Rapid nuclear deadenylation of mammalian messenger RNA
iScience, 2023 Summary: Poly(A) tails protect RNAs from degradation and their deadenylation rates determine RNA stability. Although poly(A) tails are generated in the nucleus, deadenylation of tails has mostly been investigated within the cytoplasm.
Jonathan Alles +3 more
doaj +1 more source
Regulatory elements of the erythropoietin gene [PDF]
Blood, 1991 Abstract Because the human hepatoma cell line Hep3B produces erythropoietin (Epo) in a regulated fashion, it can be used to investigate the cis- acting regulatory elements of the Epo gene. Comparison of primate and mouse sequences shows strong homology not only in the coding sequence but also within the 5′ flanking region, the first ...
S, Imagawa +3 more
openaire +3 more sources
Conservation of mRNA and Protein Expression during Development of C. elegans
Cell Reports, 2014 Spatiotemporal control of gene expression is crucial for development and subject to evolutionary changes. Although proteins are the final product of most genes, the developmental proteome of an animal has not yet been comprehensively defined, and the ...
Dominic Grün +5 more
doaj +1 more source
Ancient Pbx-Hox signatures define hundreds of vertebrate developmental enhancers [PDF]
, 2011 Background: Gene regulation through cis-regulatory elements plays a crucial role in development and disease. A major aim of the post-genomic era is to be able to read the function of cis-regulatory elements through scrutiny of their DNA sequence ...
Bronner, Marianne E. +4 more
core +5 more sources
cis-regulatory circuits regulating NEK6 kinase overexpression in transformed B cells Are super-enhancer independent [PDF]
, 2017 Alterations in distal regulatory elements that control gene expression underlie many diseases, including cancer. Epigenomic analyses of normal and diseased cells have produced correlative predictions for connections between dysregulated enhancers and ...
Huang, Yue +6 more
core +3 more sources
Cell fixation and preservation for droplet-based single-cell transcriptomics
BMC Biology, 2017 Background Recent developments in droplet-based microfluidics allow the transcriptional profiling of thousands of individual cells in a quantitative, highly parallel and cost-effective way.
Jonathan Alles +12 more
doaj +1 more source
Mapping open chromatin by ATAC-seq in bread wheat
Frontiers in Plant Science, 2022 Gene transcription is largely regulated by cis-regulatory elements. Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) is an emerging technology that can accurately map cis-regulatory elements in animals and plants.
Xin Wang +4 more
doaj +1 more source
Systematic discovery of structural elements governing stability of mammalian messenger RNAs. [PDF]
, 2012 Decoding post-transcriptional regulatory programs in RNA is a critical step towards the larger goal of developing predictive dynamical models of cellular behaviour.
Cristea, Ileana M +7 more
core +2 more sources
Post-transcriptional Regulation by 3′ UTRs Can Be Masked by Regulatory Elements in 5′ UTRs
Cell Reports, 2018 Summary: In mRNA sequences, 3′ UTRs are thought to contain most elements that specifically regulate localization, turnover, and translation. Although high-throughput experiments indicate that many RNA-binding proteins (RBPs) also bind 5′ UTRs, much less ...
Kathrin Theil +2 more
doaj +1 more source
Tracing tumorigenesis in a solid tumor model at single-cell resolution
Nature Communications, 2020 Understanding tumour development at a granular level is a challenge in solid tumours. Here, the authors provide a cell atlas across tumour development in a genetic model of salivary gland squamous cell carcinoma using single-cell transcriptome and ...
Samantha D. Praktiknjo +9 more
doaj +1 more source