Results 21 to 30 of about 1,064,076 (204)

Quantitative real-time RT-PCR – a perspective [PDF]

Journal of Molecular Endocrinology, 2005
The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the nucleic acid amplification and detection steps into one homogeneous assay and obviates the need for gel electrophoresis to detect ...
S A, Bustin, V, Benes, T, Nolan, M W, Pfaffl   +3 more
openaire   +2 more sources

A quantitative real-time PCR method for absolute telomere length

BioTechniques, 2008
Telomere shortening is an important risk factor for cancer and accelerated aging. Here we describe the development of a simple and reproducible method to measure absolute telomere length. Based on Cawthon's quantitative real-time PCR (qRT-PCR) assay, our
Nathan J. O'Callaghan, Varinderpal S. Dhillon, Philip Thomas, Michael Fenech   +3 more
doaj   +1 more source

Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR)

Medical Journal of Dr. D.Y. Patil Vidyapeeth, 2022
Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout.
Neeta Mishra, Chandrashekhar Raut, Neelam Prasad, J S Bhawalkar, Safia Farooqui, Shahzad Mirza, Veshal V Madan   +6 more
doaj   +1 more source

Differential expression of cellulose synthase (CesA) gene transcripts in potato as revealed by QRT-PCR [PDF]

, 2009
Two transgenic potato lines, csr2–1 and csr4–8 that contained two different antisense cellulose synthase (CesA) genes, csr2 and csr4, respectively were crossed.
Jacobsen, E., Olawole, O., Vincken, J.P., Visser, R.G.F.   +3 more
core   +2 more sources

Exogenous Reference RNA for Normalization of Real-Time Quantitative PCR

BioTechniques, 2003
We have utilized an in vitro transcribed 3′ mRNA fragment of the plant gene ribulose bisphosphate carboxylase (RuBisCO) as an exogenous standard for normalization of quantitative PCR data. Both K562 cells and primary erythroid CD34+ progenitor cells were
Reginald D. Smith, Benjamin Brown, Pranvera Ikonomi, Alan N. Schechter   +3 more
doaj   +1 more source

Improving statistical inference on pathogen densities estimated by quantitative molecular methods: malaria gametocytaemia as a case study [PDF]

, 2015
BACKGROUND: Quantitative molecular methods (QMMs) such as quantitative real-time polymerase chain reaction (q-PCR), reverse-transcriptase PCR (qRT-PCR) and quantitative nucleic acid sequence-based amplification (QT-NASBA) are increasingly used to ...
A Gelman, André Lin Ouédraogo, B Hoadley, C Eisenhart, C Fraser, C Osborne, CE McCulloch, Cornelus Hermsen, D Lunn, DJ Lunn, DJ Spiegelhalter, DL Massart, EC Fieller, F Ferre, FA Graybill, G Marsaglia, GK Shulka, HG Niesters, HS Mubarak El, J Jiang, J Wain, JF Cohen, JH McCulloch, JM Ruijter, JN Miller, JO Beer De, JS Murray, JS Yuan, K Danzer, K Khairnar, KK Lai, LC Okell, LJ Naszódi, M Plummer, M Sivaganesan, M Woodhead, Martin Walker, María-Gloria Basáñez, NM Ferguson, P Schneider, P Schneider, P Vankeerberghen, PF Mens, R Wampfler, S Nadarajah, SA Bustin, SA Bustin, SA Fiscus, T Nolan, T Ponnudurai, TD Schmittgen, Teun Bousema, Thomas S Churcher, TS Churcher, World Health Organization, Y Karlen, YW Tang   +56 more
core   +4 more sources

FungiQuant: A broad-coverage fungal quantitative real-time PCR assay

BMC Microbiology, 2012
Background Fungal load quantification is a critical component of fungal community analyses. Limitation of current approaches for quantifying the fungal component in the human microbiome suggests the need for new broad-coverage techniques.
Liu Cindy M, Kachur Sergey, Dwan Michael G, Abraham Alison G, Aziz Maliha, Hsueh Po-Ren, Huang Yu-Tsung, Busch Joseph D, Lamit Louis J, Gehring Catherine A, Keim Paul, Price Lance B   +11 more
doaj   +1 more source

DETERMINAZIONI VIRALI QUANTITATIVE MEDIANTE “PCR REAL TIME” CON IL SISTEMA “ROTOR GENETM”

Microbiologia Medica, 2005
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M.G. Marin, P. Turconi, F. Poma, F. Calamani, P. Guarneri, L. Berardi, L. Lattuada   +6 more
doaj   +1 more source

Development of a Real-Time Quantitative PCR Based on a TaqMan-MGB Probe for the Rapid Detection of Theileria haneyi

Microorganisms, 2023
Equine piroplasmosis (EP) is a parasitic disease caused by Theileria equi (T. equi), Babesia caballi (B. caballi) and Theileria haneyi (T. haneyi). This disease is considered to be reportable by the World Organization for Animal Health (WOAH).
Bingqian Zhou, Guangpu Yang, Zhe Hu, Kewei Chen, Wei Guo, Xiaojun Wang, Cheng Du   +6 more
doaj   +1 more source

Quantitative detection of soybean in meat products by a TaqMan real-time PCR assay [PDF]

, 2014
In the present work, we propose a normalised real-time quantitative PCR assay to determine the addition of soybean to meat products. The method proved to be a powerful tool for the quantification of soybean protein (dry basis) in the range of 0.01% to 6%,
Amaral, Joana S., Mafra, I., Oliveira, Beatriz, Soares, Sónia   +3 more
core   +1 more source