Results 31 to 40 of about 871,108 (257)
SAS programs for real-time RT-PCR having multiple independent samples
BioTechniques, 2004 Relative real-time reverse transcription PCR (RT-PCR) has become an important tool for quantifying changes in messenger RNA (mRNA) populations following differential development or stimulation of tissues or cells. However, the best methods for conducting
Peyton Cook +4 more
doaj +1 more source
Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR [PDF]
, 2016 Real Time Polymerase Chain Reaction also known as quantitative PCR (q-PCR) is a widely used tool in microbial ecology to quantify gene abundances of taxonomic and functional groups in environmental samples.
McKew, Boyd A +3 more
core +2 more sources
Pathogens, 2022 In 2020, a test performance study (TPS) for the specific detection of tomato brown rugose fruit virus (ToBRFV) was organized in the frame of the H2020 Valitest project. Since no validated tests were available, all the protocols reported in the literature
Marta Luigi +7 more
doaj +1 more source
Annals of Clinical Microbiology, 2019 Background: Respiratory tract infections are major public health threats, and the identification of their causative microbes helps clinicians to initiate timely and appropriate antimicrobial therapy and prevent the secondary spread of infection. The main
+4 more
doaj +1 more source
Detection of dengue virus in saliva and urine by real time RT-PCR [PDF]
, 2010 Early diagnosis of dengue virus (DENV) infection is important for patient management and control of dengue outbreaks. The objective of this study was to analyze the usefulness of urine and saliva samples for early diagnosis of DENV infection by real time
Telma R Poloni +7 more
core +1 more source
Real-time quantification of microRNAs by stem-loop RT-PCR [PDF]
Nucleic Acids Research, 2005 A novel microRNA (miRNA) quantification method has been developed using stem-loop RT followed by TaqMan PCR analysis. Stem-loop RT primers are better than conventional ones in terms of RT efficiency and specificity. TaqMan miRNA assays are specific for mature miRNAs and discriminate among related miRNAs that differ by as little as one nucleotide ...
Chen, Caifu +12 more
openaire +2 more sources
Sensors, 2012 Tobacco mosaic virus (TMV) causes significant losses in many economically important crops. Contaminated soils may play roles as reservoirs and sources of transmission for TMV.
Jin-Guang Yang +7 more
doaj +1 more source
Influenza a H5N1 detection [PDF]
, 2005 We developed a sensitive and rapid real-time reverse transcription- polymerase chain reaction (RT-PCR) assay to detect influenza A H5N1 virus in clinical samples.
Cheng, PKC +4 more
core +1 more source
Western Pacific Surveillance and Response, 2019 Introduction: There are two methods of reverse transcription polymerase chain reaction (RT–PCR) that have been the common methods to detect influenza infections: conventional and real-time RT–PCR.
Hoang Vu Mai Phuong +9 more
doaj +1 more source
Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control [PDF]
, 2011 <p>The current study reports on a real-time reverse transcription polymerase chain reaction (real-time RT-PCR) ring trial for the detection of Classical swine fever virus (CSFV) genomic RNA undertaken by 10 European laboratories.
Beer, Martin +18 more
core +7 more sources