Results 11 to 20 of about 512,365 (262)

Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance

Memorias do Instituto Oswaldo Cruz, 2012
In Niterói, state of Rio de Janeiro, dengue virus type 4 (DENV-4) was isolated for the first time in March 2011. We analysed the laboratory findings of the first cases and evaluated the use of molecular techniques for the detection of DENV-4 in Aedes ...
Márcia Gonçalves de Castro, Rita Maria Ribeiro Nogueira, Ana Maria Bispo de Filippis, Anielly Alves Ferreira, Monique da Rocha Queiroz Lima, Nieli Rodrigues da Costa Faria, Fernanda de Bruycker Nogueira, Jaqueline Bastos Santos Simões, Priscila Conrado Guerra Nunes, Simone Alves Sampaio, Ricardo Lourenço-de-Oliveira, Flávia Barreto dos Santos   +11 more
doaj   +1 more source

Lotus japonicus LjKUP Is Induced Late During Nodule Development and Encodes a Potassium Transporter of the Plasma Membrane

Molecular Plant-Microbe Interactions, 2004
The KUP family of potassium transporters in plants is large but poorly characterized. We isolated and characterized the first KUP transporter from a legume, LjKUP of Lotus japonicus.
Guilhem Desbrosses, Claudia Kopka, Thomas Ott, Michael K. Udvardi   +3 more
doaj   +1 more source

Evaluation of Trypanosoma cruzi parasitic load by real-time PCR and blood culture in long-term kidney transplant recipients

Journal of Infection in Developing Countries, 2021
Introduction: Acute Chagas disease involving reactivation can occur after organ transplant, and follow-up by direct parasitological or molecular methods is essential for monitoring the parasitic load in such patients. In contrast, there is a little data
Juliana Jesus Guimarães Ferreira, Eros Antonio de Almeida, Gláucia Elisete Barbosa Marcon, Rodrigo Gonçalves Lima, Mariane Barroso Pereira, Fernanda Ramos Gadelha, Marilda Mazzali, Luiz Cláudio Martins, Jamiro Silva Wanderley, Sandra Cecília Botelho Costa   +9 more
doaj   +1 more source

Development of IC-RT real-time PCR method for detection of Cowpea severe mosaic virus

浙江大学学报. 农业与生命科学版, 2010
A method of immuno-capture reverse transcript real-time (IC-RT real-time) PCR assay was developed for detection of Cow pea severe mosaic virus (CPSMV).
LI Bin, SU Han, WU Cui-ping, ZHOU Ming-hua, AN Yu-lin   +4 more
doaj   +1 more source

Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology [PDF]

Archives of Biological Sciences, 2008
Metallothioneins (MTs) are an extensive and diverse family of small cysteine-rich proteins with metal-binding ability that are involved in metal homeostasis and detoxification. Two cDNA clones of the MT3 type, differing in 3’ UTRs, were isolated from the
Majić Dragana B., Samardžić Jelena T., Milisavljević Mira Đ., Krstić A.M., Maksimović Vesna R.   +4 more
doaj   +1 more source

SAS programs for real-time RT-PCR having multiple independent samples

BioTechniques, 2004
Relative real-time reverse transcription PCR (RT-PCR) has become an important tool for quantifying changes in messenger RNA (mRNA) populations following differential development or stimulation of tissues or cells. However, the best methods for conducting
Peyton Cook, Chunxiao Fu, Morgen Hickey, Eun-Soo Han, Kenton S. Miller   +4 more
doaj   +1 more source

Inter-Laboratory Comparison of RT-PCR-Based Methods for the Detection of Tomato Brown Rugose Fruit Virus on Tomato

Pathogens, 2022
In 2020, a test performance study (TPS) for the specific detection of tomato brown rugose fruit virus (ToBRFV) was organized in the frame of the H2020 Valitest project. Since no validated tests were available, all the protocols reported in the literature
Marta Luigi, Ariana Manglli, Antonio Tiberini, Sabrina Bertin, Luca Ferretti, Anna Taglienti, Francesco Faggioli, Laura Tomassoli   +7 more
doaj   +1 more source

Comparison of Multiplex Real-Time Polymerase Chain Reaction Assays for Detection of Respiratory Viruses in Nasopharyngeal Specimens

Annals of Clinical Microbiology, 2019
Background: Respiratory tract infections are major public health threats, and the identification of their causative microbes helps clinicians to initiate timely and appropriate antimicrobial therapy and prevent the secondary spread of infection. The main
, Min Kyung Kim, Daewon Kim, Jung-Hyun Byun, Dongeun Yong   +4 more
doaj   +1 more source

Development of a One-Step Immunocapture Real-Time RT-PCR Assay for Detection of Tobacco Mosaic Virus in Soil

Sensors, 2012
Tobacco mosaic virus (TMV) causes significant losses in many economically important crops. Contaminated soils may play roles as reservoirs and sources of transmission for TMV.
Jin-Guang Yang, Feng-Long Wang, De-Xin Chen, Li-Li Shen, Yu-Mei Qian, Zhi-Yong Liang, Wen-Chang Zhou, Tai-He Yan   +7 more
doaj   +1 more source

Missed detections of influenza A(H1)pdm09 by real-time RT–PCR assay due to haemagglutinin sequence mutation, December 2017 to March 2018, northern Viet Nam

Western Pacific Surveillance and Response, 2019
Introduction: There are two methods of reverse transcription polymerase chain reaction (RT–PCR) that have been the common methods to detect influenza infections: conventional and real-time RT–PCR.
Hoang Vu Mai Phuong, Ung Thi Hong Trang, Nguyen Le Khanh Hang, Nguyen Thanh Thuy, Le Thi Thanh, Nguyen Vu Son, Nguyen Phuong Anh, Tran Thi Thu Huong, Vuong Duc Cuong, Le Quynh Mai   +9 more
doaj   +1 more source