Results 91 to 100 of about 10,380 (154)

α-Synuclein conformers reveal link to clinical heterogeneity of α-synucleinopathies

Translational Neurodegeneration, 2023
α-Synucleinopathies, such as Parkinson’s disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, are a class of neurodegenerative diseases exhibiting intracellular inclusions of misfolded α-synuclein (αSyn), referred to as Lewy bodies ...
Matthias Schmitz, Niccolò Candelise, Sezgi Canaslan, Hermann C. Altmeppen, Jakob Matschke, Markus Glatzel, Neelam Younas, Saima Zafar, Peter Hermann, Inga Zerr   +9 more
doaj   +1 more source

Enhanced prion detection in biological samples by magnetic particle extraction and real-time quaking-induced conversion

Journal of General Virology, 2016
Prions have been demonstrated in body fluids and excreta using bioassay, but at levels too low for detection by conventional direct-detection assays. More rapid and sensitive detection of prions in these clinically accessible specimens would be valuable for diagnosis and investigations of transmission, environmental impact, and interventions.
Nathaniel D, Denkers, Davin M, Henderson, Candace K, Mathiason, Edward A, Hoover   +3 more
openaire   +2 more sources

Rapid end-point quantitation of prion seeding activity with sensitivity comparable to bioassays. [PDF]

PLoS Pathogens, 2010
A major problem for the effective diagnosis and management of prion diseases is the lack of rapid high-throughput assays to measure low levels of prions. Such measurements have typically required prolonged bioassays in animals.
Jason M Wilham, Christina D Orrú, Richard A Bessen, Ryuichiro Atarashi, Kazunori Sano, Brent Race, Kimberly D Meade-White, Lara M Taubner, Andrew Timmes, Byron Caughey   +9 more
doaj   +1 more source

Researching Rupture: Engaged and Ethical Research on Extreme Nature–Society Disruption

Antipode, Volume 58, Issue 3, May 2026.
Abstract Global escalation in social and environmental disruption raises crucial methodological and ethical questions for researchers working in impacted communities. Interpretive social science and humanities research can make visible the experiences of those living through socio‐ecological “rupture”.
Sango Mahanty, Carolyn M. Hendriks, Sarah Milne, Anthea Snowsill, Raven Cretney, Laura Davy   +5 more
wiley   +1 more source

Structural conservation of prion strain specificities in recombinant prion protein fibrils in real-time quaking-induced conversion [PDF]

Prion, 2015
A major unsolved issue of prion biology is the existence of multiple strains with distinct phenotypes and this strain phenomenon is postulated to be associated with the conformational diversity of the abnormal prion protein (PrP(Sc)). Real-time quaking-induced conversion (RT-QUIC) assay that uses Escherichia coli-derived recombinant prion protein (rPrP)
Sano, Kazunori, Atarashi, Ryuichiro, Nishida, Noriyuki   +2 more
openaire   +2 more sources

Bank vole prion protein extends the use of RT-QuIC assays to detect prions in a range of inherited prion diseases

Scientific Reports, 2021
The cerebrospinal fluid (CSF) real-time quaking-induced conversion assay (RT-QuIC) is an ultrasensitive prion amyloid seeding assay for diagnosis of sporadic Creutzfeldt–Jakob disease (CJD) but several prion strains remain unexplored or resistant to ...
Tze How Mok, Akin Nihat, Connie Luk, Danielle Sequeira, Mark Batchelor, Simon Mead, John Collinge, Graham S. Jackson   +7 more
doaj   +1 more source

Evidence for different seeding activities of misfolded tau in classical and rapidly progressive Alzheimer's disease

Brain Pathology, Volume 36, Issue 3, May 2026.
The study analyzed the seeding activity of misfolded tau protein in Alzheimer's disease and rapidly progressive Alzheimer's disease patients and provides evidence for the existence of different tau assemblies supported by differences in cellular toxicity and morphology of thioflavin T‐positive real‐time quaking‐induced conversion products.
Matthias Schmitz, Katrin Thüne, Anna‐Lisa Fischer, Peter Hermann, Maria Cramm, Leonie Sheila Kadel, Wiebke Möbius, Jakob Matschke, Tayyaba Saleem, Neelam Younas, Saima Zafar, Hermann Altmeppen, Markus Glatzel, Inga Zerr   +13 more
wiley   +1 more source

A new diagnostic test for Creutzfeldt-Jakob disease: Real-time quaking-induced conversion (RT-QuIC)

Canada Communicable Disease Report, 2015
Currently, definitive diagnoses of Creutzfeldt-Jakob disease (CJD) require an examination of the brains of deceased patients. In cooperation with an international consortium, Canada's National Microbiology Laboratory is performing a validation of a new test that can diagnose CJD in living patients.
D, Godal, Slr, Simon, K, Cheng, J D, Knox   +3 more
openaire   +2 more sources

Seed Amplification Assays for Parkinson's Disease: A Review of α‐Synuclein Assays in Body Fluids and Tissues

Journal of Neurochemistry, Volume 170, Issue 5, May 2026.
Background: Parkinson's disease (PD) diagnosis is hindered by late motor symptom onset and low specificity of clinical assessments. This systematic review and meta‐analysis evaluates α‐synuclein seed amplification assays (SAAs) for PD and related synucleinopathies. Main Results: SAAs, including real‐time quaking‐induced conversion (RT‐QuIC) and protein
Weijie Kong, Katsuya Satoh, Mika Inada Shimamura, Tetsuya Maeda, Kenta Takahashi, Masanori Kurihara, Atsushi Iwata   +6 more
wiley   +1 more source

Sensitive and specific detection of classical scrapie prions in the brains of goats by real-time quaking-induced conversion

Journal of General Virology, 2016
Real-time quaking-induced conversion (RT-QuIC) is a rapid, specific and highly sensitive prion seeding activity detection assay that uses recombinant prion protein (rPrPSen) to detect subinfectious levels of the abnormal isoforms of the prion protein (PrPSc). Although RT-QuIC has been successfully used to detect PrPSc in various tissues from humans and
Rohana P, Dassanayake, Christina D, Orrú, Andrew G, Hughson, Byron, Caughey, Telmo, Graça, Dongyue, Zhuang, Sally A, Madsen-Bouterse, Donald P, Knowles, David A, Schneider   +8 more
openaire   +2 more sources