Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics
FEBS Letters, EarlyView.Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt +8 more
wiley +1 more source
Identification and Validation of Reference Genes for Quantitative Real-Time PCR in Ficus carica
PlantsFig (Ficus carica L.), a deciduous fruit tree that belongs to the Moraceae family, is cultivated worldwide as an important fruit crop for raw and processed foods. Quantitative real-time PCR (RT-qPCR) is a widely used method in F.
Masahito Nakano
doaj +1 more source
Gene expression studies: How to obtain accurate and reliable data by quantitative real-time RT PCR [PDF]
Journal of Medical Biochemistry, 2013 Real-time RT PCR has been recognized as an accurate, reliable and sensitive method for quantifying gene transcription. However, several steps preceding PCR represent critical points and source of inaccuracies.
Nestorov Jelena +3 more
doaj
Outstanding Business Reference Sources: The 2009 Selection of Recent Titles
, 2010 A selection of business reference sources for ...
BRASS Business Reference Sources Committee
core
Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation
FEBS Letters, EarlyView.Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang +2 more
wiley +1 more source
Screening and validation of reference genes for quantitative real-time PCR in Polygonatum hunanense
Medicinal Plant BiologyPolygonatum hunanense H.H. Liu & B.Z. Wang (syn. P. kingianum var. grandifolium), is a perennial Liliaceae species with ethnopharmaceutical and nutraceutical value. It exhibits traditional medicinal properties such as qi-tonifying, yin-nourishing, kidney-
Chensi Tan +7 more
doaj +1 more source
Outstanding Business Reference Sources: The 2003 Selection of Recent Titles
, 2004 A selection of business reference sources for ...
BRASS Business Reference Sources Committee
core
Differences in Gene Expression between Sonoporation in Tumor and in Muscle
, 2010 Background Ultrasound (US) is a novel and effective tool for the local delivery of genes into target tissues. US can temporarily change the permeability of a cell membrane and thus enhance the delivery of naked DNA into cells.
TSAI, KUN-CHE;LIAO, ZHE-KANG;LIN, WIN-LI;SHIEH, MING-JIUM;HWAN,G LIH-HWA;CHEN, WEN-SHIANG +1 more
core +1 more source
Liver organoids: modelling complexity in homeostasis and disease
FEBS Letters, EarlyView.Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley +1 more source
Screening and Identification of Reference Genes for Paracarophenax alternatus
InsectsIn this study, we focused on the screening and identification of reference genes for Paracarophenax alternatus Xu and Zhang. The laboratory population was used as the laboratory population, and samples were collected from mites at four different stages, including physogastry, viviparous, 5 d viviparous and phoresy.
Yangming Zhang +6 more
openaire +2 more sources