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Identification of endogenous reference genes for RT-qPCR analysis in breast cancer and matched adjacent tissues [PDF]
Frontiers in OncologyBackgroundReal-time quantitative PCR (RT-qPCR), essential for gene expression and biomarker studies, requires stable endogenous reference genes (RGs) for normalization. This study aimed to identify consistently expressed RGs in breast cancer and adjacent
Yue Meng +8 more
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Frontiers in Microbiology, 2021 Alicyclobacillus acidoterrestris is a major concern in fruit juice industry due to its spoilage potential of acidic fruit juice. Quantifying the expression levels of functional genes by real-time quantitative polymerase chain reaction (RT-qPCR) is ...
Mingtao Fan +2 more
exaly +3 more sources
Selection and validation of suitable reference genes for qPCR expression analysis in young in vitro grown kohlrabi under cytokinin and sucrose treatments [PDF]
Frontiers in Plant ScienceReference genes (RGs) commonly serve as internal controls for normalization of gene expression data in quantitative real-time polymerase chain reaction (qPCR) analyses.
Anđela Tomić +6 more
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Frontiers in Physiology, 2021 Rhopalosiphum padi (L.) (Hemiptera: Aphididae) is an important cosmopolitan pest in cereal crops. Reference genes can significantly affect qRT-PCR results. Therefore, selecting appropriate reference genes is a key prerequisite for qRT-PCR analyses.
Xun Zhu, Fengshan Yang
exaly +3 more sources
Evaluation and Selection of Stable Reference Genes for qRT-PCR Analysis in Different Tissues of Mugilogobius chulae Under Pollutant Exposure [PDF]
AnimalsReliable normalization is essential for accurate quantitative real-time PCR (qRT-PCR) analysis, yet suitable reference genes have not been systematically evaluated in Mugilogobius chulae, an emerging marine experimental fish used in physiological and ...
Zhongdian Dong +9 more
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Selection of appropriate reference genes in Apoe-/- mouse brain [PDF]
IBRO Neuroscience ReportsBackground: Reverse transcription quantitative real-time polymerase chain reaction is the gold standard for gene expression quantification. Yet, this method’s accuracy heavily depends on choosing appropriate reference genes for data normalization ...
Naz Mengi Camur +4 more
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Reference Genes for Expression Analyses by RT-qPCR in Hyblaea puera (Lepidoptera: Hyblaeidae) [PDF]
InsectsHyblaea puera is a major pest of teak and mangroves. Reliable RT-qPCR normalization requires stable reference genes, none of which have been validated in H. puera. In this study, we assessed the expression stability of ten candidate reference genes under
Xinan Li +9 more
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Frontiers in Plant Science, 2022 Gene expression analysis largely improves our understanding of the molecular basis underpinning various plant biological processes. Stable reference genes play a foundational role during the normalization of gene expression levels.
Junliang Yin, Qie Jia, Peihua Yang
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Frontiers in PhysiologyMylabris sibirica is a hypermetamorphic insect whose adults feed on oilseed rape. However, due to a shortage of effective and appropriate endogenous references, studies on molecular functional genes in Mylabris sibirica, have been tremendously limited ...
Chenhui Shen
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Identification of a novel real-time PCR reference gene panel for EA.hy926 endothelial cells in different growth States and with DHA treatment [PDF]
Scientific ReportsEndothelial dysfunction underlies the pathogenesis of many diseases, including atherosclerosis, diabetes, and kidney failure. The EA.hy926 cell line is one of the most widely used human macrovascular endothelial cell models, and has been shown to enter ...
Shiqi Huang +5 more
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