Results 101 to 110 of about 528,821 (173)

An extraction-free, lyophilized one-pot RAA-CRISPR assay for point-of-care testing of Haemophilus influenzae

Journal of Clinical Microbiology
Timely and accurate diagnosis is essential for the effective treatment of Haemophilus influenzae (HI) infection. Notably, a rapid point‐of-care testing (POCT) method for the diagnosis of HI DNA is still lacking.
Yaling Cao, Junwen Wang, Zihao Fan, Ling Xu, Zhenzhen Pan, Yinkang Mo, Qiwen Yang, Jing Huang, Feng Ren   +8 more
doaj   +1 more source

Rapid detection of feline parvovirus using RAA-CRISPR/Cas12a-based lateral flow strip and fluorescence

Frontiers in Microbiology
Feline parvovirus (FPV) causes severe gastroenteritis and leukopenia in cats, with high morbidity and mortality, necessitating a rapid and effective antigen diagnostic test with high sensitivity and specificity. In this study, a diagnostic platform based
Han Chen, Hailing Zhang, Jie Guo, Xiangshu Meng, Mengfan Yao, Longbin He, Xiaoxuan Nie, Han Xu, Chao Liu, Jian Sun, Jian Sun, Fei Wang, Yuelong Sun, Zhong Jiang, Yanliang He, Jianlou Zhang, Jianke Wang   +16 more
doaj   +1 more source

Rapid detection of Pseudomonas aeruginosa by glycerol one-pot RAA/CRISPR-Cas12a method

Frontiers in Chemistry
Pseudomonas aeruginosa (PA), an opportunistic pathogen commonly responsible for hospital-acquired infections, poses significant threats to human health.
Lijian Wei, Lijian Wei, Lijian Wei, Lijian Wei, Lijian Wei, Lijian Wei, Shihua Luo, Shihua Luo, Shihua Luo, Shihua Luo, Shihua Luo, Weijie Zhou, Baoyan Ren, Baoyan Ren, Miao Li, Lina Liang, Lina Liang, Lina Liang, Lina Liang, Lina Liang, Xuebin Li, Xuebin Li, Xuebin Li, Xuebin Li, Guijiang Wei, Guijiang Wei, Guijiang Wei, Guijiang Wei, Guijiang Wei, Guijiang Wei   +29 more
doaj   +1 more source

Detection method for reverse transcription recombinase-aided amplification of avian influenza virus subtypes H5, H7, and H9

Background Avian influenza virus (AIV) not only causes huge economic losses to the poultry industry, but also threatens human health. Reverse transcription recombinase-aided amplification (RT-RAA) is a novel isothermal nucleic acid amplification ...
Zichuang Zhang, Xianghe Zhai, Zongshu Zhang, Chunguang Wang, Xi Chen, Tie Zhang, Wenjing Wang   +6 more
core   +1 more source

Development of a real-time recombinase-aided amplification assay for rapid and sensitive detection of Edwardsiella piscicida

Frontiers in Cellular and Infection Microbiology
Edwardsiella piscicida, a significant intracellular pathogen, is widely distributed in aquatic environments and causes systemic infection in various species.
Yuchen Dong, Dandan Zhou, Binzhe Zhang, Xiaoying Xu, Jian Zhang   +4 more
doaj   +1 more source

DataSheet_1_Development of a real-time recombinase-aided amplification assay for rapid and sensitive detection of Edwardsiella piscicida.pdf

Edwardsiella piscicida, a significant intracellular pathogen, is widely distributed in aquatic environments and causes systemic infection in various species.
Yuchen Dong (229617), Xiaoying Xu (491572), Binzhe Zhang (18266992), Dandan Zhou (1573039), Jian Zhang (1682)   +4 more
core   +1 more source

Establishment of a Simple, Sensitive, and Specific Salmonella Detection Method Based on Recombinase-Aided Amplification Combined with dsDNA-Specific Nucleases

Salmonella is a common foodborne pathogen that can cause food poisoning, posing a serious threat to human health. Therefore, quickly, sensitively, and accurately detecting Salmonella is crucial to ensuring food safety.
Changyu Zhou, Ming Yang, Boyan Guo, Changwei Lei, Qiang Xu, Hongning Wang, Yu Zhao   +6 more
core   +1 more source

A Novel Sensitive Recombinase-Aided Amplification Integrated Test Strip for Pseudomonas fluorescens in Milk via Dual Gene Probes

Pseudomonas fluorescens is the main spoilage bacterium in milk, and its proliferation is one of the factors leading to the deterioration of the quality of raw milk. In this study, a rapid detection system for P.
Guangying Zhang, Jingqin Ye, Ying Lu, Dongshu Wang, Lili Zhang   +4 more
core   +1 more source

Establishment of a Real-Time Fluorescence Isothermal Recombinase-Aided Amplification Method for the Detection of H9 Avian Influenza Virus

The H9 subtype of avian influenza virus (AIV) has been characterized by its rapid spread, wide range of prevalence, and continuous evolution in recent years, leading to an increasing ability for cross-species transmission.
Jiaqi Li, Huan Cui, Yuxin Zhang, Ligong Chen, Shishan Dong, Cheng Zhang, Zitong Yang, Yejin Yang, Mingda Zhang, Heng Wang   +9 more
core   +1 more source

Rapid screening of ten tobamoviruses based on reverse‐transcription recombinase‐aided amplification and lateral flow dipstick

BACKGROUND Tobamoviruses infect economically important crops in the Solanaceae and Cucurbitaceae families, causing substantial economic losses.
Siyuan Wang, Yongjiang Zhang, Zhenxing Zhao, Zheng Dong   +3 more
core   +1 more source