Results 41 to 50 of about 25,223,622 (264)

Phase variable restriction–modification systems inMoraxella catarrhalis [PDF]

FEMS Immunology & Medical Microbiology, 2002
A repetitive DNA motif was used as a marker to identify novel genes in the mucosal pathogen Moraxella catarrhalis. There is a high prevalence of such repetitive motifs in virulence genes that display phase variable expression. Two repeat containing loci were identified using a digoxigenin-labelled 5'-(CAAC)6-3' oligonucleotide probe.
Seib, K. L., Peak, I. R. A., Jennings, M. P.   +2 more
openaire   +5 more sources

Reassembling a cannon in the DNA defense arsenal: Genetics of StySA, a BREX phage exclusion system in Salmonella lab strains.

PLoS Genetics, 2022
Understanding mechanisms that shape horizontal exchange in prokaryotes is a key problem in biology. A major limit on DNA entry is imposed by restriction-modification (RM) processes that depend on the pattern of DNA modification at host-specified sites ...
Julie Zaworski, Oyut Dagva, Julius Brandt, Chloé Baum, Laurence Ettwiller, Alexey Fomenkov, Elisabeth A Raleigh   +6 more
doaj   +1 more source

Characterization of the NgoAXP: phase-variable type III restriction–modification system inNeisseria gonorrhoeae [PDF]

FEMS Microbiology Letters, 2009
Methyltransferases associated with type III restriction-modification (RM) systems are phase-variably expressed in a variety of pathogenic bacteria. NgoAXP, the type III RM system encoded by Neisseria gonorrhoeae, was characterized in this study. The cloned resngoAXP and ngoAXPmod genes were expressed in Escherichia coli strains.
Monika, Adamczyk-Poplawska, Michał, Lower, Andrzej, Piekarowicz   +2 more
openaire   +2 more sources

A Type I Restriction-Modification System Associated with Enterococcus faecium Subspecies Separation. [PDF]

Appl Environ Microbiol, 2019
Enterococcus faecium is a leading cause of hospital-acquired infections around the world. Rising antibiotic resistance in certain E. faecium lineages leaves fewer treatment options.
Huo W, Adams HM, Trejo C, Badia R, Palmer KL.   +4 more
europepmc   +2 more sources

Controller protein of restriction-modification system Kpn2I affects transcription of its gene by acting as a transcription elongation roadblock. [PDF]

Nucleic Acids Res, 2018
C-proteins control restriction–modification (R–M) systems’ genes transcription to ensure sufficient levels of restriction endonuclease to allow protection from foreign DNA while avoiding its modification by excess methyltransferase. Here, we characterize
Klimuk E, Bogdanova E, Nagornykh M, Rodic A, Djordjevic M, Medvedeva S, Pavlova O, Severinov K.   +7 more
europepmc   +2 more sources

Comparative genome and methylome analysis reveals restriction/modification system diversity in the gut commensal Bifidobacterium breve. [PDF]

Nucleic Acids Res, 2018
Bifidobacterium breve represents one of the most abundant bifidobacterial species in the gastro-intestinal tract of breast-fed infants, where their presence is believed to exert beneficial effects.
Bottacini F, Morrissey R, Roberts RJ, James K, van Breen J, Egan M, Lambert J, van Limpt K, Knol J, Motherway MO, van Sinderen D.   +10 more
europepmc   +2 more sources

Functional comparison of anti-restriction and anti-methylation activities of ArdA, KlcA, and KlcAHS from Klebsiella pneumoniae

Frontiers in Cellular and Infection Microbiology, 2022
Anti-restriction proteins are typically encoded by plasmids, conjugative transposons, or phages to improve their chances of entering a new bacterial host with a type I DNA restriction and modification (RM) system.
Huimin Chen, Shuan Tao, Na Li, Fang Wang, Lei Wang, Yu Tang, Wei Liang   +6 more
doaj   +1 more source

Impact of target site distribution for Type I restriction enzymes on the evolution of methicillin-resistant Staphylococcus aureus (MRSA) populations. [PDF]

, 2013
A limited number of Methicillin-resistant Staphylococcus aureus (MRSA) clones are responsible for MRSA infections worldwide, and those of different lineages carry unique Type I restriction-modification (RM) variants.
Dryden, David T. F., White, John H., Stephanou, AS, Cooper, Laurie P., Cooper, LP, Houston, PJ, Lindsay, JA, Chen, K, Dryden, DT, Roberts, Gareth A., Lindsay, Jodi A., Chen, Kai, Roberts, GA, White, JH, Stephanou, Augoustinos S., Houston, Patrick J.   +15 more
core   +1 more source

Generation of a restriction minus enteropathogenic Escherichia coli E2348/69 strain that is efficiently transformed with large, low copy plasmids

BMC Microbiology, 2008
Background Many microbes possess restriction-modification systems that protect them from parasitic DNA molecules. Unfortunately, the presence of a restriction-modification system in a given microbe also hampers genetic analysis.
Ward Jordan D, Price Nancy L, Hobson Neil, Raivio Tracy L   +3 more
doaj   +1 more source

hsdS, Belonging to the Type I Restriction-Modification System, Contributes to the Streptococcus suis Serotype 2 Survival Ability in Phagocytes. [PDF]

Front Microbiol, 2017
Streptococcus suis serotype 2 (SS2) is an important zoonotic agent in swine and humans. Anti-phagocytosis and survival in phagocytic cells and whole blood is essential for bacteria to be pathogenic.
Xu B, Zhang P, Li W, Liu R, Tang J, Fan H.   +5 more
europepmc   +2 more sources