Results 171 to 180 of about 574,812 (244)

Evaluation of Fecal Sample Pooling for Real-Time RT-PCR Testing SARS-CoV-2 in Animals. [PDF]

Viruses
Leonardi-Cattolica A, Kayastha S, Miller M, Guag J, Tkachenko A, Lowe J, Allender M, Terio K, Wang L.   +8 more
europepmc   +1 more source

Chronic Hypoxia Disrupts Spermatogenesis Through ASXL2–EZH2–Mediated Microtubule Destabilization

Advanced Science, EarlyView.
This study reveals the mechanism by which chronic hypoxia impairs spermatogenesis via the ASXL2–EZH2 axis, hindering the transition of spermatids from round to elongated forms. Key findings reveal that under hypoxic conditions, downregulated ASXL2 expression reduces EZH2 binding to the CEP162 promoter, leading to decreased H3K27me3 modification and ...
Jun Yin, Mengjie Zhang, Wenying Liu, Wenlong Shen, Debao Li, Hongming Miao, Fang Deng, Gang Zhang, Yi Tian, Yi Zhang, Zhihu Zhao, Bing Ni   +11 more
wiley   +1 more source

Author Correction: Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR. [PDF]

Sci Rep
Fazzina M, Bergonzoni M, Massenzio F, Monti B, Frabetti F, Casadei R.   +5 more
europepmc   +1 more source

Bufalin Inhibits the PI3K/AKT Pathway by Targeting GTF3C4 to Impede Breast Cancer Progression

Advanced Science, EarlyView.
Mechanisms of Bufalin‐Mediated Intervention in Breast Cancer. ABSTRACT Breast cancer incidence is rising globally, presenting challenges such as treatment side effects and drug resistance. Bufalin is a bufadienolides compound with potential anti‐cancer effects. This study shows that bufalin inhibits malignant proliferation of MDA‐MB‐231 and MCF‐7 cells
Siyu Guo, Xiaodong Chen, Haojia Wang, Jiying Zhou, Peiying Lu, Jiangying Liu, Keyan Chai, Jingyuan Zhang, Siyun Yang, Shan Lu, Yifei Gao, Zhengsen Jin, Xiaoyu Tao, Zhongdong Hu, Qinglin Li, Jiarui Wu   +15 more
wiley   +1 more source

Clinical performance of Abbott ID NOW™ COVID-19 2.0 rapid molecular point-of-care test compared to three real-time RT-PCR assays. [PDF]

Microbiol Spectr
Iglesias-Ussel MD, Bowie A, Anderson JG, Li Y, Park LP, Cardona JF, Dennis P, Ebuh V, Geller SA, Jain M, McKenzie MM, Merchant-Borna K, Patel A, Siegel A, Strauss GS, Thoppil JJ, Weinberg AS, Woods CW.   +17 more
europepmc   +1 more source

Tailoring Vascular‐Immune Homeostasis via Manganese‐DNA Complex‐Armed Immunogenic Extracellular Vesicles for Pancreatic Cancer Immunotherapy

Advanced Science, EarlyView.
This study demonstrates that Mn2⁺–tumor DNA complexes encapsulated in dendritic cell (DC)– derived immunogenic extracellular vesicles (EVDC@Mn‐DNA) act as a DC‐specific cGAS– STING activator. EVDC@Mn‐DNA treatment enhances intratumoral DC activation, improves tumor vascular function, promotes CD8⁺ T cell activity, and suppresses pancreatic tumor growth,
Xue Jiang, Lihuan Shang, Xiaochun Chen, Xiangzhan Kong, Xiaojuan Wang, Linjia Jiang, Huiying Zhao, Yafeng Zhu, Cheng Huang, Shiyi Deng, Rui Zhang, Minghui Wang, Haoming Lin, Ping‐Pui Wong   +13 more
wiley   +1 more source

Use of a diagnostic Puumala virus real-time RT-PCR in an orthohantavirus endemic region in the Netherlands. [PDF]

Microbiol Spectr
Geeraedts F, Wevers M, Bosma F, Boer Md, Brinkman JN, Delsing C, GeurtsvanKessel C, Rockx B, van der Zanden A, Laverman GD.   +9 more
europepmc   +1 more source

Ovarian Matrisome Dynamics and αvβ3‐Mediated Regulation in Early Follicular Development

Advanced Science, EarlyView.
The matrisome undergoes dynamic remodeling during early follicular development. Integrin αvβ3 mediates matrisome signals, regulating primordial follicle activation/atresia and secondary follicle growth via Hippo/mTOR pathways, with conserved roles in human ovaries, offering therapeutic targets for ovarian disorders.
Tong Wu, Yanzhi Feng, Qingqing Zhu, Ting Jiang, Yueyue Gao, Kebing Nie, Xianan Tang, Ying Chen, Dianxing Hu, Xudan Yang, Shixuan Wang, Meng Wu, Jinjin Zhang   +12 more
wiley   +1 more source

ƩS COVID-19 is a rapid high throughput and sensitive one-step quadruplex real-time RT-PCR assay. [PDF]

Sci Rep
Kowitdamrong E, Anoma S, Loykaew T, Hansasuta P, Bhattarakosol P.   +4 more
europepmc   +1 more source

Quantitative Real-time RT-PCR: Application to Carcinogenesis.

Cancer genomics & proteomics
Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) has simplified and enhanced the quantification of gene expression. However, since no agreed standardizations are available, care must be exercised when designing experiments, including the choice of appropriate amplification primers, detection chemistry and the ...
openaire   +1 more source