One-pot Golden Gate Assembly of an avian infectious bronchitis virus reverse genetics system. [PDF]
PLoS ONEAvian infectious bronchitis is an acute respiratory disease of poultry of particular concern for global food security. Investigation of infectious bronchitis virus (IBV), the causative agent of avian infectious bronchitis, via reverse genetics enables ...
Katharina Bilotti +13 more
doaj +2 more sources
Efficient and Robust
mSphere, 2017 The notoriously low efficiency of Paramyxoviridae reverse genetics systems has posed a limiting barrier to the study of viruses in this family. Previous approaches to reverse genetics have utilized a wide variety of techniques to overcome the technical ...
Shannon M. Beaty +9 more
doaj +2 more sources
Generation of an artificially attenuated fowl adenovirus 4 viral vector using the reverse genetics system based on full-length infectious clone. [PDF]
Vet ResTang Z, Feng D, Fan W, Yan L, Song S.
europepmc +3 more sources
Embryo-scale reverse genetics at single-cell resolution. [PDF]
Nature, 2023 We present the ‘zebrafish single-cell atlas of perturbed embryos’, single-cell trancriptomic data of developing zebrafish embryos across various timepoints and with genetic perturbations.
Saunders LM +10 more
europepmc +2 more sources
Reverse-genetics studies of lncRNAs—what we have learnt and paths forward [PDF]
Genome Biology, 2020 Long non-coding RNAs (lncRNAs) represent a major fraction of the transcriptome in multicellular organisms. Although a handful of well-studied lncRNAs are broadly recognized as biologically meaningful, the fraction of such transcripts out of the entire ...
Fan Gao +3 more
openalex +2 more sources
PLoS Biology, 2021 The recent emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the underlying cause of Coronavirus Disease 2019 (COVID-19), has led to a worldwide pandemic causing substantial morbidity, mortality, and economic devastation.
Suzannah J. Rihn +82 more
semanticscholar +1 more source
Development of a rapid reverse genetics system for feline coronavirus based on TAR cloning in yeast
Frontiers in Microbiology, 2023 Introduction Reverse genetics has become an indispensable tool to gain insight into the pathogenesis of viruses and the development of vaccines.
Hongmin Cao +3 more
semanticscholar +1 more source
Reverse genetics systems for SARS‐CoV‐2
Journal of Medical Virology, 2022 The ongoing pandemic of coronavirus disease 2019 (COVID‐19) has caused severe public health crises and heavy economic losses. Limited knowledge about this deadly virus impairs our capacity to set up a toolkit against it.
Wenhao Wang +4 more
semanticscholar +1 more source
Genomic introgression mapping of field-derived multiple-anthelmintic resistance in Teladorsagia circumcincta [PDF]
, 2017 Preventive chemotherapy has long been practiced against nematode parasites of livestock, leading to widespread drug resistance, and is increasingly being adopted for eradication of human parasitic nematodes even though it is similarly likely to lead to ...
Bisset, Stewart A +6 more
core +17 more sources
A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses
Nature Communications, 2021 The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We demonstrate that despite the large size of the viral RNA genome (~30 kb), infectious full-length cDNA is readily assembled in vitro by a ...
Alberto A. Amarilla +30 more
semanticscholar +1 more source