Results 11 to 20 of about 1,061,192 (206)

Real-Time PCR for mRNA Quantitation

open access: yesBioTechniques, 2005
(Uploaded by Plazi for the Bat Literature Project) Real-time PCR has become one of the most widely used methods of gene quantitation because it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, has little to no post-amplification processing, and is amenable to increasing sample throughput.
Marisa L. Wong, Juan F. Medrano
openaire   +3 more sources

Comparison of subgingival bacterial sampling with oral lavage for detection and quantification of periodontal pathogens by real-time polymerase chain reaction [PDF]

open access: yes, 2007
Background: Saliva has been studied for the presence of subgingival pathogens in periodontitis patients. With the anaerobic culture technique, the discrepancy between salivary recovery and subgingival presence has been significant, which makes this ...
Boutaga, Khalil   +3 more
core   +5 more sources

Design and evaluation of 16S rRNA sequence based oligonucleotide probes for the detection and quantification of Comamonas testosteroni in mixed microbial communities [PDF]

open access: yes, 2006
Background The β-proteobacterial species Comamonas testosteroni is capable of biotransformation and also biodegradation of a range of chemical compounds and thus potentially useful in chemical manufacturing and bioremediation.
Bathe, Stephan, Hausner, Martina
core   +1 more source

Quantitative Real-Time PCR

open access: yes, 2006
Changes in mRNA expression levels occur during physiological and pathological processes in the cardiovascular system. An increase inDNAtranscription results in increasedmRNAlevels and will subsequently result in increased protein levels that regulate processes inside and outside the cell.
Sluijter, J. P. G.   +2 more
openaire   +2 more sources

Differential gene expression in response to cold stress in Lepidium apetalum during seedling emergence

open access: yesBiologia Plantarum, 2012
Germination of Lepidium apetalum Wild. seeds is invariably arrested by cold stress. cDNA-amplified fragment length polymorphism (AFLP) technique was used to isolate genes relevant to chilling stress (4 °C) during seedling emergence. 43 transcript-derived
H. X. Zhao, Q. Li, G. Li, Y. Du
doaj   +1 more source

Detection of Salmonella Typhi in Bile by Quantitative Real-Time PCR

open access: yesMicrobiology Spectrum, 2022
In countries where the incidence of typhoid fever is high, fecal material from short-term carriers of Salmonella Typhi contaminates inadequately treated water supplies.
Ellen E. Higginson   +9 more
doaj   +1 more source

Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene

open access: yesMicromachines, 2020
Real-time polymerase chain reaction (PCR) is the standard for nucleic acid detection and plays an important role in many fields. A new chip design is proposed in this study to avoid the use of expensive instruments for hydrophobic treatment of the ...
Junru An   +4 more
doaj   +1 more source

Involvement of Fenton chemistry in rice straw degradation by the lignocellulolytic bacterium Pantoea ananatis Sd-1 [PDF]

open access: yes, 2016
Additional file 7: Table S2.
Bin Liu   +12 more
core   +6 more sources

Detection of Pork-Derived Ingredients Based on Real-time Quantitative PCR

open access: yesShipin gongye ke-ji, 2022
In order to accurately and reliably quantify pork-derived ingredients in meat products, single-copy gene (carcinoembryonic antigen-related cell adhesion molecule 2-like, CACA) in pig cell nucleus was screened by bioinformatics methods, with CACA gene as ...
Yao YANG   +5 more
doaj   +1 more source

Preliminary screening of intestinal barrier genes associated with porcine epidemic diarrhea virus infection in pigs

open access: yesRevista Brasileira de Zootecnia, 2020
To screen intestinal barrier genes associated with porcine epidemic diarrhea virus (PEDV) infection, in the present study we first detected PEDV-infected piglets ( Sus scrofa ) with intestinal damage using quantitative real-time PCR (qPCR) and ...
Xiaoyi Qi   +5 more
doaj   +1 more source

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