Deletion of the Zinc Transporter Lipoprotein AdcAII Causes Hyperencapsulation of
mBio, 2020 The capsule is the dominant Streptococcus pneumoniae virulence factor, yet how variation in capsule thickness is regulated is poorly understood. Here, we describe an unexpected relationship between mutation of adcAII, which encodes a zinc uptake ...
Claire Durmort +11 more
doaj +3 more sources
A mobile restriction modification system consisting of methylases on the IncA/C plasmid [PDF]
Mobile DNA, 2019 Background IncA/C plasmids play important roles in the development and dissemination of multidrug resistance in bacteria. These plasmids carry three methylase genes, two of which show cytosine specificity.
Ruibai Wang, Jing Lou, Jie Li
doaj +3 more sources
Temporal dynamics of methyltransferase and restriction endonuclease accumulation in individual cells after introducing a restriction-modification system. [PDF]
Nucleic Acids Res, 2016 © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. Type II restriction-modification (R-M) systems encode a restriction endonuclease that cleaves DNA at specific sites, and a methyltransferase that modifies ...
Morozova N +9 more
europepmc +3 more sources
Restriction-modification system with methyl-inhibited base excision and abasic-site cleavage activities. [PDF]
Nucleic Acids Res, 2015 The restriction-modification systems use epigenetic modification to distinguish between self and nonself DNA. A modification enzyme transfers a methyl group to a base in a specific DNA sequence while its cognate restriction enzyme introduces breaks in ...
Fukuyo M +9 more
europepmc +3 more sources
Plasmid pEC156, a Naturally Occurring Escherichia coli Genetic Element That Carries Genes of the EcoVIII Restriction-Modification System, Is Mobilizable among Enterobacteria. [PDF]
PLoS ONE, 2016 Type II restriction-modification systems are ubiquitous in prokaryotes. Some of them are present in naturally occurring plasmids, which may facilitate the spread of these systems in bacterial populations by horizontal gene transfer.
Olesia Werbowy, Tadeusz Kaczorowski
doaj +2 more sources
Delineation of a lactococcal conjugation system reveals a restriction‐modification evasion system
Microbial Biotechnology, 2023 Plasmid pUC11B is a 49.3‐kb plasmid harboured by the fermented meat isolate Lactococcus lactis subsp. lactis UC11. Among other features, pUC11B encodes a pMRC01‐like conjugation system and tetracycline‐resistance.
Guillermo Ortiz Charneco +8 more
doaj +2 more sources
From sequence to activity: the HgaI-homologous restriction modification system RM.MhoVI of Mycoplasma hominis [PDF]
BMC MicrobiologyRestriction-modification (RM) systems are widespread defense mechanisms in prokaryotes that protect the host from potentially harmful foreign DNA. They typically consist of a DNA methyltransferase (MTase), which methylates the host genome at an adenine ...
Lars Vogelgsang +5 more
doaj +2 more sources
A Type III restriction–modification system in Mycoplasma mycoides subsp. capri [PDF]
Open Biology, 2012 The sequenced genome of Mycoplasma mycoides subsp. capri revealed the presence of a Type III restriction–modification system (MmyCI). The methyltransferase (modification) subunit of MmyCI (M.MmyCI) was shown to recognize the sequence 5′-TGAG-3′ and ...
Mikkel A. Algire +4 more
doaj +2 more sources
An alternative approach to study the enzymatic specificities of the CfrBI restriction–modification system [PDF]
Heliyon, 2019 Restriction–modification systems (RMS) are the main gene-engineering tools and a suitable model to study the molecular mechanisms of catalysis and DNA–protein interactions.
Marina V. Zakharova +3 more
doaj +2 more sources
MspGI Restriction–Modification System and Its Flanking Genes of Microbacterium sp. Gd 4-13
Biology and Life Sciences ForumBacteria have defense systems against the penetration of foreign DNA (phages and plasmids). Some defense systems protect bacteria from phage penetration into the cell, while others block phage replication. Antiphage defense systems are often located next
Alfiya K. Yunusova +3 more
doaj +2 more sources