Results 71 to 80 of about 528,821 (173)

CRISPR‐Enabled Point‐of‐Care Diagnostics for Early Cancer Detection

Aggregate, Volume 7, Issue 7, July 2026.
This review summarizes recent advances in CRISPR‐enabled point‐of‐care diagnostics for early cancer detection, covering Cas‐based recognition mechanisms, portable engineering platforms, and biomarker applications. It further highlights current challenges and future opportunities for translating sensitive, affordable, and accessible CRISPR diagnostics ...
Yuwei Pan, Qihang Xu, Carla Spatola Rossi, Zhugen Yang   +3 more
wiley   +1 more source

Rapid Detection of the Varicella-Zoster Virus Using a Recombinase-Aided Amplification-Lateral Flow System

, 2022
Varicella-zoster virus (VZV) is the etiological agent of varicella (chickenpox) and herpes zoster (shingles). VZV infections are ubiquitous and highly contagious, and diagnosis is mostly based on the assessment of signs and symptoms.
Nicolas Berthet, Gary Wong, Selekon, Benjamin, Bienes, Kathrina Mae, Ella Gonofio, Berthet, Nicolas, Emmanuel Nakoune, Mao, Lingjing, Lingjing Mao, Gonofio, Ella, Bienes, Kathrina, Mae, Wong, Gary, Benjamin Selekon, Nakoune, Emmanuel, Kathrina Mae Bienes   +14 more
core   +1 more source

Dye‐based recombinase‐aided amplification assay with enhanced sensitivity and specificity

iLABMED
Background Fluorescent recombinase‐aided amplification (RAA) assays are increasingly being used in the detection of a variety of pathogens and have the advantages of rapidity and simplicity and similar sensitivity and specificity, compared with real‐time
Zijin Zhao, Yanbo You, Shaowei Hua, Xinxin Shen, Lingjun Li, Xuejun Ma   +5 more
doaj   +1 more source

Research Note: Application of reverse-transcription recombinase-aided amplification-lateral flow dipstick method in the detection of infectious bursal disease virus

Poultry Science, 2023
: Infectious bursal disease (IBD) is a highly contagious viral disease caused by infectious bursal disease virus (IBDV) in chickens. The consequent immunosuppression and secondary infection affect the healthy development of chicken industry.
Chao Wang, Chunguang Wang, Zongshu Zhang, Xinyue Li, Tie Zhang   +4 more
doaj   +1 more source

Fast detection of duck circovirus by real-time fluorescence-based recombinase-aided amplification

Poultry Science, 2022
: Duck circovirus disease (DuCVD), as an immunosuppressive disease, is a threat to the poultry industry. In order to diagnose this disease quickly and accurately, a real-time fluorescence-based recombinase-aided amplification (RF-RAA) method was ...
Xinyue Li, Chunguang Wang, Zongshu Zhang, Chao Wang, Wenjing Wang, Ziyu Zhao, Jikai Li, Zihan Shang, Jiancun Lv, Tie Zhang   +9 more
doaj   +1 more source

CRISPR‐Cas13a/Cas12a Assisted Dual Portable and Visualized HDV and HBV Detection

Journal of Medical Virology, Volume 98, Issue 6, June 2026.
ABSTRACT Co‐infection with both hepatitis B virus (HBV) and hepatitis D virus (HDV) can aggravate the severity of the end‐stage liver disease and accelerate its progression. However, no combined diagnostic method for HDV and HBV nucleic acids exists. In this study, we have developed a highly sensitive and specific dual detection method for HDV RNA and ...
Yuan Tian, Yaling Cao, Zhenzhen Pan, Ling Xu, Zihao Fan, Yinkang Mo, Xianru Zhu, Xiangying Zhang, Hao Li, Shengtao Zeng, Feng Ren   +10 more
wiley   +1 more source

Critical insight into recombinase polymerase amplification technology

, 2022
Introduction Recombinase polymerase amplification (RPA) is a promising and emerging technology for rapidly amplifying target nucleic acid from minimally processed samples and through small portable instruments. RPA is suitable for point-of-care testing (
Munawar, Mustafa Ahmad, Mustafa Ahmad Munawar   +1 more
core   +1 more source

Research Progress of Molecular Biological Technology in Rapid Detection of Foodborne Pathogens

Shipin gongye ke-ji
Foodborne pathogens are one of the important factors affecting food safety. Efficient and rapid detection technology of foodborne pathogens is particularly important to ensure food safety.
Defu ZHANG, Le GAO, Ming ZHANG, Zhixin DU, Xinran LÜ, Xue BAI, Guoqing ZHANG, Xuepeng LI, Jianrong LI   +8 more
doaj   +1 more source

Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection [PDF]

, 2015
© 2015 Rosser et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in ...
Rollinson, D, A. Rosser, D. Rollinson, Webster, BL, Rosser, A, Forrest, M, M. Forrest, B L Webster   +7 more
core   +1 more source

A Fluorogenic Biosensor for Direct Detection of Vibrio vulnificus, a Climate Change Biomarker

MicrobiologyOpen, Volume 15, Issue 2, April 2026.
Nanoporous anodic alumina (S2) loaded with rhodamine B and capped with a specific oligonucleotide probe (O1) for the vvhA gene. In the presence of V. vulnificus genomic DNA, probe displacement opens the pores and releases the dye, generating a measurable fluorescent signal.
M. Nieves Aranda, Isabel Caballos, Alba López‐Palacios, Héctor Carmona‐Salido, Eva Sanjuan, Elena Aznar, Carmen Amaro, Ramón Martínez‐Máñez, Andy Hernández‐Montoto   +8 more
wiley   +1 more source