Recombinase polymerase amplification of forensic short tandem repeat loci [PDF]
Scientific ReportsShort tandem repeats (STRs) are highly polymorphic repetitive DNA sequences extensively used in forensic science for identification of individuals. STR genotyping is usually performed by capillary electrophoresis (CE) or next-generation sequencing (NGS ...
Sonja Škevin +6 more
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Rapid detection of Mycobacterium tuberculosis by recombinase polymerase amplification. [PDF]
PLoS ONE, 2014 Improved access to effective tests for diagnosing tuberculosis (TB) has been designated a public health priority by the World Health Organisation. In high burden TB countries nucleic acid based TB tests have been restricted to centralised laboratories ...
David S Boyle +9 more
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Recombinase Polymerase Amplification-Based Biosensors for Rapid Zoonoses Screening
International Journal of Nanomedicine, 2023 Xinrui Feng,1,2,* Yan Liu,1,* Yang Zhao,3 Zhe Sun,1,4 Ning Xu,5 Chen Zhao,1 Wei Xia4 1College of Public Health, Jilin Medical University, Jilin, 132013, People’s Republic of China; 2Medical College, Yanbian University, Yanji, 136200, People’s ...
Feng X +6 more
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Establishment of recombinase polymerase amplification detection method for Dactylobotrys graminicola [PDF]
Scientific ReportsHulless barley sheath rot is a spike disease caused by Dactylobotrys graminicola. In recent years, it has generally occurred in hulless barley growing areas in China, resulting in reduced hulless barley yields.
Haiqing Zhang +5 more
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A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens [PDF]
Shipin Kexue, 2023 With the improvement of people’s living standards, food safety has attracted more and more social attention. Organisms, especially microorganisms, are the most important factor that affects food safety.
WANG Shuai, YANG Yange, WU Zhanwen, LI Hongna, LI Tao, SUN Dongmei, YUAN Fei
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Applying a Linear Amplification Strategy to Recombinase Polymerase Amplification for Uniform DNA Library Amplification [PDF]
ACS Omega, 2019 Recombinase polymerase amplification (RPA) is an isothermal DNA amplification method with broad applications as a point-of-care test and in molecular biology techniques. Currently, most of the applications are focused on target-specific amplification. Because RPA has the advantage of amplifying DNA under isothermal conditions, we utilized RPA as a DNA ...
Jeewon Lee, Sunghoon Heo, Duhee Bang
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Rapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assay. [PDF]
PLoS Neglected Tropical Diseases, 2019 BackgroundAccess to an accurate diagnostic test for Buruli ulcer (BU) is a research priority according to the World Health Organization. Nucleic acid amplification of insertion sequence IS2404 by polymerase chain reaction (PCR) is the most sensitive and ...
Michael Frimpong +7 more
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One-pot recombinase polymerase amplification and CRISPR/Cas12a assay for Cryptococcus neoformans [PDF]
Applied Microbiology and BiotechnologyCry pto coc cus neoformans, an opportunistic fungal pathogen, can induce central nervous system infections, posing a life-threatening risk and imposing substantial global health challenges and economic burdens.
Zixin Li +5 more
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Rapid detection of hepatitis C virus using recombinase polymerase amplification.
PLoS ONE, 2022 Over 71 million people are infected with hepatitis C virus (HCV) worldwide, and approximately 400,000 global deaths result from complications of untreated chronic HCV.
Catherine T Chia +8 more
doaj +3 more sources
Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection. [PDF]
PLoS ONE, 2015 Over 2.5 billion people are exposed to the risk of contracting dengue fever (DF). Early diagnosis of DF helps to diminish its burden on public health. Real-time reverse transcription polymerase amplification assays (RT-PCR) are the standard method for ...
Ahmed Abd El Wahed +10 more
doaj +7 more sources