Results 51 to 60 of about 36,268 (188)

eEF1α1 bridges the interaction between ORF4 and RdRp.

, 2016
(A) CoIP of RdRp, ORF4, eEF1α1 and Tubulin β (TUBβ) in Huh7 cells. (B) Western of Huh7 cells expressing EGFP or heEF1α1 shRNA. “##”: nonspecific band. (C) Western of Huh7 cells expressing EGFP or hTubβ shRNA (Top).
Shalimar (538335), Vidya P. Nair (2585419), Karishma Bakshi (2585437), Milan Surjit (2585434), Abhilasha Madhvi (2585416), Akriti Srivastava (2585431), Baibaswata Nayak (292413), Saumya Anang (2585428), Chandru Subramani (2585425), Ranjith Kumar CT (2585422)   +9 more
core   +1 more source

Development of a method for evaluating the mRNA transcription activity of influenza virus RNA-dependent RNA polymerase through real-time reverse transcription polymerase chain reaction

Virology Journal, 2021
Background The development of an influenza RNA-dependent RNA polymerase (RdRp) inhibitor is required; therefore, a method for evaluating the activity of influenza RdRp needs to be developed.
Yuka Horio, Mototada Shichiri, Yuji Isegawa   +2 more
doaj   +1 more source

An Oral Antiviral Combination Against Severe COVID‐19 Enabled by the Synergistic Mechanisms of Action of Auranofin and Remdesivir

Advanced Science, EarlyView.
FDA‐approved anti‐inflammatory drug Auranofin inhibits SARS‐CoV‐2 replication by inhibiting viral Main protease activity and Spike processing required for viral entry. It shows strong synergy with nucleoside analogue Remdesivir, due to non‐overlapping mechanisms.
Oyahida Khatun, Rohan Narayan, Santhosh Kambaiah Nagaraj, Rishad Shiraz, Abinaya Kaliappan, Sumandeep Kaur, Ankur Singh, Siva Shanmugam Selvaraj, Gudepalya Renukaiah Rudramurthy, Chakenahalli Naveenkumar, Shridhar Narayanan, Radha Krishan Shandil, Shailly Tomar, Shashank Tripathi   +13 more
wiley   +1 more source

Beyond Charge: Field‐Effect Transistors in Multimodal Biosensing Platforms for Biomolecular Interaction Studies

Advanced Electronic Materials, EarlyView.
This review summarizes the developments in field‐effect transistor (FET)‐based dual‐mode sensor platforms and what can be learned from them. It identifies charge as a crucial factor in biomolecular interactions at interfaces, outlines the challenges of hybrid systems, and highlights their benefits.
Roger Hasler, Ciril Reiner‐Rozman, Christoph Kleber, Jakub Dostalek, Wolfgang Knoll   +4 more
wiley   +1 more source

Functional analysis of the cellular RNA-directed RNA polymerase (RdRP) in higher plants [PDF]

, 2003
The cellular RNA-directed RNA polymerase 2 (RdRP^2) full-length cDNA was successfully cloned and its authentic sequence has been deduced from the Lycopersicon esculentum cultivar Rentita (tomato).
Razvi, Fareha
core  

Evolutionary inferences from the analysis of mutation dynamics in the SARS-CoV-2 replication-transcription complex

Вавиловский журнал генетики и селекции
The SARS-CoV-2 virus continues to evolve and remains a significant public health threat, while the worldwide monitoring and sequencing of its genomic variants provide a unique opportunity to study its evolution and better understand its molecular ...
A. Yu. Palyanov, A. P. Devyaterikov, N. V. Palyanova, A. M. Shestopalov   +3 more
doaj   +1 more source

Chicken Pulmonary MicroRNAs Targeting the PB2 (Segment 1) of Avian Influenza Virus

Animal Research and One Health, EarlyView.
The PB2 segment of H5N1 is essential for replication and host adaptation. We screened 200 miRNAs and identified five (gga‐miR‐17‐3p, gga‐miR‐29a‐5p, gga‐miR‐1718, gga‐miR‐16c‐5p, and gga‐miR‐1744‐5p) using thermodynamic stability of heteroduplex, seed sequence complementarity, conservation, and accessibility, offering insights into host antiviral ...
Akanksha Choudhary, Nidhishree N. S, Rakesh Kumar Pundir, Muhasin Asaf V. N, Anamika Mishra, Ashwin Ashok Raut, Megha Agarwal, Amod Kumar   +7 more
wiley   +1 more source

RdRP mice display highly augmented antiviral defenses.

, 2015
(A) Heat map of differentially expressed genes in CNS tissue. Microarray data are presented as fold change (blue = 1-fold induction; purple = 8-fold upregulation; red = ≥15-fold upregulation).
Louisa M. Papke (746703), Laurie J. Zoecklein (833266), Allan J. Bieber (289677), Arthur E. Warrington (156029), William E. Matchett (833268), James H. Morrison (261909), Meghan M. Painter (746702), Jens O. Watzlawik (278985), Kari L. Turkowski (833269), Moses Rodriguez (156032), Eric M. Poeschla (261918), Tommy A. Rinkoski (833267)   +11 more
core   +1 more source

Recent Advances in Virus–Host Interactions, Antiviral Bioactive Compounds, and Breeding for Disease Resistance of Porcine Epidemic Diarrhea Virus

Animal Research and One Health, EarlyView.
Basic research on the PEDV infection cycle and virus–host interactions advances the development of anti‐PEDV drugs and disease‐resistant breeding and helps strengthen disease prevention and control while reducing economic losses in the swine industry.
Heyong Wu, Aosi Qi, Shuyu Zhong, Haozhen Du, Shimeng Guo, Hongsheng Ouyang, Hongming Yuan, Daxin Pang, Zicong Xie   +8 more
wiley   +1 more source

SARS-CoV-2 RdRp exhibits a kinase/phosphotransferase like activity.

, 2021
(A) The CoV-2 RdRp exhibits a kinase like activity akin to that of purified human Akt2. While mutations in the probable NiRAN active site (K73A, D218A) cause decline in the kinase like catalytic activity of the SARS-CoV-2 RdRp, mutating the RdRp ...
Mohammed Ahmad (9120880), Bichitra Kumar Biswal (11426826), Tanmay Majumdar (250305), Richard Mariadasse (8180901), Satish Tiwari (9120886), Deepsikha Kar (9120889), Jeyaraman Jeyakanthan (253364), Sayan Chakraborty (234378), Abhisek Dwivedy (9120883), Shailendra Mani (415181), Prafullakumar Tailor (704915), Sankar Bhattacharyya (369988), Sudipta Sonar (10861950)   +12 more
core   +1 more source