Results 1 to 10 of about 498,663 (149)
Architecture of the U5 small nuclear RNA. [PDF]
Molecular and Cellular Biology, 1994 We have used comparative sequence analysis and deletion analysis to examine the secondary structure of the U5 small nuclear RNA (snRNA), an essential component of the pre-mRNA splicing apparatus. The secondary structure of Saccharomyces cerevisiae U5 snRNA was studied in detail, while sequences from six other fungal species were included in the ...
Daniel N. Frank +2 more
openaire +3 more sources
Precursors of U4 small nuclear RNA. [PDF]
The Journal of cell biology, 1984 The processing and ribonucleoprotein assembly of U4 small nuclear RNA has been investigated in HeLa cells. After a 45-min pulse label with [3H]uridine, a set of apparently cytoplasmic RNAs was observed migrating just behind the gel electrophoretic position of mature U4 RNA.
Thoru Pederson +3 more
openaire +2 more sources
Transcription boundaries of U1 small nuclear RNA. [PDF]
Molecular and Cellular Biology, 1985 Transcription-proximal stages of U1 small nuclear RNA biosynthesis were studied by 32P labeling of nascent chains in isolated HeLa cell nuclei. Labeled RNA was hybridized to nitrocellulose-immobilized, single-stranded M13 DNA clones corresponding to regions within or flanking a human U1 RNA gene. Transcription of U1 RNA was inhibited by greater than 95%
Gary R. Kunkel, Thoru Pederson
openaire +2 more sources
Pseudouridine formation in U2 small nuclear RNA. [PDF]
Proceedings of the National Academy of Sciences, 1994 U2 small nuclear RNA contains 13 pseudouridine (psi) nucleotides, of which 11 are clustered in 5' regions involved in base-pairing interactions with other RNAs in the spliceosome. As a first step toward understanding the psi formation pathway in U2 RNA, we investigated psi formation on unmodified human U2 RNA in a HeLa cell extract system.
Thoru Pederson +2 more
openaire +2 more sources
U1 and U2 small nuclear RNAs are present in nuclear speckles. [PDF]
Proceedings of the National Academy of Sciences, 1992 The localization of U1 and U2 small nuclear RNAs (snRNAs) has been examined by in situ hybridization using 2'-O-alkyl oligonucleotide probes. We have found that these snRNAs, which are essential for pre-mRNA splicing, localize in a speckled distribution, in addition to being present in three of four foci, in HeLa cell nuclei.
Sui Huang, David L. Spector
openaire +3 more sources
Small nuclear RNAs in the ciliateTetrahymena
Nucleic Acids Research, 1985 We have isolated and partially characterized a family of small nuclear RNAs (snRNAs) from three different species of the protozoan Tetrahymena. We find six distinct snRNAs ranging in size from 100 to 250 nucleotides. The two largest snRNAs, as well as an abundant, heterogenous group of smaller snRNAs are found in the nucleolar RNA fraction. None of the
Per Hellung-Larsen +2 more
openaire +4 more sources
Evolutionary origin of the U6 small nuclear RNA intron. [PDF]
Molecular and Cellular Biology, 1990 U6 is the most conserved of the five small nuclear RNAs known to participate in pre-mRNA splicing. In the fission yeast Schizosaccharomyces pombe, the single-copy gene encoding this RNA is itself interrupted by an intron (T. Tani and Y. Ohshima, Nature (London) 337:87-90, 1989).
C Reich, Jo Ann Wise
openaire +3 more sources
U6 small nuclear RNA is transcribed by RNA polymerase III. [PDF]
Proceedings of the National Academy of Sciences, 1986 A DNA fragment homologous to U6 small nuclear RNA was isolated from a human genomic library and sequenced. The immediate 5'-flanking region of the U6 DNA clone had significant homology with a potential mouse U6 gene, including a "TATA box" at a position 26-29 nucleotides upstream from the transcription start site.
James P. Calvet +3 more
openaire +3 more sources
Nucleocytoplasmic Transport and Processing of Small Nuclear RNA Precursors [PDF]
Molecular and Cellular Biology, 1990 We have analyzed the structures and locations of small nuclear RNA (snRNA) precursors at various stages in their synthesis and maturation. In the nuclei of pulse-labeled Xenopus laevis oocytes, we detected snRNAs that were longer than their mature forms at their 3' ends by up to 10 nucleotides.
H E Neuman de Vegvar, James E. Dahlberg
openaire +3 more sources
Fluoropyrimidine-mediated changes in small nuclear RNA.
Journal of Biological Chemistry, 1986 Studies were completed to examine the effects of the antineoplastic agent 5-Fluorouridine (FUrd) on the metabolism of the small molecular weight nuclear RNA (snRNA). Cultured Sarcoma-180 murine tumor cells were exposed to FUrd concurrent with [3H]cytidine for 6 h, the drug was removed, and the RNA was isolated at 0, 24, or 48 h following the drug ...
R D Armstrong, C H Takimoto, E C Cadman
openaire +3 more sources