Results 1 to 10 of about 537,066 (143)

Strategies and Considerations for Improving Recombinant Antibody Production and Quality in Chinese Hamster Ovary Cells. [PDF]

Front Bioeng Biotechnol, 2022
Recombinant antibodies are rapidly developing therapeutic agents; approximately 40 novel antibody molecules enter clinical trials each year, most of which are produced from Chinese hamster ovary (CHO) cells.
Zhang JH, Shan LL, Liang F, Du CY, Li JJ.   +4 more
europepmc   +2 more sources

Rapid-format recombinant antibody-based methods for the diagnosis of Clostridioides difficile infection: Recent advances and perspectives. [PDF]

Front Microbiol, 2022
Clostridioides difficile, the most common cause of nosocomial diarrhea, has been continuously reported as a worldwide problem in healthcare settings. Additionally, the emergence of hypervirulent strains of C.
Raeisi H, Azimirad M, Asadzadeh Aghdaei H, Yadegar A, Zali MR.   +4 more
europepmc   +2 more sources

Development of Recombinant Antibody by Yeast Surface Display Technology. [PDF]

Curr Res Pharmacol Drug Discov
Recombinant antibodies have emerged as powerful tools in various fields, including therapeutics, diagnostics, and research applications. The selection of high-affinity antibodies with desired specificity is a crucial step in the development of ...
Mustafa MI, Alzebair AA, Mohammed A.
europepmc   +2 more sources

Recombinant Antibodies against Mycolactone

Toxins, 2019
In the past, it has proved challenging to generate antibodies against mycolactone, the primary lipidic toxin A of Mycobacterium ulcerans causing Buruli ulcer, due to its immunosuppressive properties. Here we show that in vitro display, comprising both phage and yeast display, can be used to select antibodies recognizing mycolactone from a large human ...
Naranjo, Leslie, Ferrara, Fortunato, Blanchard, Nicolas, Demangel, Caroline, D’angelo, Sara, Erasmus, M. Frank, Teixera, Andre, Bradbury, Andrew R.M.   +7 more
openaire   +5 more sources

Advances in recombinant antibody manufacturing. [PDF]

Appl Microbiol Biotechnol, 2016
Since the first use of Chinese hamster ovary (CHO) cells for recombinant protein expression, production processes have steadily improved through numerous advances. In this review, we have highlighted several key milestones that have contributed to the success of CHO cells from the beginning of their use for monoclonal antibody (mAb) expression until ...
Kunert R, Reinhart D.
europepmc   +5 more sources

Novel single-chain antibodies against highly conserved epitopes in the hemagglutinin of influenza A viruses: Promising agents for universal therapies [PDF]

Iranian Journal of Basic Medical Sciences, 2021
Objective(s): Development of new antibodies with broad activity would provide anti-influenza prophylaxis and treatment. Human single-chain variable fragments (scFvs) are considered effective agents against viruses.
Samaneh Alizadeh, Setareh Moazen, Seyed Nooreddin Faraji, Afagh Moattari, Foroogh Nejatollahi   +4 more
doaj   +1 more source

Research Progress in Affinity Enhancement Strategies for Recombinant Antibodies against Food Hazards [PDF]

Shipin Kexue, 2023
As a new generation of genetically engineered antibodies, recombinant antibodies are widely used in immunodetection of food hazards due to their simple structure as well as easy expression and modification.
WANG Feng, XIE Xi, MA Lukai, WANG Qin, XIAO Gengsheng, SHEN Yudong, XU Zhenlin, WANG Hong
doaj   +1 more source

Demystified… recombinant antibodies [PDF]

Journal of Clinical Pathology, 2004
Recombinant antibodies are important tools for biomedical research and are increasingly being used as clinical diagnostic/therapeutic reagents. In this article, a background to humanised antibodies is given, together with details of the generation of antibody fragments—for example, single chain Fv fragments.
Smith, K.A., Nelson, Paul N., Warren, Phil, Astley, S.J., Murray, Paul G., Greenman, J.   +5 more
openaire   +3 more sources

Recombinant Antibody Technology [PDF]

ILAR Journal, 1995
36:801-803. Smith, D.B., and K.S. Johnson. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31-40. Snapper, CM., and J.J. Mond. 1993. Towards a comprehensive view of immunoglobulin class switching. Immunol. Today 14:15-17. Snorasse, T., B. Flamand, G. De Becker, H. Bazin, F.
Alexander E., Karn, Christopher W., Bell, Tina F., Chin   +2 more
openaire   +2 more sources

CellectSeq: In silico discovery of antibodies targeting integral membrane proteins combining in situ selections and next-generation sequencing

Communications Biology, 2021
Kelil, Gallo et al. develop an antibody discovery strategy, termed CellectSeq, for targeting integral membrane proteins on native cells. This strategy uses motif-based scoring and sequencing error-filtering algorithms to identify antibodies with high ...
Abdellali Kelil, Eugenio Gallo, Sunandan Banerjee, Jarrett J. Adams, Sachdev S. Sidhu   +4 more
doaj   +1 more source