Results 31 to 40 of about 18,327,370 (242)

Structural basis for broad anti-phage immunity by DISARM

Nature Communications, 2022
DISARM (Defense Island System Associated with Restriction Modification) systems can provide bacteria with protection against a wide range of phage. Here, Bravo et al.
Jack P. K. Bravo, Cristian Aparicio-Maldonado, Franklin L. Nobrega, Stan J. J. Brouns, David W. Taylor   +4 more
doaj   +1 more source

Kinetic Basis of the Bifunctionality of SsoII DNA Methyltransferase

Molecules, 2018
Type II restriction–modification (RM) systems are the most widespread bacterial antiviral defence mechanisms. DNA methyltransferase SsoII (M.SsoII) from a Type II RM system SsoII regulates transcription in its own RM system in addition to the methylation
Nadezhda A. Timofeyeva, Alexandra Yu. Ryazanova, Maxim V. Norkin, Tatiana S. Oretskaya, Olga S. Fedorova, Elena A. Kubareva   +5 more
doaj   +1 more source

Adaptive Tracking Control of an Electro-Pneumatic Clutch Actuator [PDF]

Modeling, Identification and Control, 2003
This paper deals with the application of a simple adaptive algorithm for robust tracking control of an electro-pneumatic clutch actuator with output feedback.
Glenn-Ole Kaasa, Masanori Takahashi
doaj   +1 more source

Rapid PCR Detection of Staphylococcus aureus Clonal Complex 398 by Targeting the Restriction-Modification System Carrying sau1-hsdS1 [PDF]

, 2011
A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this
Broens, Els M., Moodley, Arshnee, Guardabassi, L., Marc Stegger, Jodi A. Lindsay, Arshnee Moodley, Lindsay, J.A., Stegger, Marc, Broens, E.M., Els M. Broens, Skov, Robert, Moodley, A., Lindsay, Jodi A., Guardabassi, Luca, Skov, R., Robert Skov, Stegger, M., Luca Guardabassi   +17 more
core   +1 more source

Avoidance of recognition sites of restriction-modification systems is a widespread but not universal anti-restriction strategy of prokaryotic viruses

BMC Genomics, 2018
Background Restriction-modification (R-M) systems protect bacteria and archaea from attacks by bacteriophages and archaeal viruses. An R-M system specifically recognizes short sites in foreign DNA and cleaves it, while such sites in the host DNA are ...
I. S. Rusinov, A. S. Ershova, A. S. Karyagina, S. A. Spirin, A. V. Alexeevski   +4 more
doaj   +1 more source

Epigenetic Regulation of Virulence and Immunoevasion by Phase-Variable Restriction-Modification Systems in Bacterial Pathogens

, 2020
Human-adapted bacterial pathogens use a mechanism called phase variation to randomly switch the expression of individual genes to generate a phenotypically diverse population to adapt to challenges within and between human hosts.
Atack, JM, Srikhanta, YN, Jennings, MP, Seib, KL   +3 more
core   +1 more source

Burkholderia pseudomallei sequencing identifies genomic clades with distinct recombination, accessory, and epigenetic profiles. [PDF]

, 2014
Burkholderia pseudomallei (Bp) is the causative agent of the infectious disease melioidosis. To investigate population diversity, recombination, and horizontal gene transfer in closely related Bp isolates, we performed whole-genome sequencing (WGS) on ...
Studholme, David J., Holden, Mathew TG, Ong, C, Beacham, I, Parkhill, J, Harting, J, Chen, Swaine L., Mehershahi, K, Holden, Matthew T. G., Nandi, T, T.G. Holden, Matthew, Didelot, X, Peak, I, Baybayan, Primo, Peak, Ian, Sim, B, Hoon, CH, Nandi, Tannistha, Boddey, J, Boddey, Justin, Boddey, Justin A., Holden, Matthew, J. Studholme, David, Baybayan, P, Michell, S, Essex-Lopresti, AE, Guo, Yan, Sim, Bernice, Didelot, Xavier, Titball, Richard W., Chen, SL, Smither, Sophie, Smiters, Sophie, Beacham, Ifor, Holden, Matthew TG, Smither, S, How, LC, Nelson, Michelle, Ong, Catherine, Tan, P, Wang, S, Aw, LT, Nelson, M, Mitchell, Stephen, Michell, Stephen, Aw, Lay Tin, E. Essex-Lopresti, Angela, Chen, Swaine, Chen, Swaine L, Titball, Richard, Mehrashahi, Kurosh, Hoon, Chua Hui, Boddey, Justin A, Sarkar-Tyson, M, Parkhill, Julian, Mehershahi, Kurosh, Guo, Y, Tan, Patrick, How, Lee Chee, W. Titball, Richard, Sarkar-Tyson, Mitali, Wang, Susana, Essex-Lopresti, Angela, Studholme, David J, Harting, John   +64 more
core   +1 more source

SDM-assist software to design site-directed mutagenesis primers introducing "silent" restriction sites [PDF]

, 2013
BACKGROUND:Over the past decades site-directed mutagenesis (SDM) has become an indispensable tool for biological structure-function studies. In principle, SDM uses modified primer pairs in a PCR reaction to introduce a mutation in a cDNA insert.
Karnik, Abhijit, Grefen, Christopher, Karnik, Rucha   +2 more
core   +4 more sources

Engineering clostridium strain to accept unmethylated DNA. [PDF]

PLoS ONE, 2010
It is difficult to genetically manipulate the medically and biotechnologically important genus Clostridium due to the existence of the restriction and modification (RM) systems. We identified and engineered the RM system of a model clostridial species, C.
Hongjun Dong, Yanping Zhang, Zongjie Dai, Yin Li   +3 more
doaj   +1 more source

European Standard Clinical Practice Guideline and EXPeRT Recommendations for the Diagnosis and Management of Gastroenteropancreatic Neuroendocrine Neoplasms in Children and Adolescents

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Pediatric gastroenteropancreatic neuroendocrine neoplasms (GEP‐NENs) are extremely rare and clinically heterogeneous. Management has largely been extrapolated from adult practice. This European Standard Clinical Practice Guideline (ESCP), developed by the EXPeRT network in collaboration with adult NEN experts, provides (adult) evidence ...
Michaela Kuhlen, Calogero Virgone, Antje Redlich, Michael Abele, Ricardo Lopez‐Almaraz, Tal Ben‐Ami, Ewa Bien, Gianni Bisogno, Maja Mazic Cesen, Andrea Ferrari, Malgorzata A. Krawczyk, Yves Reguerre, Dominik T. Schneider, Ines B. Brecht, Charlotte Rigaud, Bilgehan Yalcin, Rainer Claus, Christian Vokuhl, Constantin Lapa, Jörg Fuchs, Segolene Hescot, Thomas Walter, Julien Hadoux, Daniel Orbach   +23 more
wiley   +1 more source