Results 21 to 30 of about 2,631 (175)

Evaluation of Reference Genes for Quantitative Reverse Transcription Polymerase Chain Reaction in Bactrocera dorsalis (Diptera: Tephritidae) Subjected to Various Phytosanitary Treatments

Insects, 2021
Bactrocera dorsalis is a major pest that causes serious damage to many fruits. Although phytosanitary treatment methods have been developed for Bactrocera control, there is a lack of information related to the gene expression pattern of B.
Yue Cao, Baishu Li, Naizhong Chen, Ding Yang, Li Li, Tao Liu   +5 more
doaj   +1 more source

Selection and Evaluation of Candidate Reference Genes for Quantitative Real-Time PCR in Aboveground Tissues and Drought Conditions in Rhododendron Delavayi

Frontiers in Genetics, 2022
There has been no systematic identification and screening of candidate reference genes for normalization of quantitative real-time PCR (qRT-PCR) results in Rhododendron delavayi to date.
Lu Zhang, Lu Zhang, Yanfei Cai, Yanfei Cai, Mingchao Zhang, Guanghui Du, Jihua Wang, Jihua Wang   +7 more
doaj   +1 more source

Eleven candidate reference genes ranked under various conditions using RefFinder.

, 2022
Eleven candidate reference genes ranked under various conditions using RefFinder.
Hao Zhan (6853280), Lin Liu (74495), Tianchong Wang (12475355), Xiujun Lu (8482328), Wanfeng Ai (12475358), Hanzhang Liu (12183474)   +5 more
core   +1 more source

IDENTIFICATION AND VALIDATION OF REFERENCE GENES FOR THE NORMALIZATION IN REAL-TIME RT-QPCR ON RICE AND RED RICE IN COMPETITION, UNDER DIFFERENT NITROGEN DOSES [PDF]

Planta Daninha, 2017
Real time reverse transcription polymerase chain reaction (RT-qPCR) is an important technique to analyze differences in gene expression due to its sensitivity, accuracy, and specificity.
D.P. BENEMANN, A.M. NOHATO, L. VARGAS, L.A AVILA, D. AGOSTINETTO   +4 more
doaj   +1 more source

Identification and validation of stable reference genes for quantitative real time PCR in different minipig tissues at developmental stages

BMC Genomics, 2022
Background Quantitative real time PCR (qPCR) is a powerful tool to evaluate mRNA expression level. However, reliable qPCR results require normalization with validated reference gene(s).
Jeongah Song, Jeonghee Cho, Jeongsik Park, Jeong Ho Hwang   +3 more
doaj   +1 more source

Identification of Reliable Reference Genes for the Expression of Hydrangea macrophylla ‘Bailmer’ and ‘Duro’ Sepal Color

Horticulturae, 2022
Hydrangea spp. is renowned for its variety of color changes in its developmental stage and before and after aluminum treatment. We analyzed gene expression in hydrangeas sepals to study the causes of color change.
Gaitian Zhang, Suxia Yuan, Hui Qi, Zhiyun Chu, Chun Liu   +4 more
doaj   +1 more source

Selection and Evaluation of Reference Genes for Quantitative Real-Time PCR in Tomato (Solanum lycopersicum L.) Inoculated with Oidium neolycopersici

Agronomy, 2022
In order to screen out the most stable reference genes in tomatoes under powdery mildew (PM) stress and study the expression of related genes in the interaction between tomato and PM more accurately, this study will provide a calibration basis for the ...
Shengyi Bai, Xiaomin Wang, Meng Guo, Guoxin Cheng, Abid Khan, Wenkong Yao, Yanming Gao, Jianshe Li   +7 more
doaj   +1 more source

Reference genes for normalization of qPCR assays in sugarcane plants under water deficit

Plant Methods, 2017
Background Sugarcane (Saccharum spp.) is the main raw material for sugar and ethanol production. Among the abiotic stress, drought is the main one that negatively impact sugarcane yield.
Larissa Mara de Andrade, Michael dos Santos Brito, Rafael Fávero Peixoto Junior, Paulo Eduardo Ribeiro Marchiori, Paula Macedo Nóbile, Alexandre Palma Boer Martins, Rafael Vasconcelos Ribeiro, Silvana Creste   +7 more
doaj   +1 more source

Evaluation of Reference Genes for Quantitative PCR in Four Tissues from Rabbits with Hypercholesterolaemia [PDF]

Brazilian Archives of Biology and Technology, 2019
Rabbit with hypercholesterolaemia is an important model for studying cholesterol metabolism disease. This study aimed to evaluate the expression stability of nine reference genes for quantitative PCR (qPCR) analysis in adrenal gland, liver, spleen, and ...
Zhen Zhang, Bin Wen, Yuan Xu, En-ze Jiang, Jia-yu Liu, Ke-li Zhu, Fang-yong Ning, Zhi-Heng Du, Xiu-Juan Bai   +8 more
doaj   +1 more source

Putative Internal Control Genes in Bovine Milk Small Extracellular Vesicles Suitable for Normalization in Quantitative Real Time-Polymerase Chain Reaction

Membranes, 2021
Bovine milk small extracellular vesicles (sEVs) contain many biologically important molecules, including mRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) is a widely used method for quantifying mRNA in tissues and cells. However, the use,
Md. Matiur Rahman, Shigeo Takashima, Yuji O. Kamatari, Yassien Badr, Kaori Shimizu, Ayaka Okada, Yasuo Inoshima   +6 more
doaj   +1 more source