Results 11 to 20 of about 993,329 (331)
Detection of Salmonella Typhi in Bile by Quantitative Real-Time PCR
Microbiology Spectrum, 2022 In countries where the incidence of typhoid fever is high, fecal material from short-term carriers of Salmonella Typhi contaminates inadequately treated water supplies.
Ellen E. Higginson +9 more
doaj +1 more source
Real-Time PCR for mRNA Quantitation
BioTechniques, 2005 (Uploaded by Plazi for the Bat Literature Project) Real-time PCR has become one of the most widely used methods of gene quantitation because it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, has little to no post-amplification processing, and is amenable to increasing sample throughput.
Marisa L. Wong, Juan F. Medrano
openaire +3 more sources
Design and evaluation of 16S rRNA sequence based oligonucleotide probes for the detection and quantification of Comamonas testosteroni in mixed microbial communities [PDF]
, 2006 Background The β-proteobacterial species Comamonas testosteroni is capable of biotransformation and also biodegradation of a range of chemical compounds and thus potentially useful in chemical manufacturing and bioremediation.
Bathe, Stephan, Hausner, Martina
core +2 more sources
, 2006 Changes in mRNA expression levels occur during physiological and pathological processes in the cardiovascular system. An increase inDNAtranscription results in increasedmRNAlevels and will subsequently result in increased protein levels that regulate processes inside and outside the cell.
Sluijter, J. P. G. +2 more
openaire +2 more sources
Biologia Plantarum, 2012 Germination of Lepidium apetalum Wild. seeds is invariably arrested by cold stress. cDNA-amplified fragment length polymorphism (AFLP) technique was used to isolate genes relevant to chilling stress (4 °C) during seedling emergence. 43 transcript-derived
H. X. Zhao, Q. Li, G. Li, Y. Du
doaj +1 more source
Detection of Pork-Derived Ingredients Based on Real-time Quantitative PCR
Shipin gongye ke-ji, 2022 In order to accurately and reliably quantify pork-derived ingredients in meat products, single-copy gene (carcinoembryonic antigen-related cell adhesion molecule 2-like, CACA) in pig cell nucleus was screened by bioinformatics methods, with CACA gene as ...
Yao YANG +5 more
doaj +1 more source
Involvement of Fenton chemistry in rice straw degradation by the lignocellulolytic bacterium Pantoea ananatis Sd-1 [PDF]
, 2016 Additional file 7: Table S2.
Bin Liu +12 more
core +6 more sources
A novel and universal method for microRNA RT-qPCR data normalization [PDF]
, 2009 Gene expression analysis of microRNA molecules is becoming increasingly important. In this study we assess the use of the mean expression value of all expressed microRNAs in a given sample as a normalization factor for microRNA real-time quantitative PCR
De Weer, An +6 more
core +2 more sources
Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR)
Medical Journal of Dr. D.Y. Patil Vidyapeeth, 2022 Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout.
Neeta Mishra +6 more
doaj +1 more source
Quantitative real-time RT-PCR – a perspective [PDF]
Journal of Molecular Endocrinology, 2005 The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the nucleic acid amplification and detection steps into one homogeneous assay and obviates the need for gel electrophoresis to detect ...
S A, Bustin +3 more
openaire +2 more sources