Results 21 to 30 of about 131,465 (263)
Frontiers in Bioengineering and Biotechnology, 2023 The rapid diagnosis of pathogenic infections plays a vital role in disease prevention, control, and public health safety. Recombinase-aided amplification (RAA) is an innovative isothermal nucleic acid amplification technology capable of fast DNA or RNA ...
Xiaoping Li +16 more
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Recombinase Polymerase Amplification for Diagnostic Applications [PDF]
Clinical Chemistry, 2016 Abstract BACKGROUND First introduced in 2006, recombinase polymerase amplification (RPA) has stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them just in the last 2 years.
Rana K, Daher +3 more
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Recombinase polymerase amplification in minimally buffered conditions
Biosensors and Bioelectronics, 2022 Application of recombinase polymerase amplification (RPA) for pH-based detection of DNA amplification has been investigated. Commercial RPA kits from TwistDx are modified to minimize their pH buffering capacity. Due to the RPA's unique biochemistry, removal of tris from the amplification kit is not enough to lower the buffering capacity of the RPA ...
Saurabh Tomar +2 more
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P423 Recombinase polymerase amplification for Mucormycosis
Medical Mycology, 2022 Abstract Poster session 3, September 23, 2022, 12:30 PM - 1:30 PM Objectives (1) To develop a Recombinase polymerase amplification (RPA)-based assay to diagnose mucormycosis.
Hallur, Vinaykumar +4 more
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Development of a recombinase polymerase amplification lateral flow assay for the detection of active Trypanosoma evansi infections [PDF]
, 2020 Author summary Neglected tropical diseases (NTDs) affecting humans and/or domestic animals severely impair the socio-economic development of endemic areas. One of these diseases, animal trypanosomosis, affects livestock and is caused by the parasites of
Goossens, Julie +5 more
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Critical insight into recombinase polymerase amplification technology
Expert Review of Molecular Diagnostics, 2022 Recombinase polymerase amplification (RPA) is a promising and emerging technology for rapidly amplifying target nucleic acid from minimally processed samples and through small portable instruments. RPA is suitable for point-of-care testing (POCT) and on-site field testing, and it is compatible with microfluidic devices.
openaire +5 more sources
Development of Recombinase Polymerase Amplification Combined with Lateral Flow Strips for Rapid Detection of Cowpea Mild Mottle Virus [PDF]
The Plant Pathology Journal, 2023 Cowpea mild mottle virus (CPMMV) is a global plant virus that poses a threat to the production and quality of legume crops. Early and accurate diagnosis is essential for effective managing CPMMV outbreaks.
Xinyang Wu +10 more
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Detection of Entamoeba histolytica by Recombinase Polymerase Amplification [PDF]
The American Society of Tropical Medicine and Hygiene, 2015 Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Traditional microscopy approaches are neither sensitive nor specific for Entamoeba histolytica. Antigen assays are more specific, but many cases are missed unless tested by molecular methods.
Nair, Gayatri +8 more
openaire +3 more sources
Evaluation of Isothermal Recombinase Polymerase Amplification Incubation Temperature Tolerance [PDF]
BioTechniques, 2016 doaj +2 more sources
Veterinary and Animal Science, 2023 Alveolar echinococcosis in slaughtered horses remains a public health issue. This study aimed to develop a Recombinase polymerase amplification (RPA) assay targeting the mitochondrial NADH dehydrogenase subunit 5 (Nad5) gene of Echinococcus ...
Tatsuro Hifumi +5 more
doaj +1 more source