Results 41 to 50 of about 17,530 (137)

Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures

Nature Communications, 2021
RAD51 and DMC1 recombinases catalyse high-fidelity and mismatch tolerant recombination, processes that are indispensable for the maintenance of genomic integrity.
Shih-Chi Luo, Hsin-Yi Yeh, Wei-Hsuan Lan, Yi-Min Wu, Cheng-Han Yang, Hao-Yen Chang, Guan-Chin Su, Chia-Yi Lee, Wen-Jin Wu, Hung-Wen Li, Meng-Chiao Ho, Peter Chi, Ming-Daw Tsai   +12 more
doaj   +1 more source

Structures of RecBCD in complex with phage-encoded inhibitor proteins reveal distinctive strategies for evasion of a bacterial immunity hub

eLife, 2022
Following infection of bacterial cells, bacteriophage modulate double-stranded DNA break repair pathways to protect themselves from host immunity systems and prioritise their own recombinases.
Martin Wilkinson, Oliver J Wilkinson, Connie Feyerherm, Emma E Fletcher, Dale B Wigley, Mark S Dillingham   +5 more
doaj   +1 more source

Multi-color dSTORM microscopy in Hormad1-/- spermatocytes reveals alterations in meiotic recombination intermediates and synaptonemal complex structure.

PLoS Genetics, 2022
Recombinases RAD51 and its meiosis-specific paralog DMC1 accumulate on single-stranded DNA (ssDNA) of programmed DNA double strand breaks (DSBs) in meiosis.
Lieke Koornneef, Johan A Slotman, Esther Sleddens-Linkels, Wiggert A van Cappellen, Marco Barchi, Attila Tóth, Joost Gribnau, Adriaan B Houtsmuller, Willy M Baarends   +8 more
doaj   +1 more source

Transcriptional profiling of colicin-induced cell death of Escherichia coli MG1655 identifies potential mechanisms by which bacteriocins promote bacterial diversity [PDF]

, 2004
We report the transcriptional response of Escherichia coli MG1655 to damage induced by colicins E3 and E9, bacteriocins that kill cells through inactivation of the ribosome and degradation of chromosomal DNA, respectively. Colicin E9 strongly induced the
Hinton, Jay C. D., James, Richard, Kleanthous, Colin, Moore, Geoffrey R., Rolfe, Matthew, Thompson, Arthur, WALKER, DANIEL   +6 more
core   +2 more sources

The carboxy-terminal αN helix of the archaeal XerA tyrosine recombinase is a molecular switch to control site-specific recombination.

PLoS ONE, 2013
Tyrosine recombinases are conserved in the three kingdoms of life. Here we present the first crystal structure of a full-length archaeal tyrosine recombinase, XerA from Pyrococcus abyssi, at 3.0 Å resolution.
Marie-Claude Serre, Toufic El Arnaout, Mark A Brooks, Dominique Durand, Johnny Lisboa, Noureddine Lazar, Bertrand Raynal, Herman van Tilbeurgh, Sophie Quevillon-Cheruel   +8 more
doaj   +1 more source

Mechanistic modelling of a recombinase-based two-input temporal logic gate [PDF]

, 2017
Site-specific recombinases (SSRs) mediate efficient manipulation of DNA sequences in vitro and in vivo. In particular, serine integrases have been identified as highly effective tools for facilitating DNA inversion, enabling the design of genetic ...
Bates, Declan, Bowyer, Jack E., Hsiao, Victoria, Wong, Wilson W.   +3 more
core   +1 more source

Alignment of Homologous Chromosomes and Effective Repair of Programmed DNA Double-Strand Breaks during Mouse Meiosis Require the Minichromosome Maintenance Domain Containing 2 (MCMDC2) Protein. [PDF]

PLoS Genetics, 2016
Orderly chromosome segregation during the first meiotic division requires meiotic recombination to form crossovers between homologous chromosomes (homologues).
Friederike Finsterbusch, Ramya Ravindranathan, Ihsan Dereli, Marcello Stanzione, Daniel Tränkner, Attila Tóth   +5 more
doaj   +1 more source

Mutants of Cre recombinase with improved accuracy [PDF]

, 2014
Despite rapid advances in genome engineering technologies, inserting genes into precise locations in the human genome remains an outstanding problem. It has been suggested that site-specific recombinases can be adapted towards use as transgene delivery ...
Church, George M., Eroshenko, Nikolai
core   +1 more source

Serine integrase chimeras with activity in E. coli and HeLa cells

Biology Open, 2014
In recent years, application of serine integrases for genomic engineering has increased in popularity. The factor-independence and unidirectionality of these large serine recombinases makes them well suited for reactions such as site-directed vector ...
Alfonso P. Farruggio, Michele P. Calos
doaj   +1 more source

Control of serine integrase recombination directionality by fusion with the directionality factor [PDF]

, 2017
Bacteriophage serine integrases are extensively used in biotechnology and synthetic biology for assembly and rearrangement of DNA sequences. Serine integrases promote recombination between two different DNA sites, attP and attB, to form recombinant attL ...
Akopian, Arlene L. McPherson, Arnold, Bonnet, Bonnet, Brophy, Brown, Colloms, Fan, Farruggio, Femi J. Olorunniji, Fogg, Ghosh, Khaleel, Kilbride, Kuhstoss, Ladurner, Lewis, Mandali, Maranhao, Margaret C.M. Smith, Moore, Olorunniji, Olorunniji, Olorunniji, Olorunniji, Olorunniji, Pokhilko, Prorocic, Proudfoot, Roquet, Rowley, Rutherford, Rutherford, Sean D. Colloms, Smith, Susan J. Rosser, Van Duyne, W. Marshall Stark, Weinberg, Xu, Yang, Zhang, Zhang   +43 more
core   +3 more sources