Results 21 to 30 of about 25,223,622 (264)

The restriction impacts of the Type III restriction-modification system on the transmission dynamics of antimicrobial resistance genes in Campylobacter jejuni [PDF]

Frontiers in Microbiology
IntroductionThe spread of antibiotic resistance genes among Campylobacter jejuni (C. jejuni) is a serious problem, and the effects of the restriction-modification (R-M) system on the transmission dynamics of these genes in C.
Yu Qiu, Pengbo Guo, Hui Tian, Ye Zhou, Hongling Wen, Hao Liang   +5 more
doaj   +2 more sources

Bacterial autoimmunity due to a restriction-modification system

Current Biology, 2016
Restriction-modification (RM) systems represent a minimal and ubiquitous biological system of self/non-self discrimination in prokaryotes [1], which protects hosts from exogenous DNA [2]. The mechanism is based on the balance between methyltransferase (M)
Bergmiller, Tobias ; https://orcid.org/, Wakamoto, Yuichi, Guet, Calin C ; https://orcid.org/, Kussell, Edo, Okura, Reiko, Pleska, Maros ; https://orcid.org/, Qian, Long   +6 more
core   +2 more sources

Development of a CRISPR-based cytosine base editor for restriction-modification system inactivation to enhance transformation efficiency in Vibrio Sp. dhg [PDF]

Journal of Biological Engineering
Background Vibrio sp. dhg is a fast-growing, alginate-utilizing, marine bacterium being developed as a platform host for macroalgae biorefinery. To maximize its potential in the production of various value-added products, there is a need to expand ...
Yang Jun Shon, Dongyeop Baek, Su Bin Jin, Woo Jae Kim, Gyoo Yeol Jung, Hyun Gyu Lim   +5 more
doaj   +2 more sources

A mobile restriction-modification system provides phage defence and resolves an epigenetic conflict with an antagonistic endonuclease. [PDF]

Nucleic Acids Res, 2022
Epigenetic DNA methylation plays an important role in bacteria by influencing gene expression and allowing discrimination between self-DNA and intruders such as phages and plasmids. Restriction–modification (RM) systems use a methyltransferase (MTase) to
Birkholz N, Jackson SA, Fagerlund RD, Fineran PC.   +3 more
europepmc   +2 more sources

Broadness and specificity: ArdB, ArdA, and Ocr against various restriction-modification systems

Frontiers in Microbiology, 2023
ArdB, ArdA, and Ocr proteins inhibit the endonuclease activity of the type I restriction-modification enzymes (RMI). In this study, we evaluated the ability of ArdB, ArdA, and Ocr to inhibit different subtypes of Escherichia coli RMI systems (IA, IB, and
Anna A. Kudryavtseva, Eva Cséfalvay, Evgeniy Yu Gnuchikh, Darya D. Yanovskaya, Mikhail A. Skutel, Artem B. Isaev, Sergey V. Bazhenov, Sergey V. Bazhenov, Sergey V. Bazhenov, Anna A. Utkina, Ilya V. Manukhov, Ilya V. Manukhov, Ilya V. Manukhov   +12 more
doaj   +2 more sources

Effects of Population Dynamics on Establishment of a Restriction-Modification System in a Bacterial Host [PDF]

Molecules, 2019
In vivo dynamics of protein levels in bacterial cells depend on both intracellular regulation and relevant population dynamics. Such population dynamics effects, e.g., interplay between cell and plasmid division rates, are, however, often neglected in ...
Stefan Graovac, Andjela Rodic, Magdalena Djordjevic, Konstantin Severinov, Marko Djordjevic   +4 more
doaj   +2 more sources

Dependence of post-segregational killing mediated by Type II restriction–modification systems on the lifetime of restriction endonuclease effective activity

mBio
Plasmid-borne Type II restriction–modification (RM) systems mediate post-segregational killing (PSK). PSK is thought to be caused by the dilution of restriction and modification enzymes during cell division, resulting in accumulation of unmethylated DNA ...
Svetlana Kozlova, Natalia Morozova, Yaroslav Ispolatov, Konstantin Severinov   +3 more
doaj   +2 more sources

Improved transformation efficiency of group A Streptococcus by inactivation of a type I restriction modification system. [PDF]

PLoS One, 2021
Streptococcus pyogenes or group A Streptococcus (GAS) is a leading cause of bacterial pharyngitis, skin and soft tissue infections, life-threatening invasive infections, and the post-infectious autoimmune syndromes of acute rheumatic fever and post ...
Finn MB, Ramsey KM, Tolliver HJ, Dove SL, Wessels MR.   +4 more
europepmc   +2 more sources

A Type I Restriction Modification System Influences Genomic Evolution Driven by Horizontal Gene Transfer in Paenibacillus polymyxa. [PDF]

Front Microbiol, 2021
Considered a “Generally Recognized As Safe” (GRAS) bacterium, the plant growth–promoting rhizobacterium Paenibacillus polymyxa has been widely applied in agriculture and animal husbandry.
Chen Z, Shen M, Mao C, Wang C, Yuan P, Wang T, Sun D.   +6 more
europepmc   +2 more sources

Systematic Analysis of REBASE Identifies Numerous Type I Restriction-Modification Systems with Duplicated, Distinct hsdS Specificity Genes That Can Switch System Specificity by Recombination

mSystems, 2020
N6-Adenine DNA methyltransferases associated with some Type I and Type III restriction-modification (R-M) systems are able to undergo phase variation, randomly switching expression ON or OFF by varying the length of locus-encoded simple sequence repeats (
Yang, Long, Zhou, Yaoqi, Litfin, Thomas, Guo, Chengying, Atack, John M, Jennings, Michael P, Blackall, Patrick J   +6 more
core   +2 more sources