Comparative Performance of Digital PCR and Real-Time RT-PCR in Respiratory Virus Diagnostics [PDF]
VirusesBackground: Respiratory viral infections pose a major global health burden, and molecular diagnostics such as Real-Time RT-PCR have revealed frequent co-infections. However, precise quantification of viral RNA remains challenging.
Irene Bianconi +5 more
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Enhanced rabies surveillance in roadkill specimens by real-time RT-PCR. [PDF]
PLoS Neglected Tropical DiseasesRoadkill specimens are an important source of samples for enhanced rabies surveillance (ERS) in areas where other methods of sample collection may not be practical.
Crystal M Gigante +8 more
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Heparinase treatment of RNA before quantitative real-time RT-PCR [PDF]
BioTechniques, 2003 Vol. 35, No. 6 (2003) BioTechniques 1141 the ABI PRISM® 7000 and the TaqMan Universal PCR Master Mix protocols (Applied Biosystems). After discovering that the RNA from the thecal cells had vascular endothelial growth factor (VEGF) amplification and that RNA from granulosa cells had no VEGF amplification, the 18S rRNA amplification (TaqMan Pre ...
Mary Lynn Johnson +4 more
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Application of Metagenomic Next-Generation Sequencing (mNGS) and real-time RT-PCR Using different nasopharyngeal swabs in Diagnosing SARS-CoV-2 Omicron [PDF]
, 2023 LI S, Zhang Y, Tong p, Yang w.
europepmc +2 more sources
Application of standard and molecular methods for the diagnosis of newcastle disease [PDF]
Archives of Biological Sciences, 2012 Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007 ...
Vidanović D. +6 more
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Sensitive detection of novel Indian isolate of BTV 21 using ns1 gene based real-time PCR assay [PDF]
Veterinary World, 2013 Aim: The study was conducted to develop ns1 gene based sensitive real-time RT-PCR assay for diagnosis of India isolates of bluetongue virus (BTV). Materials and Methods: The BTV serotype 21 isolate (KMNO7) was isolated from Andhra Pradesh and propagated ...
Gaya Prasad +5 more
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Quantitative real-time RT-PCR – a perspective [PDF]
Journal of Molecular Endocrinology, 2005 The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the nucleic acid amplification and detection steps into one homogeneous assay and obviates the need for gel electrophoresis to detect ...
S A, Bustin +3 more
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استفاده از Real time RT-PCR جهت شناسایی ویروس زبان آبی ایران [PDF]
Archives of Razi Institute, 2011 طی سال های 1388-1389 از روش real time RT-PCR و RT-PCRمعمولی جهت شناسایی ویروس زبان آبی در 310 گوسفند مشکوک به بیماری زبان آبی استفاده شد. قطعاتS1 (ژن پلی مراز) و S10 (ژن NS3 ) بعنوان ژنهای حراست شده در گروه سرمی زبان آبی به ترتیب توسط rRT-PCRوRT-PCR ...
عبدالحمید شوشتری +3 more
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Real-time RT-PCR diagnostics of virus causing COVID-19
Фармакоэкономика, 2020 Aim: the study was aimed to develop a reagent kit for the real-time RT-PCR diagnostics of virus causing COVID-19.Materials and Methods. Three target sites were chosen in the genome SARS-CoV-2.
E. V. Goncharova +6 more
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تشخیص سریع و دقیق ویروس تب برفکی با روش PCR Real time در نمونههای بالینی [PDF]
Archives of Razi Institute, 2012 طی سالهای 1390-1389، از روش PCR Real-time برای ردیابی RNA ویروس تب برفکی در 147 نمونه اپیتلیوم از دامهای مشکوک به بیماری تب برفکی که از کانونهای مختلف اخذ گردیده بود استفاده شد.
همایون مهروانی +3 more
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