Results 31 to 40 of about 1,315,258 (335)

DETERMINAZIONI VIRALI QUANTITATIVE MEDIANTE “PCR REAL TIME” CON IL SISTEMA “ROTOR GENETM”

Microbiologia Medica, 2005
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M.G. Marin, P. Turconi, F. Poma, F. Calamani, P. Guarneri, L. Berardi, L. Lattuada   +6 more
doaj   +1 more source

Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR in Macrobrachium Nipponense

International Journal of Molecular Sciences, 2018
Quantitative real-time PCR (qPCR) is widely used in molecular biology, although the accuracy of the quantitative results is determined by the stability of the reference genes used.
Yuning Hu, H. Fu, H. Qiao, Shengming Sun, Wenyi Zhang, S. Jin, Sufei Jiang, Y. Gong, Y. Xiong, Yan Wu   +9 more
semanticscholar   +1 more source

Evaluation and comparison of SYBR Green I Real-Time PCR and TaqMan Real-Time PCR methods for quantitative assay of Listeria monocytogenes in nutrient broth and milk [PDF]

, 2012
Specific traditional plate count method and real-time PCR systems based on SYBR Green I and TaqMan technologies using a specific primer pair and probe for amplification of iap-gene were used for quantitative assay of Listeria monocytogenes in seven ...
Bassami, M R, Dadkhah, H, Hashemi, S, Hosseini, H, Karatzas, Kimon Andreas G., Khaksar, R, Shahraz, F   +6 more
core   +1 more source

The performance of semi-quantitative differential PCR is similar to that of real-time PCR for the detection of the MYCN gene in neuroblastomas

Brazilian Journal of Medical and Biological Research, 2009
Amplification of the MYCN gene in neuroblastomas is a potent biological marker of highly aggressive tumors, which are invariably fatal unless sound clinical management is applied. To determine the usefulness of semi-quantitative differential PCR (SQ-PCR)
A.C.M.F. Souza, D.R.V. Souza, S.S. Sanabani, R.R. Giorgi, I. Bendit   +4 more
doaj   +1 more source

Detection and quantification of Verticillium dahliae and V. longisporum by droplet digital PCR versus quantitative real-time PCR

Frontiers in Cellular and Infection Microbiology, 2022
Vascular wilt, caused by Verticillium dahliae and V. longisporum, limits the quality and yield of agricultural crops. Although quantitative real-time PCR (qPCR) has greatly improved the diagnosis of these two pathogens over traditional, time-consuming ...
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su   +7 more
doaj   +1 more source

Investigations into the utility of real-time PCR for the detection, quantitation and characterisation of clinically relevant viruses [PDF]

, 2003
The use of PCR as a tool for the diagnostic virology and viral research laboratories has greatly increased in recent years, however the use of conventional PCR and amplicon detection systems can be a complex and relatively slow process that increases the
Mackay, Ian M.
core   +1 more source

Evaluation of reference genes at different developmental stages for quantitative real-time PCR in Aedes aegypti

Scientific Reports, 2017
The mosquito Aedes aegypti (Ae. aegypti) is the most notorious vector of illness-causing viruses such as Dengue, Chikugunya, and Zika. Although numerous genetic expression studies utilizing quantitative real-time PCR (qPCR) have been conducted with ...
Najat Dzaki, Karima N. Ramli, Azali Azlan, I. Ishak, G. Azzam   +4 more
semanticscholar   +1 more source

The prognostic impact of EGFR, ErbB2 and MET gene amplification in human gastric carcinomas as measured by quantitative Real-Time PCR [PDF]

, 2015
Purpose: Identification of critical genes which play pivotal roles in controlling tumor growth and survival will establish the basis for developing therapeutic targets. In this study, we focused on frequencies of EGFR, ErbB2 and MET gene amplification in
Farazmandfar, T., Janbabai, G., Naghshvar, F., Oladi, Z., Taghvaei, T.   +4 more
core   +1 more source

Selection and validation of reference genes for gene expression studies in Klebsiella pneumoniae using Reverse Transcription Quantitative real-time PCR

Scientific Reports, 2018
For reliable results, Reverse Transcription Quantitative real-time Polymerase Chain Reaction (RT-qPCR) analyses depend on stably expressed reference genes for data normalization purposes.
A. E. I. Gomes, Leonardo P Stuchi, Nathália Maria Gonçalves Siqueira, J. Henrique, R. Vicentini, M. Ribeiro, M. Darrieux, L. Ferraz   +7 more
semanticscholar   +1 more source

Simulation of between repeat variability in real time PCR reactions [PDF]

, 2012
While many decisions rely on real time quantitative PCR (qPCR) analysis few attempts have hitherto been made to quantify bounds of precision accounting for the various sources of variation involved in the measurement process.
Goetghebeur, Els, Lievens, Antoon, Van Aelst, Stefan, Van den Bulcke, Marc   +3 more
core   +3 more sources