Results 81 to 90 of about 3,000 (187)

Rifampicin curing of plasmids in Escherichia coli K12−rifampicin resistant host

Journal of Pharmacy and Pharmacology, 1984
Abstract The in-vitro rifampicin curing of R-plasmids JR225, BN102, BN106 and F’-plasmid F’lac+/R386 in Escherichia coli K12 rifampicin-resistant host J62–2 is described. The minimum rifampicin curing concentration and the frequency of curing of plasmids from the rifampicin-resistant host cell J62–2 and the isogenic rifampicin-sensitive ...
openaire   +2 more sources

The combination of morphogenic regulators BABY BOOM and GRF‐GIF improves maize transformation efficiency and promotes leaf regeneration

New Phytologist, EarlyView.
Maize regeneration via somatic embryogenesis by ectopic expression of morphogenic factors TaGRF4‐TaGIF1 and ZmBBM. Summary Transformation is an indispensable tool for plant genetics and functional genomics. Although stable transformation in maize is no longer a major obstacle, there remains a need for accessible and efficient methods for academic ...
Zongliang Chen, Jiaqi Zhou, Mary Galli, Sessen Daniel Iohannes, Theresa Clark, Juan M. Debernardi, Jorge Dubcovsky, David Jackson, Andrea Gallavotti   +8 more
wiley   +1 more source

Prevalence of rifampicin resistance in pulmonary tuberculosis: A laboratory-based study.

PLOS Global Public Health
Tuberculosis (TB) remains a significant global public health challenge, particularly in low- and middle-income countries. The emergence of rifampicin-resistant tuberculosis further complicates control efforts.
Israel Bedzina, Agnes Naa Larley Lartey, Lois Kwaley-Buabeng, Prosper Mensah, Gameli Nudo, Eric Selorm Odum, Rita Sewornu, Kenneth Ablordey, John Gameli Deku, Kwabena Obeng Duedu   +9 more
doaj   +1 more source

Knocking Out Two Polyphenol Oxidase Genes Significantly Improves Recombinant Protein Purification in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Efficient purification remains a key technical challenge affecting the recovery efficiency of recombinant proteins in plant‐based systems. Complex metabolites, particularly polyphenols, often cause recombinant protein aggregation during purification.
Hai‐Ping Diao, Hui‐Xin Meng, Xue‐Jiao Xu, Zeng‐Lin Zhang, Jian‐Feng Zhang, Yong‐Feng Guo, Inhwan Hwang, Shi‐Jian Song   +7 more
wiley   +1 more source

Staurosporine Targets Mitochondrial Regulator VdAtuA3 to Disrupt Mitochondrial Homeostasis to Control Verticillium Wilt

Plant Biotechnology Journal, EarlyView.
ABSTRACT Mitochondria serve as cellular powerhouses that generate ATP via electron transport chain complexes and orchestrate metabolism‐apoptosis cross‐talk, yet genes maintaining mitochondrial homeostasis remain underexplored as antifungal targets. In this study, a previously uncharacterised protein VdAtuA3 was identified as a novel interactor with ...
Ruo‐Cheng Sheng, Huan Li, Jun Wang, Li‐Chao Wang, Hui‐Zi Liu, Xiao‐Feng Dai, Steven J. Klosterman, Jie‐Yin Chen, Krishna V. Subbarao, Feng‐Mao Chen, Dan‐Dan Zhang   +10 more
wiley   +1 more source

Multi‐Omics Analyses Reveal the Red and Far‐Red Light Combination Enhancing Heterologous Protein and Metabolite Production in Nicotiana benthamiana

Plant, Cell &Environment, EarlyView.
ABSTRACT Transient expression of exogenous protein in Nicotiana benthamiana leaves via agroinfiltration offers a rapid and efficient platform for functional gene discovery and heterologous production of valuable eukaryotic proteins and metabolites. Though light quality is an important factor for plant photomorphogenesis, its impact on the efficiency of
Yating Zhang, Fengjiao Wang, Ran Du, Tao Li, Shaoqun Zhou, Jianbin Yan, Wei Li   +6 more
wiley   +1 more source

Leaf Structure and Hormonal Balance Modulate Host‐Specificity and Pathogenicity of Botrytis cinerea

Plant, Cell &Environment, EarlyView.
ABSTRACT Botrytis cinerea, a highly successful necrotrophic fungal plant pathogen, demonstrates a remarkably broad host range, yet the precise factors governing its host specificity and its ability to coexist non‐pathogenically with plants remain unclear. This study systematically assessed host preference in a series of B. cinerea agricultural isolates,
Gautam Anand, Chen Benoni, Meirav Leibman‐Markus, Rupali Gupta, Iftah Marash, Loida Tejada, Tali Dolgov, Dalia Rav‐David, Ondřej Plíhal, Ondřej Novák, Maya Kleiman, Yigal Elad, Maya Bar   +12 more
wiley   +1 more source

Susceptibility Profiles of Mycobacterium ulcerans Isolates to Streptomycin and Rifampicin in Two Districts of the Eastern Region of Ghana

International Journal of Microbiology, 2016
Background. Drug resistance is a major challenge in antibiotic chemotherapy. Assessing resistance profiles of pathogens constitutes an essential surveillance tool in the epidemiology and control of infectious diseases, including Buruli ulcer (BU) disease.
Enid Owusu, Mercy Jemima Newman, Nana Konama Kotey, Amos Akumwena, Elizabeth Bannerman   +4 more
doaj   +1 more source

Evolution of resistance mechanisms and biological characteristics of rifampicin-resistant Staphylococcus aureus strains selected in vitro

BMC Microbiology, 2019
Background We aimed to determine the evolutionary pathways of rifampicin resistance in Staphylococcus aureus, and the impact of resistance mutations in the rpoB gene on fitness.
Chong Wang, Renchi Fang, Beibei Zhou, Xuebin Tian, Xiucai Zhang, Xiangkuo Zheng, Siqin Zhang, Guofeng Dong, Jianming Cao, Tieli Zhou   +9 more
doaj   +1 more source

Airborne Immunity: A Bacterial Volatile Primes Plant Defenses by Unlocking a Phytoalexin Biosynthetic Checkpoint

Plant, Cell &Environment, EarlyView.
ABSTRACT Microbial volatile organic compounds (mVOCs) enable plants to perceive microbial activity prior to physical contact, yet the contribution of individual bacterial volatiles to immune signalling and disease resistance remains incompletely understood.
Ivan A. Paponov, Chidananda Nagamangala Kanchiswamy, Simone Bossi, Francesco Caldo, Ambra S. Parmagnani, Mickael Malnoy, Massimo E. Maffei   +6 more
wiley   +1 more source