Results 251 to 260 of about 4,058,802 (278)

Structure and organization of the human reduced folate carrier gene

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1998
The human reduced folate carrier gene was found to contain 7 exons, including two alternative non-coding exons (exons 1 and 2), spanning approximately 29 kb. Two transcript variants involving exon 7 were detected in K562 cells by RT-PCR, distinguishable from the wild-type transcript by deletions of 625 bp (KS32) and 988 bp (KS1).
L, Zhang, S C, Wong, L H, Matherly
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Comparison of Transport Properties of the Reduced Folate Carrier and Folate Receptor in Murine L1210 Leukemia Cells

Biochemical Pharmacology, 1995
This laboratory previously described an L1210 murine leukemia cell line with a functional defect in the reduced folate carrier and increased expression of folate receptor-beta (F2-MTXrA). This cell line was used to characterize methotrexate (MTX) influx mediated by folate receptor-beta and to compare this with influx mediated by the reduced folate ...
Michael J Spinella, Kevin E Brigle, Esteban E Sierra   +2 more
exaly   +3 more sources

Anion specificity of the jejunal folate carrier: Effects of reduced folate analogues on folate uptake and efflux

The Journal of Membrane Biology, 1988
We previously reported that 3H-folate uptake by rabbit jejunal brush-border membrane (BBM) vesicles was markedly stimulated by an outwardly directed OH- gradient (pHin 7.7, pHout 5.5), inhibited by anion exchange inhibitors (DIDS, SITS, furosemide), and saturable (folate Km = 0.19 microM) suggesting carrier-mediated folate/OH- exchange (or H+/folate ...
C M, Schron, C, Washington, B L, Blitzer
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G80A Reduced Folate Carrier SNP Influences the Absorption and Cellular Translocation of Dietary Folate and Its Association With Blood Pressure in an Elderly Population

Life Sciences, 2006
The functional consequences of the G80A RFC SNP on the expressed reduced folate carrier protein were evaluated by looking at the relationship between intake of folate, plasma folate and cellular stores of the vitamin.
Mark Lucock, Nenad Naumovski, Peter Lewis   +2 more
exaly   +2 more sources

A Polymorphism (80G->A) in the Reduced Folate Carrier Gene and Its Associations with Folate Status and Homocysteinemia

Molecular Genetics and Metabolism, 2000
5-methyltetrahydrofolate is the predominant form of folate in plasma. It may be the preferred substrate for transport via the reduced-folate carrier (RFC). We isolated a cDNA for the reduced folate carrier (RFC-1) from human skin fibroblasts. A common polymorphism at position 80 in exon 2 of RFC-1 was identified. This polymorphism changes a guanine (G)
A, Chango, N, Emery-Fillon, G P, de Courcy, D, Lambert, M, Pfister, D S, Rosenblatt, J P, Nicolas   +6 more
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Kinetics of Reduced Folate Carrier-and Membrane-Associated Folate Receptor-Mediated Transport of Antifolates

2002
Several distinct routes for the uptake of folates and antifolates have been reported. Transport via the reduced folate carrier (RFC) is a carrier-mediated process that generates folate uptake through a bi-directional anion exchange mechanism (1,2). The RFC is characterized by a high affinity (Km: 1–10 µM) for reduced folates and MTX, whereas it has a ...
Mauritz, R., Peters, GJ, Kathmann, G. A. M., Rosowsky, Andre, Pinedo, HM, Jansen, G   +5 more
openaire   +3 more sources

Molecular and cellular biology of the human reduced folate carrier

2001
The natural folates are water-soluble members of the B class of vitamins that are essential for cell proliferation and tissue regeneration. Since mammalian cells cannot synthesize folates de novo, tightly regulated and sophisticated cellular uptake processes have evolved to sustain sufficient levels of intracellular tetrahydrofolate cofactors to ...
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Interactions of human reduced folate carrier SLC19A1 with folates and antifolates

Biophysical Journal, 2023
Han Zhang, Wonpil Im
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Abstract 873: Acute regulation of human reduced folate carrier by folates as a novel adaptive mechanism to folate deprivation.

Cancer Research, 2013
Abstract The reduced folate carrier (RFC) is the major tissue folate transporter in mammalian cells. RFC is essential for providing folates for biosynthetic pathways leading to nucleotides and amino acids. Clinically relevant antifolates including methotrexate (Mtx), pemetrexed, and pralatrexate are all substrates for RFC and RFC levels ...
Zhanjun Hou, Steve Orr, Erika Etnyre, Christina Cherian, Larry Matherly   +4 more
openaire   +1 more source

Loss of Reduced Folate Carrier Function and Folate Depletion Result in Enhanced Pemetrexed Inhibition of Purine Synthesis

Clinical Cancer Research, 2005
Abstract Pemetrexed is a novel antifolate with polyglutamate derivatives that are potent inhibitors of thymidylate synthase (TS) and to a lesser extent glycinamide ribonucleotide formyltransferase (GARFT). Conditions that might modulate relative suppression of these sites were assessed by the pattern of hypoxanthine and thymidine ...
Rongbao, Zhao, Shubing, Zhang, Marie, Hanscom, Shrikanta, Chattopadhyay, I David, Goldman   +4 more
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