Results 21 to 30 of about 1,315,258 (335)
Micromachines, 2020 Real-time polymerase chain reaction (PCR) is the standard for nucleic acid detection and plays an important role in many fields. A new chip design is proposed in this study to avoid the use of expensive instruments for hydrophobic treatment of the ...
Junru An +4 more
doaj +1 more source
BMC Molecular Biology, 2010 The conclusions of thousands of peer-reviewed publications rely on data obtained using fluorescence-based quantitative real-time PCR technology. However, the inadequate reporting of experimental detail, combined with the frequent use of flawed protocols ...
S. Bustin +7 more
semanticscholar +1 more source
Exogenous Reference RNA for Normalization of Real-Time Quantitative PCR
BioTechniques, 2003 We have utilized an in vitro transcribed 3′ mRNA fragment of the plant gene ribulose bisphosphate carboxylase (RuBisCO) as an exogenous standard for normalization of quantitative PCR data. Both K562 cells and primary erythroid CD34+ progenitor cells were
Reginald D. Smith +3 more
doaj +1 more source
Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
Plant Methods, 2019 A suitable reference gene is an important prerequisite for guarantying accurate and reliable results in quantitative real-time PCR (qRT-PCR) analyses. However, there is no absolute universality in reference genes among different species.
Canbin Chen +10 more
semanticscholar +1 more source
Improving statistical inference on pathogen densities estimated by quantitative molecular methods: malaria gametocytaemia as a case study [PDF]
, 2015 BACKGROUND: Quantitative molecular methods (QMMs) such as quantitative real-time polymerase chain reaction (q-PCR), reverse-transcriptase PCR (qRT-PCR) and quantitative nucleic acid sequence-based amplification (QT-NASBA) are increasingly used to ...
A Gelman +56 more
core +4 more sources
Microorganisms, 2023 Equine piroplasmosis (EP) is a parasitic disease caused by Theileria equi (T. equi), Babesia caballi (B. caballi) and Theileria haneyi (T. haneyi). This disease is considered to be reportable by the World Organization for Animal Health (WOAH).
Bingqian Zhou +6 more
doaj +1 more source
Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques [PDF]
, 2019 Background: Tomato brown rugose fruit virus (ToBRFV) is a highly infectious tobamovirus that causes severe disease in tomato (Solanum lycopersicum L.) crops.
Alfaro-Fernandez A. +5 more
core +1 more source
FungiQuant: A broad-coverage fungal quantitative real-time PCR assay
BMC Microbiology, 2012 Background Fungal load quantification is a critical component of fungal community analyses. Limitation of current approaches for quantifying the fungal component in the human microbiome suggests the need for new broad-coverage techniques.
Liu Cindy M +11 more
doaj +1 more source
PLoS ONE, 2018 Gene expression analysis by quantitative PCR in skeletal muscle is routine in exercise studies. The reproducibility and reliability of the data fundamentally depend on how the experiments are performed and interpreted. Despite the popularity of the assay,
J. Kuang +4 more
semanticscholar +1 more source
Quantitative detection of soybean in meat products by a TaqMan real-time PCR assay [PDF]
, 2014 In the present work, we propose a normalised real-time quantitative PCR assay to determine the addition of soybean to meat products. The method proved to be a powerful tool for the quantification of soybean protein (dry basis) in the range of 0.01% to 6%,
Amaral, Joana S. +3 more
core +1 more source