Results 21 to 30 of about 53,016 (292)
Crystallization of Zika NS5 RdRp v1 [PDF]
The main aim of this work was to identify small molecules that bind Zika NS5 RdRp (catalytic RNA-dependent RNA polymerase domain) through X-ray fragment-based screening. The Zika NS5 RDRP domain was cloned, expressed, purified, and crystallised. Suitable crystals for fragment screening were produced and optimised allowing an extensive fragment ...
anu.chandran not provided +2 more
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Conformational Changes in Motif D of RdRPs as Fidelity Determinant [PDF]
Structure, 2012 RNA-dependent RNA polymerases (RdRPs) are the central players in both transcription and viral genome replication. Using NMR spectroscopy, Yang and colleagues (in this issue of Structure) show that the conformational changes in the structural motif D of poliovirus RdRP correlate with the nature of the bound nucleotide (correct versus incorrect), with a ...
Núria Verdaguer, Cristina Ferrer‐Orta
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RdRp or RT, That is the Question [PDF]
Molecular Biology and Evolution, 2021 Abstract The RNA-dependent RNA polymerase (RdRp) of all known double-stranded RNA viruses is located within the viral particle and is responsible for the transcription and replication of the viral genome. Through an RT-PCR assay, we determined that purified virions, in vitro translated RdRp proteins, and purified recombinant RdRp ...
Mahtab Peyambari +2 more
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Snapshots of a Non-Canonical RdRP in Action [PDF]
Viruses, 2021 RNA viruses typically encode their own RNA-dependent RNA polymerase (RdRP) to ensure genome replication and transcription. The closed “right hand” architecture of RdRPs encircles seven conserved structural motifs (A to G) that regulate the polymerization activity.
Diego S. Ferrero +2 more
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Biophysical Chemistry, 2021 Despite all the efforts to treat COVID-19, no particular cure has been found for this virus. Since developing antiviral drugs is a time-consuming process, the most effective approach is to evaluate the approved and under investigation drugs using in silico methods. Among the different targets within the virus structure, as a vital component in the life
Alireza Poustforoosh +5 more
semanticscholar +3 more sources
The worldwide search for the new mutations in the RNA-directed RNA Polymerase domain of SARS-CoV-2
Macedonian Veterinary Review, 2021 Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is an RNA virus, responsible for the current pandemic outbreak. In total, 200 genomes of the SARS‐CoV‐2 strains from four host organisms have been analyzed.
Siarhei A. Dabravolski +1 more
doaj +1 more source
Repurposed drug molecules targeting NSP12 protein of SARS-CoV-2: An in-silico study
AIMS Molecular Science, 2023 The emergence of SARS-CoV-2 created a havoc worldwide, causing high morbidity, serious complications and mortality. The ORF1ab of SARS-CoV-2 has 16 non-structural proteins which are required for genome replication and transcription.
Bhawna Sharma +14 more
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Exploring RdRp–remdesivir interactions to screen RdRp inhibitors for the management of novel coronavirus 2019-nCoV [PDF]
SAR and QSAR in Environmental Research, 2020 A novel coronavirus recently identified in Wuhan, China (2019-nCoV) has resulted in an increasing number of patients globally, and has become a highly lethal pathogenic member of the coronavirus family affecting humans. 2019-nCoV has established itself as one of the most threatening pandemics that human beings have faced, and therefore analysis and ...
Singh, P.K., Pathania, S., Rawal, R.K.
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Медицинская иммунология, 2022 Rapid emergence and evolution of novel SARS-CoV-2 variants has raised concerns about their potential impact on efficiency of currently available vaccines.
Ali A. Dawood
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bioRxiv, 2022 Despite a rapid expansion in the number of known RNA viruses following the advent of metagenomic sequencing, the identification and annotation of highly divergent RNA viruses remains challenging, particularly from poorly characterized hosts and ...
Justine Charon +3 more
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