Results 1 to 10 of about 1,765,900 (302)

Custom selected reference genes outperform pre-defined reference genes in transcriptomic analysis [PDF]

BMC Genomics, 2020
Background RNA sequencing allows the measuring of gene expression at a resolution unmet by expression arrays or RT-qPCR. It is however necessary to normalize sequencing data by library size, transcript size and composition, among other factors, before ...
Karen Cristine Gonçalves dos Santos, Isabel Desgagné-Penix, Hugo Germain   +2 more
doaj   +4 more sources

Effective Alu repeat based RT-qPCR normalization in cancer cell perturbation experiments [PDF]

PLoS ONE, 2013
Background: Measuring messenger RNA (mRNA) levels using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) is common practice in many laboratories.
Beckers, Anneleen, De Brouwer, Sara, Kumps, Candy, Leonelli, Carina, Mestdagh, Pieter, Mets, Evelien, Pattyn, Filip, Rihani, Ali, Rondou, Pieter, Speleman, Franki, Van der Meulen, Joni, Van Maerken, Tom, Van Peer, Gert, Vandesompele, Jo   +13 more
core   +20 more sources

Screening and Identification of Reference Genes for Paracarophenax alternatus [PDF]

Insects
In this study, we focused on the screening and identification of reference genes for Paracarophenax alternatus Xu and Zhang. The laboratory population was used as the laboratory population, and samples were collected from mites at four different stages ...
Yangming Zhang, Xu Chu, Ruiheng Lin, Yunfeng Zheng, Sikai Ke, Feiping Zhang, Songqing Wu   +6 more
doaj   +2 more sources

Reference Genes for Expression Analyses by qRT-PCR in Propsilocerus akamusi (Diptera: Chironomidae) [PDF]

Biology
Propsilocerus akamusi (Tokunaga, 1938) larvae serve as key bioindicators for water quality assessment. This study identifies optimal reference genes for RT-qPCR under diverse experimental conditions.
Wenbin Liu, Yaning Tang, Ziming Shao, Jiaxin Nie, Xue Bai, Zhe Nie, Chunmian Liu, Yajin Zhang, Chuncai Yan, Yiwen Wang   +9 more
doaj   +2 more sources

Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in Megalurothrips usitatus (thysanoptera: thripidae)

Frontiers in Physiology, 2023
IntroductionGene expression analysis by reverse transcription quantitative polymerase chain reaction (qRT-PCR) has been widely used in research including insects.
Qingfang Hou, Qingfang Hou, Linlin Yuan, Linlin Yuan, Haifeng Jin, Haifeng Jin, Han Yan, Han Yan, Fen Li, Fen Li, Shaoying Wu, Shaoying Wu   +11 more
doaj   +1 more source

Transcriptome-wide selection and validation of a solid set of reference genes for gene expression studies in the cephalopod mollusk Octopus vulgaris

Frontiers in Molecular Neuroscience, 2023
Octopus vulgaris is a cephalopod mollusk and an active marine predator that has been at the center of a number of studies focused on the understanding of neural and biological plasticity.
Pamela Imperadore, Stefano Cagnin, Stefano Cagnin, Vittoria Allegretti, Caterina Millino, Francesca Raffini, Graziano Fiorito, Giovanna Ponte   +7 more
doaj   +1 more source

Selection of reference genes suitable for normalization of RT-qPCR data in glioma stem cells

BioTechniques, 2020
Considering the importance of gene expression studies for understanding the biology of glioma stem cells (GSCs), we aimed to identify the reliable reference genes in GSCs that were derived from the glioma cell lines T98G, LN229, 090116 and 091214 ...
Weiqi Dang, Xiang Zhang, Qinghua Ma, Lu Chen, Mianfu Cao, Jingya Miao, Youhong Cui, Xia Zhang   +7 more
doaj   +1 more source

Evaluation and validation of reference genes in Cymbidium faberi for real-time quantitative PCR

BioTechniques, 2022
For all organs at all Cymbidium faberi stages, ACT, UBQ3 and GAPDH can be selected as reference genes. For organs of the vegetative stage, UBQ2 and UBQ3 can be chosen for analysis of normalized gene expression. For the bud stage, ACT and UBQ3 can be used
Yunfang Tian, Zhigang Chu, Linqing Wang, Huiyu Wang, Xiuyun Yuan, Si Wu, Yuzhen Yang   +6 more
doaj   +1 more source

Systematic Identification of Suitable Reference Genes for Quantitative Real-Time PCR Analysis in Melissa officinalis L

Plants, 2023
Melissa officinalis L. is well known for its lemon-scented aroma and various pharmacological properties. Despite these valuable properties, the genes involved in the biosynthetic pathways in M. officinalis are not yet well-explored when compared to other
Rohit Bharati, Madhab Kumar Sen, Ram Kumar, Aayushi Gupta, Jana Žiarovská, Eloy Fernández-Cusimamani, Olga Leuner   +6 more
doaj   +1 more source

Selection and Evaluation of Reference Genes for Quantitative Real-Time PCR in Tomato (Solanum lycopersicum L.) Inoculated with Oidium neolycopersici

Agronomy, 2022
In order to screen out the most stable reference genes in tomatoes under powdery mildew (PM) stress and study the expression of related genes in the interaction between tomato and PM more accurately, this study will provide a calibration basis for the ...
Shengyi Bai, Xiaomin Wang, Meng Guo, Guoxin Cheng, Abid Khan, Wenkong Yao, Yanming Gao, Jianshe Li   +7 more
doaj   +1 more source