Results 61 to 70 of about 131,465 (263)

Recent developments in nucleic acid identification using solid-phase enzymatic assays [PDF]

open access: yes, 2014
This review (containing 101 references) covers recent achievements in the development of new approaches for enzymatically assisted detection of nucleic acids on microarrays. We discuss molecular techniques including the polymerase chain reaction, reverse
Ellis, Amanda Vera, Khodakov, Dmitriy A
core   +1 more source

Development and application of crude sap-based recombinase polymerase amplification assay for the detection and occurrence of grapevine geminivirus A in Indian grapevine cultivars

open access: yesFrontiers in Plant Science, 2023
Geminiviruses are known to infect several fields and horticultural crops around the globe. Grapevine geminivirus A (GGVA) was reported in the United States in 2017, and since then, it has been reported in several countries.
G. Kishan   +6 more
semanticscholar   +1 more source

Clinical Validation of Two Recombinase-Based Isothermal Amplification Assays (RPA/RAA) for the Rapid Detection of African Swine Fever Virus

open access: yesFrontiers in Microbiology, 2020
African swine fever (ASF), caused by African swine fever virus (ASFV), is a devastating infectious disease of domestic pigs and wild boars, and has tremendous negative socioeconomic impact on the swine industry and food security worldwide.
Xiaoxu Fan   +17 more
doaj   +1 more source

A CRISPR/Cas12a-based portable platform for rapid detection of Leptosphaeria maculans in Brassica crops

open access: yesFrontiers in Plant Science, 2022
Establishing a portable diagnostic method for identifying plant pathogens is essential to prevent the spread of plant disease, especially in field and customs inspections. Leptosphaeria maculans (L.
Rong Lei   +10 more
doaj   +1 more source

Blocked recombinase polymerase amplification for mutation analysis of PIK3CA gene [PDF]

open access: yesAnalytical Biochemistry, 2018
A blocked recombinase polymerase amplification (blocked-RPA) approach has been developed for the enrichment of mutated templates in heterogeneous specimens as tumor tissues. This isothermal amplification technique opens alternative solutions for meeting the technological demand of physician office laboratories.
Sara Martorell   +3 more
openaire   +5 more sources

Centrifugal microfluidic-based multiplex recombinase polymerase amplification assay for rapid detection of SARS-CoV-2

open access: yesiScience, 2023
Summary The COVID-19 pandemic has spread worldwide, and rapid detection of the SARS-CoV-2 virus is crucial for infection surveillance and epidemic control. This study developed a centrifugal microfluidics-based multiplex reverse transcription recombinase
Ruoxu Li   +11 more
semanticscholar   +1 more source

Research Progress of Molecular Biological Technology in Rapid Detection of Foodborne Pathogens

open access: yesShipin gongye ke-ji
Foodborne pathogens are one of the important factors affecting food safety. Efficient and rapid detection technology of foodborne pathogens is particularly important to ensure food safety.
Defu ZHANG   +8 more
doaj   +1 more source

Amplification Techniques of Recombinase & Polymerase and their Application in Parasite Detection [PDF]

open access: yesE3S Web of Conferences, 2019
To study the application principles of recombinase polymerase amplification (RPA) and the specific situations of detecting parasites, the principles of RPA are analyzed to find the optimal temperature conditions, advantages, and disadvantages.
Niu Lili, Liu Hongli
doaj   +1 more source

Recombinase polymerase amplification assay for rapid detection of Monkeypox virus [PDF]

open access: yesDiagnostic Microbiology and Infectious Disease, 2019
In this study, a rapid method for the detection of Central and West Africa clades of Monkeypox virus (MPXV) using recombinase polymerase amplification (RPA) assay targeting the G2R gene was developed. MPXV, an Orthopoxvirus, is a zoonotic dsDNA virus, which is listed as a biothreat agent.
Davi, Saskia Dede   +12 more
openaire   +4 more sources

Development of Reverse Transcription Recombinase Polymerase Amplification (RT-RPA): A Methodology for Quick Diagnosis of Potato Leafroll Viral Disease in Potato

open access: yesInternational Journal of Molecular Sciences, 2023
Potatoes are developed vegetatively from tubers, and therefore potato virus transmission is always a possibility. The potato leafroll virus (PLRV) is a highly devastating virus of the genus Polerovirus and family Luteoviridae and is regarded as the ...
Ravinder Kumar   +10 more
semanticscholar   +1 more source

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