Evaluation of RNA extraction methods in rice and their application in expression analysis of resistance genes against Magnaporthe oryzae [PDF]
Biotechnology & Biotechnological Equipment, 2017 Extraction of RNA of high quality and integrity is essential for gene expression studies and all downstream RNA-based techniques. The leaves of 16 merit Malaysian rice varieties were used to isolate total RNA using five different methods.
Parisa Azizi +7 more
doaj +3 more sources
Comparison of subgingival bacterial sampling with oral lavage for detection and quantification of periodontal pathogens by real-time polymerase chain reaction [PDF]
, 2007 Background: Saliva has been studied for the presence of subgingival pathogens in periodontitis patients. With the anaerobic culture technique, the discrepancy between salivary recovery and subgingival presence has been significant, which makes this ...
Boutaga, Khalil +3 more
core +5 more sources
Design and evaluation of 16S rRNA sequence based oligonucleotide probes for the detection and quantification of Comamonas testosteroni in mixed microbial communities [PDF]
, 2006 Background The β-proteobacterial species Comamonas testosteroni is capable of biotransformation and also biodegradation of a range of chemical compounds and thus potentially useful in chemical manufacturing and bioremediation.
Bathe, Stephan, Hausner, Martina
core +2 more sources
Detection of Pork-Derived Ingredients Based on Real-time Quantitative PCR
Shipin gongye ke-ji, 2022 In order to accurately and reliably quantify pork-derived ingredients in meat products, single-copy gene (carcinoembryonic antigen-related cell adhesion molecule 2-like, CACA) in pig cell nucleus was screened by bioinformatics methods, with CACA gene as ...
Yao YANG +5 more
doaj +1 more source
Revista Brasileira de Zootecnia, 2020 To screen intestinal barrier genes associated with porcine epidemic diarrhea virus (PEDV) infection, in the present study we first detected PEDV-infected piglets ( Sus scrofa ) with intestinal damage using quantitative real-time PCR (qPCR) and ...
Xiaoyi Qi +5 more
doaj +1 more source
Involvement of Fenton chemistry in rice straw degradation by the lignocellulolytic bacterium Pantoea ananatis Sd-1 [PDF]
, 2016 Additional file 7: Table S2.
Bin Liu +12 more
core +6 more sources
A novel and universal method for microRNA RT-qPCR data normalization [PDF]
, 2009 Gene expression analysis of microRNA molecules is becoming increasingly important. In this study we assess the use of the mean expression value of all expressed microRNAs in a given sample as a normalization factor for microRNA real-time quantitative PCR
De Weer, An +6 more
core +2 more sources
Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR)
Medical Journal of Dr. D.Y. Patil Vidyapeeth, 2022 Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout.
Neeta Mishra +6 more
doaj +1 more source
Differential expression of cellulose synthase (CesA) gene transcripts in potato as revealed by QRT-PCR [PDF]
, 2009 Two transgenic potato lines, csr2–1 and csr4–8 that contained two different antisense cellulose synthase (CesA) genes, csr2 and csr4, respectively were crossed.
Jacobsen, E. +3 more
core +2 more sources
A quantitative real-time PCR method for absolute telomere length
BioTechniques, 2008 Telomere shortening is an important risk factor for cancer and accelerated aging. Here we describe the development of a simple and reproducible method to measure absolute telomere length. Based on Cawthon's quantitative real-time PCR (qRT-PCR) assay, our
Nathan J. O'Callaghan +3 more
doaj +1 more source