Results 51 to 60 of about 131,465 (263)

Molecular Detection of Feline Coronavirus Based on Recombinase Polymerase Amplification Assay

open access: yesPathogens, 2021
Feline coronavirus (FCoV) is endemic in cat populations worldwide. Persistently, subclinically infected cats play a significant role in spreading the infection.
Rea Maja Kobialka   +5 more
doaj   +1 more source

Rapid detection of Pseudomonas aeruginosa by recombinase polymerase amplification combined with CRISPR-Cas12a biosensing system

open access: yesFrontiers in Cellular and Infection Microbiology, 2023
Pseudomonas aeruginosa (P. aeruginosa) is an important bacterial pathogen involved in a wide range of infections and antimicrobial resistance. Rapid and reliable diagnostic methods are of vital important for early identification, treatment, and stop of P.
Shuang Liu   +11 more
semanticscholar   +1 more source

An enhanced isothermal amplification assay for viral detection

open access: yesNature Communications, 2020
Current state-of-the-art diagnostics for infectious diseases are sensitive but require extensive equipment. Here the authors develop an enhanced recombinase polymerase amplification reaction for SARS-CoV-2 that allows for inexpensive and rapid testing ...
Jason Qian   +10 more
doaj   +1 more source

Rapid detection of multiple resistance genes to last-resort antibiotics in Enterobacteriaceae pathogens by recombinase polymerase amplification combined with lateral flow dipstick

open access: yesFrontiers in Microbiology, 2023
The worrying emergence of multiple resistance genes to last-resort antibiotics in food animals and human populations throughout the food chain and relevant environments has been increasingly reported worldwide. Enterobacteriaceae pathogens are considered
Chenze Lu   +14 more
semanticscholar   +1 more source

Avoiding chromosome pathology when replication forks collide [PDF]

open access: yes, 2013
This is the author's accepted manuscript. The final published article is available from the link below. Copyright @ 2013 Macmillan Publishers Limited.Chromosome duplication normally initiates through the assembly of replication fork complexes at defined ...
Lloyd, RG   +3 more
core   +1 more source

A recombinase polymerase amplification assay for rapid detection of rabies virus

open access: yesScientific Reports, 2021
Rabies is a generally fatal encephalitis caused by a negative-sense single-stranded RNA lyssavirus transmitted to humans mainly from dog bite. Despite the recommendation by WHO and OIE to use the direct immunofluorescence test as standard method ...
Martin Faye   +6 more
doaj   +1 more source

Development of a CRISPR/Cas12a‐recombinase polymerase amplification assay for visual and highly specific identification of the Congo Basin and West African strains of mpox virus

open access: yesJournal of Medical Virology, 2023
Human mpox is a zoonotic disease, similar to smallpox, caused by the mpox virus, which is further subdivided into Congo Basin and West African clades with different pathogenicity.
Xinggui Yang   +8 more
semanticscholar   +1 more source

Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview

open access: yesZoonoses, 2022
With the advent of molecular technology, several isothermal techniques for rapid detection of zoonotic pathogens have been developed. Among them, recombinase polymerase amplification (RPA) is becoming an important technology for rapid, sensitive, and ...
Ruichen Lv   +4 more
doaj   +1 more source

Rapid detection of mcr-1 by recombinase polymerase amplification

open access: yesJournal of Medical Microbiology, 2018
The plasmid-mediated mcr-1 gene conferring colistin resistance has a strong ability to spread. The objective of this study was to establish a rapid and sensitive recombinase polymerase amplification (RPA) method for plasmid-mediated polymyxin-resistant gene mcr-1 detection.Using the reported sequence of the mcr-1 gene, we designed specific primers and ...
Jialiang, Xu   +4 more
openaire   +2 more sources

Europium Nanoparticle-Based Lateral Flow Strip Biosensors Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Five Zoonotic Foodborne Pathogens

open access: yesBiosensors, 2023
The five recognized zoonotic foodborne pathogens, namely, Listeria monocytogenes, Staphylococcus aureus, Streptococcus suis, Salmonella enterica and Escherichia coli O157:H7, pose a major threat to global health and social–economic development.
Bei Jin   +8 more
semanticscholar   +1 more source

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