Results 81 to 90 of about 528,821 (173)

CRISPR‐Enhanced RAA‐SHERLOCK Assay for Point‐of‐Care Detection of Cyprinid Herpesvirus‐3: Development, Validation and Clinical Application

Journal of Fish Diseases, Volume 49, Issue 4, April 2026.
ABSTRACT Koi herpesvirus disease (KHVD), caused by Cyprinid herpesvirus‐3 (CyHV‐3), poses a significant threat to global aquaculture due to its high mortality rates and economic impact. Current diagnostic methods, such as PCR, are limited by equipment dependency and procedural complexity, hindering point‐of‐care (POC) applications.
Hatem Soliman, Naveed Akram, Mona Saleh
wiley   +1 more source

Rapid visual detection of hepatitis E virus combining reverse transcription recombinase-aided amplification with lateral flow dipstick and real-time fluorescence

Journal of Clinical Microbiology
Hepatitis E virus (HEV) is a globally prevalent zoonotic pathogen that is primarily spread through the fecal-oral route, such as by consuming undercooked or contaminated pork.
Bingyan Wei, Wenlong Wang, Zixuan Guo, Wenjiao Yin, Minheng Cheng, Yifei Yang, Yuewei Tian, Yaxin Sun, Tianlong Liu, Yanxin Hu, Ruiping She, Jijing Tian   +11 more
doaj   +1 more source

Diagnosis of Mucormycosis: Current Situation, Challenges and Future Prospects

Mycoses, Volume 69, Issue 4, April 2026.
ABSTRACT Mucormycosis, an aggressive fungal infection caused by members of the order Mucorales, progresses rapidly and is associated with high mortality, particularly in immunocompromised hosts such as patients with uncontrolled diabetes, transplant recipients or those with COVID‐19‐associated immunosuppression. Early diagnosis remains challenging with
Yixuan Qu, Yuling Xiao, Lina Liu, Junyan Qu   +3 more
wiley   +1 more source

Droplet Digital CRISPR for Nucleic Acid Detection

Advanced Science, Volume 13, Issue 14, 9 March 2026.
This review outlines recent advances in droplet digital CRISPR technology for nucleic acid detection, combining CRISPR specificity with droplet digital‐based absolute quantification. It summarizes core principles, amplification‐assisted and amplification‐free strategies, and representative DNA and RNA biomarker applications.
Yang Zhang, Roy S. K. Walker, Anwar Sunna, Tracie J. Barber, Ming Li   +4 more
wiley   +1 more source

A Reverse Transcription Recombinase-Aided Amplification Method for Rapid and Point-of-Care Detection of SARS-CoV-2, including Variants

, 2021
The worldwide pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its emergence of variants needs rapid and point-of-care testing methods for a broad diagnosis.
Junping Yu, Yakun Lou, Haixia Zhang, Huige Zhang, Fengyun Li, Ping He, Qiaosheng Pu, Dongyan Xiong   +7 more
core   +1 more source

Digital Point‐of‐Care Testing Device Based on Bioluminescent Pyrophosphate in Real‐Time and Recombinase‐aid Amplification

Advanced Intelligent Systems, Volume 8, Issue 3, March 2026.
A point‐of‐care testing method based on solvent‐responsive magnetic beads suitable for large‐area diagnosis and mutation screening in remote areas was developed to detect it by combining bioluminescenct pyrophosphate in real‐time technology and recombinase‐aid amplification technology.
Zhongjie Fei, Juan Bai, Liwei Xu, Siyi Wang, Pengfeng Xiao, Dongrui Zhou, Guanxiong Liu, Rongbin Wei, Ping Jiang   +8 more
wiley   +1 more source

Table_1_Rapid and Sensitive Detection of Vibrio vulnificus Using CRISPR/Cas12a Combined With a Recombinase-Aided Amplification Assay.DOC

, 2021
Vibrio vulnificus is an important zoonotic and aquatic pathogen and can cause vibriosis in humans and aquatic animals (especially farmed fish and shrimp species). Rapid and sensitive detection methods for V.
Yan Zhou (6523), Ziqin Lin (11589580), Laibao Zheng (11589586), Yongliang Lou (416743), Xianhui Huang (11589583), Xingxing Xiao (7339139), Jinfang Lu (8129316)   +6 more
core   +1 more source

Advances in Rapid Nucleic Acid Diagnostics for Hepatitis B and C Viruses: A Comprehensive Review

Reviews in Medical Virology, Volume 36, Issue 2, March 2026.
ABSTRACT Chronic viral hepatitis, predominantly caused by HBV and HCV infections, remains a major global health burden and a leading cause of cirrhosis, hepatocellular carcinoma (HCC), and liver‐related mortality. Achieving the World Health Organisation (WHO) target of diagnosing 90% of HBV and HCV infections by 2030 necessitates a fundamental ...
Hsin‐Ying Ho, Chia‐Yen Dai, I‐Jung Feng, Yen‐Hsu Chen, Ming‐Lung Yu, Che‐Hsin Lin, Ling‐Shan Yu   +6 more
wiley   +1 more source

Advances in Double‐Stranded DNA Targeting Technologies

Exploration, Volume 6, Issue 1, February 2026.
Double‐stranded DNA (dsDNA) plays a crucial role in genetic information storage and disease management, but its inherent stability limits access to internal bases. To overcome this challenge, various high‐specificity molecular targeting technologies have been developed to destabilize the DNA structure.
Zuhao Shen, Yiqun Liu, Yingjie Hao, Yifan Bo, Xiaochuan Dai, Shihui Wang, Tian Xia, Xin Su, Huiyu Liu   +8 more
wiley   +1 more source

Mosquito‐Borne Viruses of Clinical Significance

Health Science Reports, Volume 9, Issue 2, February 2026.
ABSTRACT Background Mosquito‐borne viruses represent a significant public health concern globally, with rising incidence rates leading to increased morbidity and mortality. This review aims to synthesize current knowledge regarding mosquito‐borne viruses, focusing on their geographic distribution, clinical impact, diagnostic challenges, treatment ...
Donath Damian
wiley   +1 more source