Foods, 2021 Foodborne viruses such as norovirus and hepatitis A virus cause frequent outbreaks associated with the consumption of raw or undercooked oysters. Viral particles are bioaccumulated in the oyster’s digestive glands, making RNA extraction and RT-PCR ...
Daniel Plante +4 more
doaj +1 more source
A Step Forward in Molecular Diagnostics of Lyssaviruses – Results of a Ring Trial among European Laboratories [PDF]
, 2013 Rabies is a lethal and notifiable zoonotic disease for which diagnostics have to meet the highest standards. In recent years, an evolution was especially seen in molecular diagnostics with a wide variety of different detection methods published ...
A Orlowska +56 more
core +12 more sources
Molecular Plant-Microbe Interactions, 2003 Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant.
Anne Wulf +9 more
doaj +1 more source
Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR [PDF]
, 2017 Background Direct immuno-fluorescence test (IFAT) and multiplex real-time RT-PCR have been central to RSV diagnosis in Kilifi, Kenya. Recently, these two methods showed discrepancies with an increasing number of PCR undetectable RSV-B viruses ...
Agoti, Charles N. +8 more
core +2 more sources
Real-time RT-PCR normalisation; strategies and considerations [PDF]
Genes & Immunity, 2005 Real-time RT-PCR has become a common technique, no longer limited to specialist core facilities. It is in many cases the only method for measuring mRNA levels of vivo low copy number targets of interest for which alternative assays either do not exist or lack the required sensitivity.
J, Huggett +3 more
openaire +2 more sources
Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques [PDF]
, 2019 Background: Tomato brown rugose fruit virus (ToBRFV) is a highly infectious tobamovirus that causes severe disease in tomato (Solanum lycopersicum L.) crops.
Alfaro-Fernandez A. +5 more
core +1 more source
Real-Time Quantitative RT-PCR: Design, Calculations, and Statistics [PDF]
The Plant Cell, 2009 Two recent letters to the editor of The Plant Cell ([Gutierrez et al., 2008][1]; [Udvardi et al., 2008][2]) highlighted the importance of following correct experimental protocol in quantitative RT-PCR (qRT-PCR).
Rieu, I., Powers, S. J.
openaire +2 more sources
Memorias do Instituto Oswaldo Cruz, 2012 In Niterói, state of Rio de Janeiro, dengue virus type 4 (DENV-4) was isolated for the first time in March 2011. We analysed the laboratory findings of the first cases and evaluated the use of molecular techniques for the detection of DENV-4 in Aedes ...
Márcia Gonçalves de Castro +11 more
doaj +1 more source
Development of real-time PCR and hybridization methods for detection and identification of thermophilic Campylobacter spp. in pig faecal samples [PDF]
, 2005 Aims: To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. Methods and results: The specificity of a developed 5’nuclease rt-PCR for
Al Rashid S.T. +10 more
core +1 more source
On joint maximum-likelihood estimation of PCR efficiency and initial amount of target [PDF]
, 2007 We consider the problem of estimating unknown parameters of the real-time polymerase chain reaction (RTPCR) from noisy observations. The joint ML estimator of the RT-PCR efficiency and the initial number of DNA target molecules is derived.
Hassibi, A., Hassibi, B., Vikalo, H.
core +1 more source