Results 11 to 20 of about 2,631 (175)

Selection and Validation of Reference Genes for RT-qPCR Analysis of the Ladybird Beetle Henosepilachna vigintioctomaculata

Frontiers in Physiology, 2018
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a momentous technique for quantifying expression levels of the targeted genes across various biological processes.
Bao-Li Qiu, Huipeng Pan, Shimin Chen
exaly   +3 more sources

Gene Expression Profile Analysis is Directly Affected by the Selected Reference Gene: The Case of Leaf-Cutting Atta Sexdens

Insects, 2018
Although several ant species are important targets for the development of molecular control strategies, only a few studies focus on identifying and validating reference genes for quantitative reverse transcription polymerase chain reaction (RT-qPCR) data
Ronald Zanetti, Luciano Paiva, Kalynka Livramento   +2 more
exaly   +3 more sources

Evaluation of Sorghum [Sorghum bicolor (L.)] Reference Genes in Various Tissues and under Abiotic Stress Conditions for Quantitative Real-Time PCR Data Normalization

Frontiers in Plant Science, 2016
Accurate and reliable gene expression data from qPCR depends on stable reference gene expression for potential gene functional analyses. In this study, 15 reference genes were selected and analyzed in various sample sets including abiotic stress ...
Pooja Bhatnagar-Mathur, Sudhakar Reddy Palakolanu, Vincent Vadez   +2 more
exaly   +3 more sources

Characterization of reference genes for RT-qPCR in the desert moss Syntrichia caninervis in response to abiotic stress and desiccation/rehydration

Frontiers in Plant Science, 2015
Syntrichia caninervis is the dominant bryophyte of the biological soil crusts found in the Gurbantunggut desert. The extreme desert environment is characterized by prolonged drought, temperature extremes, high radiation and frequent cycles of hydration ...
Zhang Daoyuan, Andrew Wood, Bei Gao
exaly   +3 more sources

Expression Stability Analysis of Candidate References for Normalization of RT-qPCR Data Using RefSeeker R package

Bio-Protocol, 2023
When performing expression analysis either for coding RNA (e.g., mRNA) or non-coding RNA (e.g., miRNA), reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a widely used method.
Patrick Petersen, Joanna Lopacinska-Jørgensen, Claus Høgdall, Estrid Høgdall   +3 more
doaj   +1 more source

Selection and Validation of Potential Reference Genes for Quantitative Real-Time PCR Analysis in Blaptica Dubia (Serville, 1838) (Blattidae, Blaberidae)

Cumhuriyet Science Journal, 2022
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is an effective, reproducible, and dependable method for evaluating and targeting expression of genes.
Emin Ufuk Karakaş, Şeyda Berk, Ayşe Nur Pektaş   +2 more
doaj   +1 more source

Reference gene validation for RT-qPCR, a note on different available software packages. [PDF]

PLoS ONE, 2015
An appropriate normalization strategy is crucial for data analysis from real time reverse transcription polymerase chain reactions (RT-qPCR). It is widely supported to identify and validate stable reference genes, since no single biological gene is ...
Ward De Spiegelaere, Jutta Dern-Wieloch, Roswitha Weigel, Valérie Schumacher, Hubert Schorle, Daniel Nettersheim, Martin Bergmann, Ralph Brehm, Sabine Kliesch, Linos Vandekerckhove, Cornelia Fink   +10 more
doaj   +1 more source

Stable Reference Gene Selection for RT-qPCR Analysis in Nonviruliferous and Viruliferous Frankliniella occidentalis. [PDF]

PLoS ONE, 2015
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for measuring and evaluating gene expression during variable biological processes.
Chunxiao Yang, Hui Li, Huipeng Pan, Yabin Ma, Deyong Zhang, Yong Liu, Zhanhong Zhang, Changying Zheng, Dong Chu   +8 more
doaj   +1 more source

Identification of stable housekeeping genes for induced pluripotent stem cells and -derived endothelial cells for drug testing

Scientific Reports, 2022
There are no validated housekeeping genes in induced pluripotent stem cells (iPSC) and derived endothelial iPSC (iPSC-EC). Thus a comparison of gene expression levels is less reliable, especially during drug treatments.
Sheena L. M. Ong, Hans J. Baelde, David G. P. van IJzendoorn, Judith V. M. G. Bovée, Karoly Szuhai   +4 more
doaj   +1 more source

Selection of housekeeping genes and demonstration of RNAi in cotton leafhopper, Amrasca biguttula biguttula (Ishida). [PDF]

PLoS ONE, 2018
Amrasca biguttula biguttula (Ishida) commonly known as cotton leafhopper is a severe pest of cotton and okra. Not much is known on this insect at molecular level due to lack of genomic and transcriptomic data. To prepare for functional genomic studies in
Satnam Singh, Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Pankaj Rathore, Subba Reddy Palli   +5 more
doaj   +1 more source