Recombinase polymerase amplification: Basics, applications and recent advances
TrAC Trends in Analytical Chemistry, 2018 Recombinase polymerase amplification (RPA) is a highly sensitive and selective isothermal amplification technique, operating at 37-42°C, with minimal sample preparation and capable of amplifying as low as 1-10 DNA target copies in less than 20 min. It has been used to amplify diverse targets, including RNA, miRNA, ssDNA and dsDNA from a wide variety of
Lobato, Ivan Magriñá +1 more
openaire +3 more sources
Frontiers in Cellular and Infection Microbiology, 2022 After the outbreak of SARS-CoV-2, nucleic acid testing quickly entered people’s lives. In addition to the polymerase chain reaction (PCR) which was commonly used in nucleic acid testing, isothermal amplification methods were also important nucleic acid ...
Meiying Tan +5 more
doaj +2 more sources
One-step detection of Chlamydia psittaci using recombinase polymerase amplification and CRISPR/Cas12b [PDF]
AMB ExpressThe development of rapid, sensitive, and user-friendly diagnostic methods for Chlamydia psittaci (C. psittaci) is critical for early diagnosis and effective control of infections.
Jingyi Jiang +9 more
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Vibration mixing for enhanced paper-based recombinase polymerase amplification. [PDF]
Lab ChipIsothermal nucleic acid amplification tests are a vital tool for point-of-care diagnostics. In this work, we significantly improve paper-based recombinase polymerase amplification of HIV-1 DNA and RNA by employing a vibration-based mixing platform.
Shimazu KN +3 more
europepmc +3 more sources
Multiplexing using isothermal Recombinase Polymerase Amplification (RPA). [PDF]
BioTechniques, 2017 doaj +2 more sources
Multiplexing using isothermal Recombinase Polymerase Amplification (RPA). [PDF]
BioTechniques, 2018 doaj +2 more sources
Development of Reverse Transcriptase Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick for Rapid Detection of Tilapia Lake Virus (TiLV): Pilot Study [PDF]
Veterinary SciencesTilapia Lake Virus (TiLV) is well known as a highly contagious viral infection in aquaculture, particularly affecting Tilapia worldwide. Until recently, various TiLV diagnostic methods have been used for rapid and accurate diagnostic procedures that are ...
Saralee Srivorakul +7 more
doaj +2 more sources
Rapid and Sensitive Detection of the Causal Agents of Postharvest Kiwifruit Rot, Botryosphaeria dothidea and Diaporthe eres, Using a Recombinase Polymerase Amplification Assay [PDF]
The Plant Pathology Journal, 2023 The occurrence of postharvest kiwifruit rot has caused great economic losses in major kiwifruit-producing countries. Several pathogens are involved in kiwifruit rot, notably Botryosphaeria dothidea, and Diaporthe species.
Gi-Gyeong Park +2 more
doaj +1 more source
Solvent engineering studies on recombinase polymerase amplification [PDF]
Journal of Bioscience and Bioengineering, 2021 Recombinase polymerase amplification (RPA) is a technique that is used to specifically amplify a target nucleic acid sequence. Unlike the polymerase chain reaction (PCR), RPA is performed at a constant temperature between 37 and 42°C. Therefore, it can be potentially used for the onsite detection of various pathogens when combined with DNA extraction ...
Kenji Kojima +9 more
openaire +2 more sources
Inactivation and inducible oncogenic mutation of p53 in gene targeted pigs. [PDF]
, 2012 Mutation of the tumor suppressor p53 plays a major role in human carcinogenesis. Here we describe gene-targeted porcine mesenchymal stem cells (MSCs) and live pigs carrying a latent TP53(R167H) mutant allele, orthologous to oncogenic human mutant TP53 ...
Durkovic, Marina +13 more
core +12 more sources