Results 61 to 70 of about 264,833 (258)
Using electrostatic potentials to predict DNA-binding sites on DNA-binding proteins [PDF]
, 2003 A method to detect DNA-binding sites on the surface of a protein structure is important for functional annotation. This work describes the analysis of residue patches on the surface of DNA-binding proteins and the development of a method of predicting ...
Berman, Helen M +3 more
core +4 more sources
Cleavage of the lambda and P22 repressors by recA protein.
Journal of Biological Chemistry, 1982 The site of recA cleavage of the phage lambda and P22 repressors has been determined. Each repressor is cut once by the recA enzyme, lambda repressor between residues 111 and 112, and P22 repressor between residues 94 and 95. recA cleavage occurs at identical alanyl-glycyl sequences in both repressors, and in both repressors, the cleavage separates the
Robert T. Sauer, Mark Ptashne, M J Ross
openaire +3 more sources
Transcription Past Loop Forming Repressor Proteins [PDF]
Biophysical Journal, 2017 Topology plays a major role in the transcriptional regulation of genetic information. Among the most ubiquitous topological elements are loops that form when proteins simultaneously bind to two non-contiguous sites along the DNA. This topology increases the local concentration of the protein around each of the sites and effectively stabilizes the bound
Zsuzsanna Vörös +2 more
openaire +2 more sources
Stochastic variation in the FOXM1 transcription program mediates replication stress tolerance
Molecular Oncology, EarlyView.Cellular heterogeneity is a major cause of drug resistance in cancer. Segeren et al. used single‐cell transcriptomics to investigate gene expression events that correlate with sensitivity to the DNA‐damaging drugs gemcitabine and prexasertib. They show that dampened expression of transcription factor FOXM1 and its target genes protected cells against ...
Hendrika A. Segeren +4 more
wiley +1 more source
What makes the lac-pathway switch: identifying the fluctuations that trigger phenotype switching in gene regulatory systems [PDF]
, 2014 Multistable gene regulatory systems sustain different levels of gene expression under identical external conditions. Such multistability is used to encode phenotypic states in processes including nutrient uptake and persistence in bacteria, fate ...
Berg, Johannes +3 more
core +2 more sources
LexA protein is a repressor of the colicin E1 gene.
Journal of Biological Chemistry, 1983 LexA protein is a repressor of several chromosomal genes involved in the SOS response in Escherichia coli. In previous experiments, we found that LexA protein may also be a repressor of the colicin E1 gene. We now present evidence that the purified LexA protein strongly repressed the in vitro transcription of the colicin E1 gene. As determined in DNase
Yousuke Ebina +4 more
openaire +3 more sources
Molecular Oncology, EarlyView.This study investigates an alternative approach to reactivating the oncosuppressor p53 in cancer. A short peptide targeting the association of the two p53 inhibitors, MDM2 and MDM4, induces an otherwise therapeutically active p53 with unique features that promote cell death and potentially reduce toxicity towards proliferating nontumor cells.
Sonia Valentini +10 more
wiley +1 more source
Coarse-grained model of entropic allostery [PDF]
, 2004 Many signaling functions in molecular biology require proteins to bind to substrates such as DNA in response to environmental signals such as the simultaneous binding to a small molecule. Examples are repressor proteins which may transmit information via
A. Cooper +13 more
core +3 more sources
Purification and properties of the biotin repressor. A bifunctional protein.
Journal of Biological Chemistry, 1982 Definitive evidence is presented for the bifunctional nature of the biotin repressor protein which possesses both regulatory and enzymatic activities. The repressor protein can activate biotin in the presence of ATP to form biotinyl-5'-adenylate, the co-repressor which remains tightly bound to the repressor protein.
Om Prakash, Max A. Eisenberg, S C Hsiung
openaire +3 more sources
Molecular Oncology, EarlyView.TGF‐β has a complex role in cancer, exhibiting both tumor‐suppressive and tumor‐promoting properties. Using a series of differentiated tumoroids, derived from different stages and mutational background of colorectal cancer patients, we replicate this duality of TGF‐β in vitro. Notably, the atypical but highly aggressive KRASQ22K mutation rendered early‐
Theresia Mair +17 more
wiley +1 more source