Results 51 to 60 of about 663,476 (278)

Occurrence of LINE, gypsy-like, and copia-like retrotransposons in the clonally propagated sweet potato (Ipomoea batatas L.) [PDF]

open access: yes, 2011
Retrotransposons are a class of transposable elements that represent a major fraction of the repetitive DNA of most eukaryotes. Their abundance stems from their expansive replication strategies.
Dieters, Mark   +3 more
core   +2 more sources

Reciprocal control of viral infection and phosphoinositide dynamics

open access: yesFEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Development of a PCR-Based Reverse Genetics System for an Attenuated Duck Tembusu Virus Strain. [PDF]

open access: yesPLoS ONE, 2016
The infectious disease caused by the duck Tembusu virus (DTMUV) has resulted in massive economic losses to the Chinese duck industry in China since 2010.
Xiaogang Wu   +12 more
doaj   +1 more source

Modern Approaches to Molecular Genetics of Viruses in the Study of the Members of the Family Coronaviridae

open access: yesВестник войск РХБ защиты, 2021
The existence of certain suspicions about the artificial origin of the COVID-19 pandemic and about the possible use of reverse genetics technology to create the SARS-CoV-2 virus require an understanding of its capabilities in the design of new viruses ...
M. V. Supotnitskiy
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

open access: yesFEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho   +3 more
wiley   +1 more source

The reverse genetics applied to fish RNA viruses

open access: yesVeterinary Research, 2011
Aquaculture has expanded rapidly to become a major economic and food-producing sector worldwide these last 30 years. In parallel, viral diseases have emerged and rapidly spread from farm to farm causing enormous economic losses.
Biacchesi Stéphane
doaj   +1 more source

Reverse genetics of rotavirus [PDF]

open access: yesProceedings of the National Academy of Sciences, 2017
The genetics of viruses are determined by mutations of their nucleic acid. Mutations can occur spontaneously or be produced by physical or chemical means: for example, the application of different temperatures or mutagens (such as hydroxylamine, nitrous acid, or alkylating agents) that alter the nucleic acid.
openaire   +2 more sources

Evaluation of Stem-Loop Reverse Transcription and Poly-A Tail Extension in MicroRNA Analysis of Body Fluids [PDF]

open access: yes, 2014
MicroRNA has been demonstrated to be a viable tool for body fluid identification purposes in forensic casework. Stem-loop reverse transcription (slRT) is regularly used for cDNA synthesis from mature miRNA, along with poly-A tail extension.
Dunnett, Hannah   +2 more
core   +1 more source

By dawn or dusk—how circadian timing rewrites bacterial infection outcomes

open access: yesFEBS Letters, EarlyView.
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo   +2 more
wiley   +1 more source

Reverse genetics systems of plant negative-strand RNA viruses are difficult to be developed but powerful for virus-host interaction studies and virus-based vector applications

open access: yesPhytopathology Research, 2020
Plant virus-induced diseases cause significant losses to agricultural crop production worldwide. Reverse genetics systems of plant viruses allow gene manipulation on viral genomes, which greatly facilitates studies of viral pathogenesis and interactions ...
Ying Zang   +4 more
doaj   +1 more source

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