Results 111 to 120 of about 2,160 (134)

Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion.

Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion.
The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP(Sc)) has generated the potential for sensitive detection of prions. Here we developed a new PrP(Sc) amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of ≥1 fg of PrP(Sc) in diluted ...
openaire  

TDP-43 real-time quaking induced conversion reaction optimization and detection of seeding activity in CSF of amyotrophic lateral sclerosis and frontotemporal dementia patients. [PDF]

Brain Commun, 2020
Scialò C, Tran TH, Salzano G, Novi G, Caponnetto C, Chiò A, Calvo A, Canosa A, Moda F, Caroppo P, Silani V, Ticozzi N, Ratti A, Borroni B, Benussi L, Ghidoni R, Furlanis G, Manganotti P, Senigagliesi B, Parisse P, Brasselet R, Buratti E, Legname G.   +22 more
europepmc   +1 more source

Detection of prions in matching post-mortem skin and cerebrospinal fluid samples using second-generation real-time quaking-induced conversion assay. [PDF]

Sci Rep
Baranová S, Moško T, Brůžová M, Haldiman T, Kim C, Safar JG, Matěj R, Holada K.   +7 more
europepmc   +1 more source

Advancing surgical instrument safety: A screen of oxidative and alkaline prion decontaminants using real-time quaking-induced conversion with prion-coated steel beads as surgical instrument mimetic. [PDF]

PLoS One
Heinzer D, Avar M, Pfammatter M, Moos R, Schwarz P, Buhmann MT, Kuhn B, Mauerhofer S, Rosenberg U, Aguzzi A, Hornemann S.   +10 more
europepmc   +1 more source

Validation of a real-time quaking-induced conversion (RT-QuIC) assay protocol to detect chronic wasting disease using rectal mucosa of naturally infected, pre-clinical white-tailed deer (Odocoileus virginianus). [PDF]

PLoS One
Piel RB, Veneziano SE, Nicholson EM, Walsh DP, Lomax AD, Nichols TA, Seabury CM, Schneider DA.   +7 more
europepmc   +1 more source

Monitoring of chronic wasting disease using real-time quaking-induced conversion assay in Japan

Monitoring of chronic wasting disease using real-time quaking-induced conversion assay in Japan
There has been no report on Chronic wasting disease (CWD) cases in Japan to date; however, there is concern about the geographic spread of CWD. To clarify the CWD status in Japan, we conducted CWD monitoring using real-time quaking-induced conversion (RT-QuIC) assay which can detect the low level of CWD prions.
openaire  

Early and Pre-Clinical Detection of Prion Seeding Activity in Cerebrospinal Fluid of Goats using Real-Time Quaking-Induced Conversion Assay. [PDF]

Sci Rep, 2019
Favole A, Mazza M, Vallino Costassa E, D'Angelo A, Lombardi G, Marconi P, Crociara P, Berrone E, Gallo M, Palmitessa C, Orrù CD, Caughey B, Acutis PL, Caramelli M, Casalone C, Corona C.   +15 more
europepmc   +1 more source

Cerebrospinal fluid real-time quaking-induced conversion test for sporadic Creutzfeldt-Jakob disease in an 18-year-old woman: A case report. [PDF]

Medicine (Baltimore), 2017
Yao Y, Dong X, Guan H, Lu Q.
europepmc   +1 more source

Detection of chronic wasting disease prion seeding activity in deer and elk feces by real-time quaking-induced conversion. [PDF]

J Gen Virol, 2017
Henderson DM, Tennant JM, Haley NJ, Denkers ND, Mathiason CK, Hoover EA.   +5 more
europepmc   +1 more source

Are We Ready for Detecting α-Synuclein Prone to Aggregation in Patients? The Case of "Protein-Misfolding Cyclic Amplification" and "Real-Time Quaking-Induced Conversion" as Diagnostic Tools. [PDF]

Front Neurol, 2018
Paciotti S, Bellomo G, Gatticchi L, Parnetti L.   +3 more
europepmc   +1 more source