Results 31 to 40 of about 131,465 (263)

A recombinase polymerase amplification–based assay for rapid detection of Chlamydia psittaci

Poultry Science, 2021
Chlamydia psittaci is a zoonotic agent of systemic wasting disease in birds and atypical pneumonia in mammalians including humans, constituting a public health risk. A rapid diagnostic assay would be beneficial in screening C. psittaci in the field.
Yanling Pang, Feng Cong, Xinheng Zhang, Hongxin Li, Yung-Fu Chang, Qingmei Xie, Wencheng Lin   +6 more
doaj   +1 more source

Application of recombinase polymerase amplification with lateral flow assay to pathogen point-of-care diagnosis. [PDF]

Front Cell Infect Microbiol
Since the outbreak of the new coronavirus, point-of-care diagnostics based on nucleic acid testing have become a requirement for the development of pathogen diagnostics, which require the ability to accurately, rapidly, and conveniently detect pathogens.
Zhao Y, Wei Y, Ye C, Cao J, Zhou X, Xie M, Qing J, Chen Z.   +7 more
europepmc   +2 more sources

Differential DNA accessibility to polymerase enables 30-minute phenotypic β-lactam antibiotic susceptibility testing of carbapenem-resistant Enterobacteriaceae [PDF]

, 2020
The rise in carbapenem-resistant Enterobacteriaceae (CRE) infections has created a global health emergency, underlining the critical need to develop faster diagnostics to treat swiftly and correctly.
Garner, Omai B., Ismagilov, Rustem F., Liaw, Eric J., Savela, Emily S., Schoepp, Nathan G., Winnett, Alexander   +5 more
core   +1 more source

PrimedRPA: primer design for recombinase polymerase amplification assays [PDF]

Bioinformatics, 2018
Abstract Summary Recombinase polymerase amplification (RPA), an isothermal nucleic acid amplification method, is enhancing our ability to detect a diverse array of pathogens, thereby assisting the diagnosis of infectious diseases and the detection of microorganisms in food and water.
Matthew Higgins, Matt Ravenhall, Daniel Ward, Jody Phelan, Amy Ibrahim, Matthew S Forrest, Taane G Clark, Susana Campino   +7 more
openaire   +3 more sources

Rapid detection of human coronavirus NL63 by isothermal reverse transcription recombinase polymerase amplification

Journal of Clinical Virology Plus, 2022
Background: Human coronaviruses are one of the leading causes for respiratory tract infections and for frequent primary care consultation. The human coronavirus NL63 (HCoV..µNL63) is one representative of the seasonal coronaviruses and capable of ...
Aline Dorendorf, Iris Bachmann, Martin Spiegel, Ahmed Abd El Wahed, Gregory Dame, Frank Hufert   +5 more
doaj   +1 more source

A Real-Time Recombinase Polymerase Amplification Method for Rapid Detection of Vibrio vulnificus in Seafood

Frontiers in Microbiology, 2020
As an important foodborne pathogen, Vibrio vulnificus gives a significant threat to food safety and public health. Rapid and accurate detection methods for V. vulnificus are required to control its spread.
Xiaohan Yang, Xue Zhang, Yu Wang, Hui Shen, Ge Jiang, Jingquan Dong, Panpan Zhao, Song Gao   +7 more
doaj   +1 more source

Development of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of enterocytozoon hepatopenaei (EHP)

Aquaculture Reports, 2021
The emerging microsporidian parasite Enterocytozoon hepatopenaei (EHP) causes retardation of shrimp growth, leading to significant financial losses in shrimp aquaculture.
Gen Li, Feng Cong, Weiyou Cai, Jinhui Li, Miaoli Wu, Li Xiao, Xiaoliang Hu, Weiwei Zeng, Dongsheng He   +8 more
doaj   +1 more source

Data for designing two isothermal amplification assays for the detection of root-infecting fungi on cool-season turfgrasses

Data in Brief, 2018
Loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) are two rapid isothermal amplification methods for detecting three common fungal root pathogens of cool-season turfgrass: Gaeumannomyces avenae, Magnaporthiopsis
Brijesh B. Karakkat, Kurt Hockemeyer, Margot Franchett, Megan Olson, Cortney Mullenberg, Paul L. Koch   +5 more
doaj   +1 more source

Advancements in the synergy of isothermal amplification and CRISPR-cas technologies for pathogen detection

Frontiers in Bioengineering and Biotechnology, 2023
In the realm of pathogen detection, isothermal amplification technology has emerged as a swift, precise, and sensitive alternative to conventional PCR.
Xiaolei Mao, Minghui Xu, Shuyin Luo, Yi Yang, Jiaye Zhong, Jiawei Zhou, Huayan Fan, Xiaoping Li, Xiaoping Li, Zhi Chen   +9 more
doaj   +1 more source

Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection [PDF]

, 2015
© 2015 Rosser et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in ...
A Ahmed, A. Rosser, AM Emery, B L Webster, BB Obeng, C Escadafal, C Shiff, CH King, D Rollinson, D Rollinson, D Wichmann, D. Rollinson, EF Kjetland, F Amarir, JR Stothard, JR Stothard, JS McCarthy, K Krolov, L Cnops, M. Forrest, MG Cavalcanti, MJ Werf van der, N Lodh, N Nausch, NP Pai, NV Vinkeles Melchers, OP Akinwale, R Bergquist, S Kersting   +28 more
core   +1 more source