Results 1 to 10 of about 993,329 (331)
Real-Time PCR Assay for Quantitative Mismatch Detection [PDF]
BioTechniques, 2003 We describe here a quantitative realtime PCR assay for the detection of single-base-pair differences that does not require fluorescently labeled gene-specific probes or complicated primer combinations.
L. Shively +5 more
doaj +4 more sources
Quantitative analysis of the dystrophin gene by real-time PCR [PDF]
Archives of Biological Sciences, 2012 Duchenne and Becker muscular dystrophy (DMD/BMD) are severe X-linked neuromuscular disorders caused by mutations in the dystrophin gene. Our aim was to optimize a quantitative real-time PCR method based on SYBR® Green I chemistry for routine ...
Maksimovic Nela +12 more
doaj +4 more sources
Detection of 1p19q Deletion by Real-Time Comparative Quantitative PCR [PDF]
Biomarker Insights, 2012 1p/19q (1p and/or 19q) deletions are prognostic factors in oligodendroglial tumors (OT) and predict better survival after both chemotherapy and radiotherapy. While studying 1p/19q status as a potential variable within multivariate prognosis models for OT,
Abhishek Chaturbedi +3 more
doaj +3 more sources
Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene [PDF]
Micromachines, 2020 Real-time polymerase chain reaction (PCR) is the standard for nucleic acid detection and plays an important role in many fields. A new chip design is proposed in this study to avoid the use of expensive instruments for hydrophobic treatment of the ...
Junru An +4 more
doaj +2 more sources
Evaluation of RNA extraction methods in rice and their application in expression analysis of resistance genes against Magnaporthe oryzae [PDF]
Biotechnology & Biotechnological Equipment, 2017 Extraction of RNA of high quality and integrity is essential for gene expression studies and all downstream RNA-based techniques. The leaves of 16 merit Malaysian rice varieties were used to isolate total RNA using five different methods.
Parisa Azizi +7 more
doaj +3 more sources
Gene Dosage by Quantitative Real-Time PCR
BioTechniques, 1999 J.-L. Boulay +5 more
doaj +3 more sources
Real time quantitative PCR. [PDF]
Genome Research, 1996 We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies.
C A, Heid +3 more
openaire +2 more sources
Fermentation, 2022 Rummeliibacillus sp. TYF-LIM-RU47, isolated from the fermentation substrate of grain vinegar, could produce acetoin using a variety of carbon sources, including pentose, hexose and lignocellulose.
Guoyang Feng +5 more
doaj +1 more source
Comparison of subgingival bacterial sampling with oral lavage for detection and quantification of periodontal pathogens by real-time polymerase chain reaction [PDF]
, 2007 Background: Saliva has been studied for the presence of subgingival pathogens in periodontitis patients. With the anaerobic culture technique, the discrepancy between salivary recovery and subgingival presence has been significant, which makes this ...
Boutaga, Khalil +3 more
core +5 more sources
Molecular Plant-Microbe Interactions, 2003 Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant.
Anne Wulf +9 more
doaj +1 more source