Rapid detection of Enterococcus and vancomycin resistance using recombinase polymerase amplification [PDF]
PeerJ, 2021 Vancomycin-resistant enterococci (VRE), especially Enterococcus faecium, have been a global concern, often causing serious healthcare-associated infections. We established a rapid approach for detecting E.
Pimchanok Panpru +8 more
doaj +2 more sources
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control [PDF]
, 2015 It was recently demonstrated that recombinaseﾠpolymeraseﾠamplification (RPA), an isothermal amplification platform for pathogen detection, may be used to quantify DNA sampleﾠconcentrationﾠusing aﾠstandard curveﾠIn this manuscript, a detailed protocol for
Crannell, Zachary A. +2 more
core +1 more source
Molecular diagnosis of protozoan parasites by Recombinase Polymerase Amplification [PDF]
Acta Tropica, 2018 Infections caused by protozoan parasites affect millions of people around the world. Traditionally, diagnosis was made by microscopy, which is insensitive and in some cases not specific. Molecular methods are highly sensitive and specific, but equipment costs and personnel training limit its availability only to specialized centers, usually far from ...
A, Castellanos-Gonzalez +3 more
openaire +2 more sources
The burden of congenital Chagas disease and implementation of molecular diagnostic tools in Latin America [PDF]
, 2018 It is estimated that between 8000 and 15 000 Trypanosoma cruzi infected babies are born every year to infected mothers in Chagas disease endemic countries. Currently, poor access to and performance of the current diagnostic algorithm, based on microscopy
Cruz, Israel +7 more
core +1 more source
Paradigm shift in the diagnosis of peste des petits ruminants: scoping review
Acta Veterinaria Scandinavica, 2020 Peste des petits ruminants virus causes a highly contagious disease, which poses enormous economic losses in domestic animals and threatens the conservation of wild herbivores.
Edson Kinimi +4 more
doaj +1 more source
Measures for diagnosing and treating infections by a novel coronavirus responsible for a pneumonia outbreak originating in Wuhan, China. [PDF]
, 2020 On 10 January 2020, a new coronavirus causing a pneumonia outbreak in Wuhan City in central China was denoted as 2019-nCoV by the World Health Organization (WHO).
Du, Lanying +4 more
core +1 more source
Equipment-free incubation of recombinase polymerase amplification reactions using body heat. [PDF]
PLoS ONE, 2014 The development of isothermal amplification platforms for nucleic acid detection has the potential to increase access to molecular diagnostics in low resource settings; however, simple, low-cost methods for heating samples are required to perform ...
Zachary Austin Crannell +2 more
doaj +1 more source
RNAase III-Type Enzyme Dicer Regulates Mitochondrial Fatty Acid Oxidative Metabolism in Cardiac Mesenchymal Stem Cells [PDF]
, 2019 Cardiac mesenchymal stem cells (C-MSC) play a key role in maintaining normal cardiac function under physiological and pathological conditions. Glycolysis and mitochondrial oxidative phosphorylation predominately account for energy production in C-MSC ...
Jin, Yue +5 more
core +1 more source
Nature Communications, 2022 Fast, inexpensive, and multiplexed detection of multiple nucleic acids is of great importance to human health, yet it still represents a significant challenge.
Zhichen Xu +9 more
semanticscholar +1 more source
Assessment of pan-Leishmania detection by recombinase polymerase amplification assay
Diagnostic Microbiology and Infectious Disease, 2023 The spread of vector habitats along with increasing human mobility can introduce atypical Leishmania species and hence can challenge existing diagnostic practices for rapid detection of active infection with species outside the narrow target range. Here we assessed the pan-Leishmania detection ability of isothermal recombinase polymerase amplification (
Chiheb Louizi +13 more
openaire +3 more sources