Results 91 to 100 of about 528,821 (173)

Development of a Real-Time Recombinase-Aided Amplification Method to Rapidly Detect Methicillin-Resistant Staphylococcus aureus

, 2022
Methicillin-resistant staphylococcus aureus (MRSA) is a major pathogen responsible for human hospital and community-onset diseases and severe invasive livestock infections. Rapid detection of MRSA is essential to control the spread of MRSA.
Zhao, Qi, Bai, Yuhui, Cunshuai Zhang, Cui, Mingquan, Zhang, Cunshuai, Mingquan Cui, Shixin Xu, Cheng, Min, Chunping Zhang, Wang, Hejia, Yuhui Bai, Min Cheng, Xu, Shixin, Ding, Xiaoyan, Xiaoyan Ding, Qi Zhao, Li, Ting, Zhang, Chunping, Hejia Wang, Ting Li   +19 more
core   +1 more source

One-Step Reverse-Transcription Recombinase-Aided Amplification CRISPR/Cas12a-Based Lateral Flow Assay for Fast Field Screening and Accurate Differentiation of Four Major Tobamoviruses Infecting Tomato and Pepper

, 2023
Several tobamoviruses cause substantial economic losses to tomato and pepper crops globally, especially the pepper mild mosaic virus (PMMoV), tomato brown rugose fruit virus (ToBRFV), tomato mosaic virus (ToMV), and tomato mottle mosaic virus (ToMMV).
Qixuan Fan (17326160), Rong Lei (4006232), Yongjiang Zhang (416167), Siyuan Wang (147000), Ruirui Kuang (17326163), Zhenxing Zhao (6279890), Zheng Dong (10827)   +6 more
core   +1 more source

Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection

BMC Infectious Diseases, 2020
Background Mycoplasma pneumoniae is one of the most common causative pathogens of community-acquired pneumonia (CAP), accounting for as many as 30–50% of CAP during peak years.
Guanhua Xue, Shaoli Li, Hanqing Zhao, Chao Yan, Yanling Feng, Jinghua Cui, Tingting Jiang, Jing Yuan   +7 more
doaj   +1 more source

Achieving precise dual detection: One-tube reverse transcription-recombinase aided amplification (RT-RAA) combined with lateral flow strip (LFS) assay for RNA and DNA target genes from pepper mild mottle virus and Colletotrichum species in crude plant samples

Talanta
Ensuring the detection sensitivity of both RNA-derived and DNA-derived target genes in a single reaction has posed a significant challenge for on-site detection of plant pathogens. This challenge was addressed by developing a one-tube dual RT-RAA assay combined with LFS for the rapid on-site detection of pepper mild mottle virus (PMMoV) and four ...
Yuhao Cao, Dankan Yan, Huijie Zhou, Kelei Han, Qionglian Wan, Jiejun Peng, Hongying Zheng, Lin Lin, Fei Yan, Xuemei Song   +9 more
openaire   +2 more sources

Rapid and Visual Detection of Type 2 Porcine Reproductive and Respiratory Syndrome Virus by Real-Time Fluorescence-Based Reverse Transcription Recombinase-Aided Amplification

, 2022
Porcine reproductive and respiratory syndrome (PRRS) is one of the most important diseases that has brought significant economic losses to the swine industry worldwide.
Huaichang Sun, Zhu, Shanyuan, Wu, Zhi, Zhang, Hua, Xue Ding, Chen, Yao, Wu, Zhijun, Guo, Changming, Lu, Huipeng, Wenlong Xia, Sun, Huaichang, Zhi Wu, Liu, Xiaoming, Ding, Xue, Huipeng Lu, Hua Zhang, Shupei Yu, Zhijun Wu, Xia, Wenlong, Xiaoming Liu, Jing Huang, Zhongjun Fan, Yao Chen, Huang, Jing, Changming Guo, Yu, Shupei, Shanyuan Zhu, Fan, Zhongjun   +27 more
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Development of nucleic acid-based RAA test strip assay for the rapid detection of foot-and-mouth disease virus

Frontiers in Veterinary Science
Foot-and-mouth disease virus (FMDV) causes blister-like lesions in animals, leading to significant economic losses in animal husbandry. Accurate and rapid detection of FMD is crucial for effective prevention and control.
Wenxian Chen, Xinni Niu, Weijun Zeng, Yiqi Fang, Zixiang Zhu, Lin Yi, Mingqiu Zhao, Hongxing Ding, Shuangqi Fan, Zhaoyao Li, Jinding Chen   +10 more
doaj   +1 more source

Research Note: Real-time fluorescence-based recombinase-aided amplification for rapid detection of Mycoplasma synoviae

Poultry Science
: Mycoplasma synoviae (MS) is an essential pathogenic mycoplasma in poultry worldwide, posing a serious threat to the poultry industry's health. Timely detection is imperative for early diagnosis, prevention, and control of MS infection.
Wenlong Xia, Shupei Yu, Jing Huang, Yanan Li, Pei Wang, Shujun Shen, Minsheng Feng, Pengcheng Fu, Huilin Guan, Zhongjun Fan   +9 more
doaj   +1 more source

A Lab-on-a-Tube Biosensor Combining Recombinase-Aided Amplification and CRISPR-Cas12a with Rotated Magnetic Extraction for Salmonella Detection

, 2023
Background: Foodborne pathogenic bacteria threaten worldwide public health, and simple bacterial detection methods are in urgent need. Here, we established a lab-on-a-tube biosensor for simple, rapid, sensitive, and specific detection of foodborne ...
Jing Yuan, Shangyi Wu, Ai Xu, Xinge Xi, Yanbin Li, Lei Wang, Xiqing Yue, Jianhan Lin   +7 more
core   +1 more source

Visualization of Sex Identification in Red‐Crowned Crane (Grus japonensis) via Recombinase‐Aided Amplification Combined With Pyrococcus furiosus Argonaute Assay

Ecology and Evolution
The red‐crowned crane (Grus japonensis), a Class I protected animal in China, inhabits Northeast Asia, including China, Russia, and Japan. As sex‐monomorphic birds, red‐crowned cranes cannot be directly distinguished between females and males through ...
Shenluan Tan, Tongtong Zhan, Fanwen Zeng, Xuanjiao Chen, Tanzipeng Chen, Li Li, Hengxi Wei, Shouquan Zhang, Kejing Zuo   +8 more
doaj   +1 more source

Based on CRISPR-Cas13a system, to establish a rapid visual detection method for avian influenza viruses

Frontiers in Veterinary Science
To rapidly, specifically, and sensitively detect avian influenza virus (AIV), this research established a visual detection method of recombinase-aided amplification (RAA) based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and ...
Zongshu Zhang, Chunguang Wang, Xi Chen, Zichuang Zhang, Guoqiang Shi, Xianghe Zhai, Tie Zhang   +6 more
doaj   +1 more source