Results 181 to 190 of about 6,320 (210)
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Biochemistry, 1990
We have constructed a novel recombinant C5a anaphylatoxin (C5a-N19) containing a 19-residue amino-terminal extension peptide, using a plasmid vector which secretes the nascent polypeptide to the Escherichia coli periplasmic space. C5a-N19 was purified from cell lysates by immunoaffinity chromatography using a monoclonal antibody which recognizes a ...
N P, Gerard, C, Gerard
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We have constructed a novel recombinant C5a anaphylatoxin (C5a-N19) containing a 19-residue amino-terminal extension peptide, using a plasmid vector which secretes the nascent polypeptide to the Escherichia coli periplasmic space. C5a-N19 was purified from cell lysates by immunoaffinity chromatography using a monoclonal antibody which recognizes a ...
N P, Gerard, C, Gerard
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Anaphylatoxin C5a modulation of an alpha-adrenergic receptor system in the rat hypothalamus
Journal of Neuroimmunology, 1985C5a anaphylatoxin injected via implanted cannulae into the perifornical region of the hypothalamus stimulated eating in sated rats. C5a also attenuated carbamyl choline-induced drinking, and carbamyl choline inhibited C5a-induced eating, a mutual inhibition characteristic of the adrenergic-cholinergic interactions at this site.
C A, Williams, N, Schupf, T E, Hugli
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Gene, 1997
Binding and effector domains of the human anaphylatoxin C5a have been determined by either site directed mutagenesis or synthetic peptide studies. However, the lack of specific selection methods, which allow direct investigation of C5a-C5a-receptor interaction made these studies laborious.
M, Hennecke +6 more
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Binding and effector domains of the human anaphylatoxin C5a have been determined by either site directed mutagenesis or synthetic peptide studies. However, the lack of specific selection methods, which allow direct investigation of C5a-C5a-receptor interaction made these studies laborious.
M, Hennecke +6 more
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Demonstration of a specific receptor for human C5a anaphylatoxin on murine macrophages. [PDF]
Journal of Experimental Medicine, 1982 Human C5a anaphylatoxin is known to be a potent mediator of the acute inflammatory response. It serves to trigger a wide variety of neutrophil responses after binding to a specific cellular receptor. We have now demonstrated that this bioactive glycopolypeptide is also bound to a specific receptor found on murine resident peritoneal macrophages ...
Chenoweth, D E +2 more
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Cellular receptors to the anaphylatoxins C3a and C5a.
Biochemical Society symposium, 1987A single component in the plasma membrane of human polymorphonuclear leukocytes (hPMN) has been identified as the binding site for C5a. Labelled human C5a can be cross-linked to a 48,000-Mr membrane component on the hPMN surface by using the bifunctional reagent ethylene glycol bis(succinimidyl succinate).
R, Huey +3 more
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Receptors for the anaphylatoxins C3a and C5a are expressed in human atherosclerotic coronary plaques
Atherosclerosis, 2007The anaphylatoxins C3a and C5a are potent chemotactic and pro-inflammatory peptides that are released during complement activation, and recent clinical work have suggested them a role in acute coronary events. Here we studied whether human coronary plaques express anaphylatoxin receptors C3aR and C5aR, i.e. whether they have the potential to respond to
Riina, Oksjoki +7 more
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Expression cloning of a receptor for C5a anaphylatoxin on differentiated HL-60 cells
Biochemistry, 1991A cDNA clone encoding the human C5a anaphylatoxin receptor has been isolated by expression cloning from a CDM8 expression library prepared from mRNA of human myeloid HL-60 cells differentiated to the granulocyte phenotype with dibutyryladenosine cyclic monophosphate.
F, Boulay +4 more
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Kinetics of anaphylatoxin C5a receptor expression during experimental allergic encephalomyelitis
Journal of Neuroimmunology, 1998In this study, we investigated the expression of the C5aR in spinal cords of Lewis rats with experimental allergic encephalomyelitis (EAE). Using in situ hybridization (ISH) we analyzed the kinetics of C5aR at different time points of EAE (preclinical stage, clinical peak, remission phase).
S, Nataf, N, Davoust, S R, Barnum
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Identification of a Receptor-Binding Region in the Core Segment of the Human Anaphylatoxin C5a
Journal of Medicinal Chemistry, 1994In order to identify regions of C5a that contribute to receptor binding and functional activity of the anaphylatoxin, a series of peptides was synthesized in which core segments have been attached to C-terminal segments via native peptidic or disulfide bonds. It has been found that residues Arg40 and Arg46 in the loop-3 region of the core induce a 1000-
I, Vlattas +9 more
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The Journal of Immunology, 1996
Abstract A computer program was designed to locate regions termed antisense homology boxes (AHB), i.e., 8-15 amino acid regions corresponding to complementary DNA strands. Two AHBs were found in C5a and eight within the C5a receptor.
L, Baranyi, W, Campbell, H, Okada
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Abstract A computer program was designed to locate regions termed antisense homology boxes (AHB), i.e., 8-15 amino acid regions corresponding to complementary DNA strands. Two AHBs were found in C5a and eight within the C5a receptor.
L, Baranyi, W, Campbell, H, Okada
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