Results 51 to 60 of about 666,471 (318)

Mapping of transcription termination within the S segment of SFTS phlebovirus facilitated the generation of NSs-deletant viruses [PDF]

, 2017
SFTS phlebovirus (severe fever with thrombocytopenia syndrome virus; SFTSV) is an emerging tick-borne bunyavirus that was first reported in China in 2009. Here we report the generation of a recombinant SFTSV (rHB29NSsKO) that cannot express the viral non-
Brennan, Benjamin, Elliott, Richard M., Rezelj, Veronica V.   +2 more
core   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Development of a PCR-Based Reverse Genetics System for an Attenuated Duck Tembusu Virus Strain. [PDF]

PLoS ONE, 2016
The infectious disease caused by the duck Tembusu virus (DTMUV) has resulted in massive economic losses to the Chinese duck industry in China since 2010.
Xiaogang Wu, Ying Shi, Dawei Yan, Xuesong Li, Pixi Yan, Xuyuan Gao, Yuee Zhang, Lei Yu, Chaochao Ren, Guoxin Li, Liping Yan, Qiaoyang Teng, Zejun Li   +12 more
doaj   +1 more source

Modern Approaches to Molecular Genetics of Viruses in the Study of the Members of the Family Coronaviridae

Вестник войск РХБ защиты, 2021
The existence of certain suspicions about the artificial origin of the COVID-19 pandemic and about the possible use of reverse genetics technology to create the SARS-CoV-2 virus require an understanding of its capabilities in the design of new viruses ...
M. V. Supotnitskiy
doaj   +1 more source

Plasmodium falciparum gametogenesis essential protein 1 (GEP1) is a transmission‐blocking target

FEBS Letters, EarlyView.
This study shows Plasmodium falciparum GEP1 is vital for activating sexual stages of malarial parasites even independently of a mosquito factor. Knockout parasites completely fail gamete formation even when a phosphodiesterase inhibitor is added. Two single‐nucleotide polymorphisms (V241L and S263P) are found in 12%–20% of field samples.
Frederik Huppertz, Milagros Siebeck Caturelli, Lina S. Lehmann, Florian Kurth, Alexander G. Maier, Kai Matuschewski   +5 more
wiley   +1 more source

Mutations in the Schmallenberg virus Gc glycoprotein facilitate cellular protein synthesis shutoff and restore pathogenicity of NSs deletion mutants in mice [PDF]

, 2016
Serial passage of viruses in cell culture has been traditionally used to attenuate virulence and identify determinants of viral pathogenesis. In a previous study, we found that a strain of Schmallenberg virus (SBV) serially passaged in tissue culture ...
Aislynn Taggart, Anna Janowicz, BMJ Group, Frauke Seehusen, Ilaria M. Piras, Kerstin Hahn, Marco Caporale, Mariana Varela, Massimo Palmarini, Rute Maria Pinto, S. López, William Marciel de Souza, Wolfgang Baumgärtner, Xiaohong Shi   +13 more
core   +1 more source

Linked dimers of the AAA+ ATPase Msp1 reveal energetic demands and mechanistic plasticity for substrate extraction from lipid bilayers

FEBS Letters, EarlyView.
Cells must clear mislocalized or faulty proteins from membranes to survive. The AAA+ ATPase Msp1 performs this task, but dissecting how its six subunits work together is challenging. We engineered linked dimers with varied numbers of functional subunits to reveal how Msp1 subunits cooperate and use energy to extract proteins from the lipid bilayer ...
Deepika Gaur, Brian Acquaviva, Baylee A. Smith, Nathan Walker, Isabella Walter, Matthew L. Wohlever   +5 more
wiley   +1 more source

Variation in the flowering time orthologs BrFLC and BrSOC1 in a natural population of Brassica rapa. [PDF]

, 2015
Understanding the genetic basis of natural phenotypic variation is of great importance, particularly since selection can act on this variation to cause evolution.
Franks, Steven J, Jordan, Kevin P, Lalchan, Rebecca, Litt, Amy, Nowogrodzki, Anna, Perez-Sweeney, Beatriz, Strahl, Maya, Weber, Jennifer J   +7 more
core   +2 more sources

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Reverse genetics systems of plant negative-strand RNA viruses are difficult to be developed but powerful for virus-host interaction studies and virus-based vector applications

Phytopathology Research, 2020
Plant virus-induced diseases cause significant losses to agricultural crop production worldwide. Reverse genetics systems of plant viruses allow gene manipulation on viral genomes, which greatly facilitates studies of viral pathogenesis and interactions ...
Ying Zang, Xiao-Dong Fang, Ji-Hui Qiao, Qiang Gao, Xian-Bing Wang   +4 more
doaj   +1 more source