was to optimize a quantitative real-time PCR method based on SYBR® Green I chemistry for routine ...
Maksimovic Nela, Anđelkovic Ana, Milic-Rasic Vedrana, Rakocevic-Stojanovic Vidosava, Kastratovic-Kotlica Biljana, Brankovic S., Damnjanovic Tatjana, Jekic Biljana, Bunjevacki Vera, Lukovic Ljiljana, Perovic Dijana, Cvjeticanin Suzana, Novakovic Ivana   +12 more
doaj   +4 more sources

Real-Time PCR Assay for Quantitative Mismatch Detection [PDF]

BioTechniques, 2003
We describe here a quantitative realtime PCR assay for the detection of single-base-pair differences that does not require fluorescently labeled gene-specific probes or complicated primer combinations.
L. Shively, L. Chang, J.M. LeBon, Q. Liu, A.D. Riggs, J. Singer-Sam   +5 more
doaj   +3 more sources

Quantitative real time PCR for distinction between Pneumocystis jirovecii infection/colonization in hospitalized patients [PDF]

Frontiers in Cellular and Infection Microbiology
BackgroundIdentification of the opportunistic fungus Pneumocystis jirovecii in respiratory specimens presents challenges, particularly in differentiating between colonization and active infection.
Faezeh Rouhi, Mahzad Erami, Sepide Rastgufar, Maryam Jahani, Shima Aboutalebian, Shima Aboutalebian, Sajedeh Soltani, Hamed Fakhim, Hossein Mirhendi, Hossein Mirhendi   +9 more
doaj   +2 more sources

Evaluation of RNA extraction methods in rice and their application in expression analysis of resistance genes against Magnaporthe oryzae [PDF]

Biotechnology & Biotechnological Equipment, 2017
Extraction of RNA of high quality and integrity is essential for gene expression studies and all downstream RNA-based techniques. The leaves of 16 merit Malaysian rice varieties were used to isolate total RNA using five different methods.
Parisa Azizi, Mohd Y. Rafii, Maziah Mahmood, Siti Nor Akmar Abdullah, Mohamed Musa Hanafi, Muhammad Abdul Latif, Mahbod Sahebi, Sadegh Ashkani   +7 more
doaj   +3 more sources

Gene Dosage by Quantitative Real-Time PCR

BioTechniques, 1999
J.-L. Boulay, J. Reuter, R. Ritschard, L. Terracciano, R. Herrmann, C. Rochlitz   +5 more
doaj   +3 more sources

Real time quantitative PCR. [PDF]

Genome Research, 1996
We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies.
C A, Heid, J, Stevens, K J, Livak, P M, Williams   +3 more
openaire   +2 more sources

An inter-laboratory validation of a real time PCR assay to measure host excretion of bacterial pathogens, particularly of mycobacterium bovis [PDF]

, 2011
Advances in the diagnosis of Mycobacterium bovis infection in wildlife hosts may benefit the development of sustainable approaches to the management of bovine tuberculosis in cattle.
Travis, Emma Rachel, Gaze, William H., Pontiroli, Alessandra, Sweeney, Francis P., Porter, David, Mason, Sam, Keeling, Matthew James, Jones, Rebecca M., Sawyer, Jason, Aranaz, Alicia, Castellanos Rizaldos, Elena, Cork, Jennifer, Delahay, Richard J., Wilson, Gavin J., Hewinson, R. Glyn, Courtenay, Orin, Wellington, E. M. H.   +16 more
core   +6 more sources

Genomic and Transcriptional Characteristics of Strain Rum-meliibacillus sp. TYF-LIM-RU47 with an Aptitude of Directly Producing Acetoin from Lignocellulose

Fermentation, 2022
Rummeliibacillus sp. TYF-LIM-RU47, isolated from the fermentation substrate of grain vinegar, could produce acetoin using a variety of carbon sources, including pentose, hexose and lignocellulose.
Guoyang Feng, Xiaojun Fan, Yanna Liang, Chen Li, Junde Xing, Yongji He   +5 more
doaj   +1 more source

Detection of 1p19q Deletion by Real-Time Comparative Quantitative PCR

Biomarker Insights, 2012
1p/19q (1p and/or 19q) deletions are prognostic factors in oligodendroglial tumors (OT) and predict better survival after both chemotherapy and radiotherapy. While studying 1p/19q status as a potential variable within multivariate prognosis models for OT,
Abhishek Chaturbedi, Liping Yu, Mark E Linskey, Yi-hong Zhou   +3 more
doaj   +2 more sources

Transcriptional Changes in Response to Arbuscular Mycorrhiza Development in the Model Plant Medicago truncatula

Molecular Plant-Microbe Interactions, 2003
Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant.
Anne Wulf, Katja Manthey, Jasmin Doll, Andreas M. Perlick, Burkhard Linke, Thomas Bekel, Folker Meyer, Philipp Franken, Helge Küster, Franziska Krajinski   +9 more
doaj   +1 more source