Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process [PDF]
, 2008 12 pages, 2 figures, 4 tables.-- PMID: 19102748 [PubMed].-- PMCID: PMC2629474.[Background] The elucidation of gene expression patterns leads to a better understanding of biological processes.
Marino Expósito-Rodríguez +3 more
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MODERN LABORATORY DIAGNOSTIC METHODS IN VETERINARY MEDICINE AND THE POSSIBILITY OF ITS APPLICATION
Archives of Veterinary Medicine, 2010 One of the greatest challenges of modern laboratory diagnosis is the selection of methods for fast, highly sensitive and specific detection of the infective agents.
Tamaš Petrović +5 more
doaj +1 more source
Molecular epidemiology of human rhinovirus infections in Kilifi, coastal Kenya [PDF]
, 2012 This study reports pediatric surveillance over 3 years for human rhinovirus (HRV) at the District Hospital of Kilifi, coastal Kenya. Nasopharyngeal samples were collected from children presenting at outpatient clinic with no signs of acute respiratory ...
Agoti, Charles N. +8 more
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Species-specific Real Time-PCR primers/probe systems to identify fish parasites of the genera Anisakis, Pseudoterranova and Hysterothylacium (Nematoda: Ascaridoidea) [PDF]
, 2018 Ascaridoid nematodes belonging to the genera Anisakis and Pseudoterranova are heteroxenous parasites, involving marine mammals as definitive hosts in their life-cycles, whereas crustaceans (krill), fish and squids acting as intermediate/paratenic hosts.
Colantoni, Alessandra +5 more
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Data in Brief, 2017 This data article contains data related to the research article entitled “Rapid detection of enterovirus and Coxsackievirus A10 by a TaqMan based duplex one-step real time RT-PCR assay” (Chen at al., 2017) [1]. Primers and probe sequence design are among
Jingfang Chen +6 more
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Virus Culture and Real-time RT-PCR in Identifying Influenza Viruses From Influenza-like Illness Cases in Indonesia 2007-2008 [PDF]
, 2011 Introduction: From the influenza-like illness (ILI) surveillance in Indonesia, we learned that there was disagreement between virus culture and reverse trancriptase polymerase chain reaction (RT-PCR).
Indalao, I. L. (Irene) +3 more
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Determination of RNA stability using reverse transcription real-time PCR
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2008 The aim of this study was to verify an effect of RNA storage in different laboratory conditions. Especially, this work was focused on the importance of using diethylpyrocarbonate (DEPC) treated water for storage of isolated RNA.
Karel Bílek +2 more
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Appling real time RT-PCR for bluetongue virus detection in Iran
Archives of Razi Institute, 2011 During 2009-10, real time RT-PCR and conventional RT-PCR techniques Used for detecting BTVs RNA in310 blood samples. For real time and gel based RT-PCR segment-1 and segment-10 selected as conservegenes to search any BTV strains. Using these methods, 58 (
Shoshtari, A. +3 more
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Revista da Sociedade Brasileira de Medicina Tropical, 2016 : INTRODUCTION: The genus Flavivirus includes several pathogenic species that cause severe illness in humans. Therefore, a rapid and accurate molecular method for diagnosis and surveillance of these viruses would be of great importance.
Marilia Farignoli Romeiro +7 more
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Clinical Evaluation of Three Commercial RT-PCR Kits for Routine COVID-19 Diagnosis
Pathogens, 2022 Amongst the multiple ways to diagnose coronavirus disease-2019 (COVID-19), reverse transcription polymerase chain reaction (RT-PCR) remains the reference gold standard, providing fast and accurate results.
Rifat Tasnim Juthi +3 more
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