Results 11 to 20 of about 1,765,900 (302)

Correction to: Custom selected reference genes outperform pre-defined reference genes in transcriptomic analysis [PDF]

open access: yesBMC Genomics, 2021
Karen Cristine Gonçalves dos Santos   +2 more
doaj   +3 more sources

Reference genes in real-time PCR [PDF]

open access: yesJournal of Applied Genetics, 2013
This paper aims to discuss various aspects of the use of reference genes in qPCR technique used in the thousands of present studies. Most frequently, these are housekeeping genes and they must meet several criteria so that they can lay claim to the name.
Kozera, Bartłomiej, Rapacz, Marcin
openaire   +2 more sources

RefPrimeCouch—a reference gene primer CouchApp [PDF]

open access: yesDatabase, 2013
To support a quantitative real-time polymerase chain reaction standardization project, a new reference gene database application was required. The new database application was built with the explicit goal of simplifying not only the development process but also making the user interface more responsive and intuitive.
Silbermann, Jascha   +3 more
openaire   +3 more sources

Reference genes for measuring mRNA expression [PDF]

open access: yesTheory in Biosciences, 2012
The aim of this review is to find answers to some of the questions surrounding reference genes and their reliability for quantitative experiments. Reference genes are assumed to be at a constant expression level, over a range of conditions such as temperature.
Dundas, J, Ling, M
openaire   +3 more sources

Identification of stable senescence‐associated reference genes [PDF]

open access: yesAging Cell, 2019
AbstractCellular senescence is a state of permanent cell cycle arrest activated in response to damaging stimuli. Many hallmarks associated with senescent cells are measured by quantitative real‐time PCR (qPCR). As the selection of stable reference genes for interpretation of qPCR data is often overlooked, we performed a systematic review to understand ...
Alejandra Hernandez‐Segura   +2 more
openaire   +2 more sources

Reference-based QUantification Of gene Dispensability (QUOD) [PDF]

open access: yesPlant Methods, 2020
Abstract Background Dispensability of genes in a phylogenetic lineage, e.g. a species, genus, or higher-level clade, is gaining relevance as most genome sequencing projects move to a pangenome level.
Frey, Katharina   +2 more
openaire   +5 more sources

Reference genes for gene expression studies in the mouse heart [PDF]

open access: yesScientific Reports, 2017
AbstractTo be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable reference genes for normalization. This is especially critical in cardiac research because of the diversity of the clinical and experimental conditions in the field.
Ruiz-Villalba, Adrián   +5 more
openaire   +6 more sources

Effect of Nitrogen Sources on Omega-3 Polyunsaturated Fatty Acid Biosynthesis and Gene Expression in Thraustochytriidae sp.

open access: yesMarine Drugs, 2020
The molecular mechanism that contributes to nitrogen source dependent omega-3 polyunsaturated fatty acid (n-3 PUFA) synthesis in marine oleaginous protists Thraustochytriidae sp., was explored in this study.
Siting Li   +3 more
doaj   +1 more source

Identification of new reference genes for colony counting by reverse-transcription quantitative PCR in Bifidobacterium animalis

open access: yesJournal of Dairy Science, 2023
: Bifidobacterium animalis, one of the predominant bacteria in the intestines of humans and other mammals, is widely added to dairy products. We employed RNA sequencing to analyze gene expression variance on a genome-wide scale and found stable reference
Yaping Liu   +6 more
doaj   +1 more source

Drought and Oxidative Stress in Flax (Linum usitatissimum L.) Entails Harnessing Non-Canonical Reference Gene for Precise Quantification of qRT-PCR Gene Expression

open access: yesAntioxidants, 2023
Flax (Linum usitatissimum L.) is a self-pollinating, annual, diploid crop grown for multi-utility purposes for its quality oil, shining bast fiber, and industrial solvent.
Prasanta K. Dash   +6 more
doaj   +1 more source

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